Summary
Precision
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Mouse CCL12/MCP-5 Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL)
Sensitivity
2.1 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant mouse MCP-5
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse MCP-5 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse MCP-5 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse MCP-5 and antibodies raised against the recombinant factor. