Proteome Profiler Mouse Cytokine Array Kit, Panel A
Proteome Profiler Mouse Cytokine Array Kit, Panel A Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected mouse cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and plasma.
Key Benefits
- Detects 40 mouse cytokines, chemokines, and acute phase proteins simultaneously
- Requires no specialized equipment
- Compatible with LI-COR* and chemiluminescence detection
Principle of the Assay
The Proteome Profiler Mouse Cytokine Array Kit, Panel A is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Multiple Cytokines?
Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.
- Rectangular 4-Well Multi-dish
- 4 Mouse Cytokine Array, Panel A nitrocellulose membranes spotted with 40 different antibodies to mouse cytokines, chemokines, and acute phase proteins
- Array Buffer 4
- Array Buffer 6
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Mouse Cytokine Array, Panel A
- Streptavidin-HRP
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample. | ||
CXCL13/BLC/BCA-1 | IL-5 | M-CSF |
C5a | IL-6 | CCL2/JE/MCP-1 |
G-CSF | IL-7 | CCL12/MCP-5 |
GM-CSF | IL-10 | CXCL9/MIG |
CCL1/I-309 | IL-12 p70 | CCL3/MIP-1 alpha |
CCL11/Eotaxin | IL-13 | CCL4/MIP-1 beta |
ICAM-1 | IL-16 | CXCL2/MIP-2 |
IFN-gamma | IL-17 | CCL5/RANTES |
IL-1 alpha/IL-1F1 | IL-23 | CXCL12/SDF-1 |
IL-1 beta/IL-1F2 | IL-27 | CCL17/TARC |
IL-1ra/IL-1F3 | CXCL10/IP-10 | TIMP-1 |
IL-2 | CXCL11/I-TAC | TNF-alpha |
IL-3 | CXCL1/KC | TREM-1 |
IL-4 |
Assays for Analytes represented in the Mouse Cytokine Array Kit, Panel A
Analyte | Quantikine® ELISA Kits | DuoSet® ELISA Development Systems |
CXCL13/BLC/BCA-1 | MCX130 | DY470 |
C5a | DY2150 | |
G-CSF | MCS00 | DY414 |
GM-CSF | MGM00 | DY415 |
CCL1/I-309/TCA-3 | DY845 | |
CCL11/Eotaxin | MME00 | DY420 |
ICAM-1 | MIC100 | DY796 |
IFN-gamma | MIF00 | DY485 |
IL-1 alpha | MLA00 | DY400 |
IL-1 beta | MLB00B | DY401 |
IL-1ra | MRA00 | DY480 |
IL-2 | M2000 | DY402 |
IL-3 | M3000 | DY403 |
IL-4 | M4000B | DY404 |
IL-5 | M5000 | DY405 |
IL-6 | M6000B | DY406 |
IL-7 | M7000 | DY407 |
IL-10 | M1000b | DY417 |
IL-12 p70 | M1270 | DY419 |
IL-13 | M1300CB | DY413 |
IL-16 | ||
IL-17 | M1700 | DY421 |
IL-23 | M2300 | DY1887 |
IL-27 | M2728 | |
CXCL10/IP-10/CRG-2 | MCX100 | DY466 |
CXCL11/I-TAC | DY572 | |
CXCL1/KC | MKC00B | DY453 |
M-CSF | MMC00 | DY416 |
CCL2/MCP-1/JE | MJE00 | DY479 |
CCL12/MCP-5 | MCC120 | DY428 |
CXCL9/MIG | MCX900 | DY492 |
CCL3/MIP-1 alpha | MMA00 | DY450 |
CCL4/MIP-1 beta | MMB00 | DY451 |
CXCL2/MIP-2 | MM200 | DY452 |
CCL5/RANTES | MMR00 | DY478 |
CXCL12/SDF-1 | MCX120 | DY460 |
CCL17/TARC | DY529 | |
TIMP-1 | MTM100 | DY980 |
TNF-alpha | MTA00 | DY410 |
TREM-1 | MTRM10 | DY1187 |
Specifications
Product Datasheets
Scientific Data
The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample. Mouse splenocytes treated with 100 ng/mL LPS for 20 hours were mixed with a cocktail of biotinylated detection antibodies, and then incubated with the Mouse Cytokine Array. The array was then incubated with streptavidin-horseradish peroxidase followed by chemiluminescent detection. After detection, the array data were quantitated to generate a protein profile (histogram). The table shows the analytes detected and their location on the membrane.
The Mouse Cytokine Array detects multiple analytes in cell culture lysates and supernates. (A. and B.) Lysates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µg of lysate was used for each array shown. (C. and D.) Supernates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µL of supernate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.
The Mouse Cytokine Array detects multiple analytes in mouse serum and tissue lysates. (A and B.) Samples of mouse serum from two different vendors. 200 µL of serum was used for each array shown. (C. and D.) Lysates of mouse lung tissue and mouse stomach tissue. 200 µg of lysate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.
Citations for Proteome Profiler Mouse Cytokine Array Kit, Panel A
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Heterotypic Cell Culture from Mouse Bone Marrow under Simulated Microgravity: Lessons for Stromal Lineage Functions
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CUL4B functions as a tumor suppressor in KRAS-driven lung tumors by inhibiting the recruitment of myeloid-derived suppressor cells
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Red cabbage juice-mediated gut microbiota modulation improves intestinal epithelial homeostasis and ameliorates colitis
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Optineurin deficiency impairs autophagy to cause interferon beta overproduction and increased survival of mice following viral infection
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PloS one 2023-06-23
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p53 inhibitor iASPP is an unexpected suppressor of KRAS and inflammation-driven pancreatic cancer
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MPC-n (IgG) improves long-term cognitive impairment in the mouse model of repetitive mild traumatic brain injury
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BMC medicine 2023-05-30
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Tumour extracellular vesicles and particles induce liver metabolic dysfunction
Authors: Wang, G;Li, J;Bojmar, L;Chen, H;Li, Z;Tobias, GC;Hu, M;Homan, EA;Lucotti, S;Zhao, F;Posada, V;Oxley, PR;Cioffi, M;Kim, HS;Wang, H;Lauritzen, P;Boudreau, N;Shi, Z;Burd, CE;Zippin, JH;Lo, JC;Pitt, GS;Hernandez, J;Zambirinis, CP;Hollingsworth, MA;Grandgenett, PM;Jain, M;Batra, SK;DiMaio, DJ;Grem, JL;Klute, KA;Trippett, TM;Egeblad, M;Paul, D;Bromberg, J;Kelsen, D;Rajasekhar, VK;Healey, JH;Matei, IR;Jarnagin, WR;Schwartz, RE;Zhang, H;Lyden, D;
Nature 2023-05-24
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Myeloid Cell Derived IL1? Contributes to Pulmonary Vascular Remodeling in Heart Failure with Preserved Ejection Fraction
Authors: Agrawal, V;Kropski, JA;Gokey, JJ;Kobeck, E;Murphy, M;Murray, KT;Fortune, NL;Moore, CS;Meoli, DF;Monahan, K;Su, YR;Blackwell, T;Gupta, DK;Talati, MH;Gladson, S;Carrier, EJ;West, JD;Hemnes, AR;
bioRxiv : the preprint server for biology 2023-05-18
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Progerinin, an Inhibitor of Progerin, Alleviates Cardiac Abnormalities in a Model Mouse of Hutchinson-Gilford Progeria Syndrome
Authors: Kang, SM;Seo, S;Song, EJ;Kweon, O;Jo, AH;Park, S;Woo, TG;Kim, BH;Oh, GT;Park, BJ;
Cells 2023-04-24
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Irradiation plus myeloid-derived suppressor cell-targeted therapy for overcoming treatment resistance in immunologically cold urothelial carcinoma
Authors: S Yamamoto, M Kato, Y Takeyama, Y Azuma, N Yukimatsu, Y Hirayama, T Otoshi, T Yamasaki, M Fujioka, M Gi, H Wanibuchi, J Uchida
British Journal of Cancer, 2023-04-17;0(0):. 2023-04-17
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Spatiotemporal Cofilin Signaling, Microglial Activation, Neuroinflammation, and Cognitive Impairment Following Hemorrhagic Brain Injury
Authors: Almarghalani, DA;Sha, X;Mrak, RE;Shah, ZA;
Cells 2023-04-13
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Astrocytic Slc4a4 regulates blood-brain barrier integrity in healthy and stroke brains via a NO-CCL2-CCR2 pathway
Authors: Q Ye, J Jo, CY Wang, H Oh, TJ Choy, K Kim, A Dâ Alessan, YK Reshetnyak, SY Jung, Z Chen, SP Marrelli, HK Lee
bioRxiv : the preprint server for biology, 2023-04-03;0(0):. 2023-04-03
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Ionizing Radiation Selectively Increases CXC Ligand 10 Level via the DNA-Damage-Induced p38 MAPK-STAT1 Pathway in Murine J774A.1 Macrophages
Authors: YN Seo, JS Baik, SM Lee, JE Lee, HR Ahn, MS Lim, MT Park, SD Kim
Cells, 2023-03-25;12(7):. 2023-03-25
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Correction of haemorrhagic shock-associated coagulopathy and impaired haemostasis by plasma, prothrombin complex concentrates or an activated protein C-targeted DNA aptamer in mice
Authors: LJ Eltringham, SC Meixner, ELG Pryzdial, WP Sheffield
Scientific Reports, 2023-03-07;13(1):3811. 2023-03-07
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EMBO Mol Med, 2018-07-01;0(0):. 2018-07-01
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Alternaria alternata challenge at the nasal mucosa results in eosinophilic inflammation and increased susceptibility to influenza virus infection
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Clin. Exp. Allergy, 2018-04-15;0(0):. 2018-04-15
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Identification of CCL5/RANTES as a novel contraction-reducible myokine in mouse skeletal muscle
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Cytokine, 2018-03-17;108(0):17-23. 2018-03-17
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Effects of Graphene Oxide Nanoparticles on the Immune System Biomarkers Produced by RAW 264.7 and Human Whole Blood Cell Cultures
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Nanomaterials (Basel), 2018-02-24;8(2):. 2018-02-24
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HEPES activates a MiT/TFE-dependent lysosomal-autophagic gene network in cultured cells: A call for caution
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The role of HFE genotype in macrophage phenotype
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Ultramicronized palmitoylethanolamide rescues learning and memory impairments in a triple transgenic mouse model of Alzheimer's disease by exerting anti-inflammatory and neuroprotective effects
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Neuropharmacology, 2018-01-31;133(0):121-128. 2018-01-31
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Therapeutic effects of telomerase in mice with pulmonary fibrosis induced by damage to the lungs and short telomeres
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A non-cell-autonomous role for Pml in the maintenance of leukemia from the niche
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EphrinA1-Fc attenuates myocardial ischemia/reperfusion injury in mice
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Sox2 Communicates With Tregs Through CCL1 To Promote The Stemness Property Of Breast Cancer Cells
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Stem Cells, 2017-12-01;0(0):. 2017-12-01
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Root bark of Ulmus davidiana var. japonica restrains acute alcohol-induced hepatic steatosis onset in mice by inhibiting ROS accumulation
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PLoS ONE, 2017-11-27;12(11):e0188381. 2017-11-27
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Sci Rep, 2017-10-26;7(1):14110. 2017-10-26
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Co-encapsulation and co-transplantation of mesenchymal stem cells reduces pericapsular fibrosis and improves encapsulated islet survival and function when allografted
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Sci Rep, 2017-08-30;7(1):10059. 2017-08-30
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Umbilical cord-derived mesenchymal stem cell extracts reduce colitis in mice by re-polarizing intestinal macrophages
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Sci Rep, 2017-08-25;7(1):9412. 2017-08-25
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Calf Spleen Extractive Injection protects mice against cyclophosphamide-induced hematopoietic injury through G-CSF-mediated JAK2/STAT3 signaling
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Sci Rep, 2017-08-21;7(1):8402. 2017-08-21
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HDAC3 inhibition ameliorates spinal cord injury by immunomodulation
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Stromal Cell-Derived Factor 1 Mediates Immune Cell Attraction upon Urinary Tract Infection
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Cell Rep, 2017-07-05;20(1):40-47. 2017-07-05
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Rapid monocyte infiltration following retinal detachment is dependent on non-canonical IL6 signaling through gp130
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J Neuroinflammation, 2017-06-23;14(1):121. 2017-06-23
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Loss of epidermal AP1 transcription factor function reduces filaggrin level, alters chemokine expression and produces an ichthyosis-related phenotype
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Recruited Monocytes and Type 2 Immunity Promote Lung Regeneration following Pneumonectomy
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Cell Stem Cell, 2017-05-11;0(0):. 2017-05-11
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Galectin-3: A Positive Regulator of Leukocyte Recruitment in the Inflamed Microcirculation
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Androgen receptor in cancer-associated fibroblasts influences stemness in cancer cells
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Neuronal p38? mediates synaptic and cognitive dysfunction in an Alzheimer's mouse model by controlling ?-amyloid production
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Sci Rep, 2017-03-31;7(0):45306. 2017-03-31
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Diminished bone regeneration after debridement of posttraumatic osteomyelitis is accompanied by altered cytokine levels, elevated B cell activity and increased osteoclast activity
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Inflammatory 'double hit' model of temporomandibular joint disorder with elevated CCL2, CXCL9, CXCL10, RANTES and behavioural hypersensitivity in TNFR1/R2-/- mice
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Eur J Pain, 2017-03-20;0(0):. 2017-03-20
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Effective combinatorial immunotherapy for castration-resistant prostate cancer
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Low-Intensity Training and the C5a Complement Antagonist NOX-D21 Rescue the mdx Phenotype through Modulation of Inflammation
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Am. J. Pathol, 2017-03-18;0(0):. 2017-03-18
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Exosomes function in antigen presentation during an in vivo Mycobacterium tuberculosis infection
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Sci Rep, 2017-03-06;7(0):43578. 2017-03-06
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Cardiac Function Remains Impaired Despite Reversible Cardiac Remodeling after Acute Experimental Viral Myocarditis
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J Immunol Res, 2017-03-02;2017(0):6590609. 2017-03-02
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Osteocyte secreted factors inhibit skeletal muscle differentiation
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Bone Rep, 2017-03-02;6(0):74-80. 2017-03-02
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Importance of CD200 expression by tumor or host cells to regulation of immunotherapy in a mouse breast cancer model
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PLoS ONE, 2017-02-24;12(2):e0171586. 2017-02-24
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Complement drives glucosylceramide accumulation and tissue inflammation in Gaucher disease
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Lack of ABCG2 Leads to Biventricular Dysfunction and Remodeling in Response to Hypoxia
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Front Physiol, 2017-02-21;8(0):98. 2017-02-21
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Fractalkine-CX3CR1 signaling is critical for progesterone-mediated neuroprotection in the retina
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Sci Rep, 2017-02-20;7(0):43067. 2017-02-20
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PD-L1 blockade enhances response of pancreatic ductal adenocarcinoma to radiotherapy
EMBO Mol Med, 2017-02-01;0(0):. 2017-02-01
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DNA damage induced by Strontium-90 exposure at low concentrations in mesenchymal stromal cells: the functional consequences
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Sci Rep, 2017-01-30;7(0):41580. 2017-01-30
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Mitogen-activated Protein Kinase Kinase Activity Maintains Acinar-to-Ductal Metaplasia and Is Required for Organ Regeneration in Pancreatitis
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Oncotarget, 2016-12-13;0(0):. 2016-12-13
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Gold nanoparticle-filled biodegradable photopolymer scaffolds induced muscle remodeling: in vitro and in vivo findings
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Mater Sci Eng C Mater Biol Appl, 2016-12-02;72(0):625-630. 2016-12-02
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INF-? encoding plasmid administration triggers bone loss and disrupts bone marrow microenvironment
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J. Endocrinol, 2016-12-01;0(0):. 2016-12-01
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The cardiac maladaptive ATF3-dependent cross-talk between cardiomyocytes and macrophages is mediated by the IFN?-CXCL10-CXCR3 axis
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Immune response characterization and vaccine potential of a recombinant chimera comprising B-cell epitope of Aeromonas hydrophila outer membrane protein C and LTB
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Vaccine, 2016-11-07;34(50):6259-6266. 2016-11-07
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LAG-3 confers poor prognosis and its blockade reshapes antitumor response in head and neck squamous cell carcinoma
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Oncoimmunology, 2016-10-07;5(11):e1239005. 2016-10-07
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Role of the B Allele of Influenza A Virus Segment 8 in Setting Mammalian Host Range and Pathogenicity
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J. Virol., 2016-09-29;90(20):9263-84. 2016-09-29
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Inflammatory monocytes and NK cells play a crucial role in DNAM-1-dependent control of cytomegalovirus infection
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The Thyroid Hormone Receptors Inhibit Hepatic Interleukin-6 Signaling During Endotoxemia
Sci Rep, 2016-08-03;6(0):30990. 2016-08-03
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The Anti-Inflammatory Effects and Mechanisms of Eupafolin in Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Macrophages
PLoS ONE, 2016-07-14;11(7):e0158662. 2016-07-14
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Treatment of refractory cutaneous ulcers with mixed sheets consisting of peripheral blood mononuclear cells and fibroblasts
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Sci Rep, 2016-06-22;6(0):28538. 2016-06-22
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Proatherosclerotic Effect of the ?1-Subunit of Soluble Guanylyl Cyclase by Promoting Smooth Muscle Phenotypic Switching
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Am J Pathol, 2016-06-15;0(0):. 2016-06-15
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Impact of the Sensory Neurons on Melanoma Growth In Vivo
PLoS ONE, 2016-05-26;11(5):e0156095. 2016-05-26
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Targeting Serglycin Prevents Metastasis in Murine Mammary Carcinoma
PLoS ONE, 2016-05-25;11(5):e0156151. 2016-05-25
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Neutrophils suppress intraluminal NK-mediated tumor cell clearance and enhance extravasation of disseminated carcinoma cells
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PLoS ONE, 2016-01-26;11(1):e0147710. 2016-01-26
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Extracellular Histones Induce Chemokine Production in Whole Blood Ex Vivo and Leukocyte Recruitment In Vivo.
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Left Ventricular Dysfunction and CXCR3 Ligands in Hypertension: From Animal Experiments to a Population-Based Pilot Study.
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Novel Role of TRPML2 in the Regulation of the Innate Immune Response.
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Shikonin-enhanced cell immunogenicity of tumor vaccine is mediated by the differential effects of DAMP components.
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Mol Cancer, 2015-09-24;14(0):174. 2015-09-24
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Plasmacytoid Dendritic Cell Activation and IFN-alpha Production Are Prominent Features of Murine Autoimmune Pancreatitis and Human IgG4-Related Autoimmune Pancreatitis.
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Snail1-induced partial epithelial-to-mesenchymal transition drives renal fibrosis in mice and can be targeted to reverse established disease.
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Molecular Mechanisms Mediating Retinal Reactive Gliosis Following Bone Marrow Mesenchymal Stem Cell Transplantation.
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EpCAM Aptamer-siRNA Chimera Targets and Regress Epithelial Cancer.
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mRNA-transfected Dendritic Cells Expressing Polypeptides That Link MHC-I Presentation to Constitutive TLR4 Activation Confer Tumor Immunity.
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Combination of inflammation-related cytokines promotes long-term muscle stem cell expansion.
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Cell Res, 2015-05-15;25(6):655-73. 2015-05-15
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Lenalidomide reduces microglial activation and behavioral deficits in a transgenic model of Parkinson's disease.
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MicroRNA-26a/-26b-COX-2-MIP-2 Loop Regulates Allergic Inflammation and Allergic Inflammation-promoted Enhanced Tumorigenic and Metastatic Potential of Cancer Cells.
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Grapefruit-Derived Nanovectors Use an Activated Leukocyte Trafficking Pathway to Deliver Therapeutic Agents to Inflammatory Tumor Sites.
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Tumor-induced pressure in the bone microenvironment causes osteocytes to promote the growth of prostate cancer bone metastases.
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Specific medicinal plant polysaccharides effectively enhance the potency of a DC-based vaccine against mouse mammary tumor metastasis.
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Virus binding to a plasma membrane receptor triggers interleukin-1 alpha-mediated proinflammatory macrophage response in vivo.
Authors: Di Paolo NC, Miao EA, Iwakura Y, Murali-Krishna K, Aderem A, Flavell RA, Papayannopoulou T, Shayakhmetov DM
Immunity, 2009-07-02;31(1):110-21. 2009-07-02
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JNK-induced MCP-1 production in spinal cord astrocytes contributes to central sensitization and neuropathic pain.
Authors: Gao YJ, Zhang L, Samad OA, Suter MR, Yasuhiko K, Xu ZZ, Park JY, Lind AL, Ma Q, Ji RR
J. Neurosci., 2009-04-01;29(13):4096-108. 2009-04-01
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Inhibition of the chemokine (C-C motif) ligand 2/chemokine (C-C motif) receptor 2 pathway attenuates hyperglycaemia and inflammation in a mouse model of hepatic steatosis and lipoatrophy.
Authors: Yang SJ, IglayReger HB, Kadouh HC, Bodary PF
Diabetologia, 2009-03-10;52(0):972-81. 2009-03-10
FAQs
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Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?
The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.
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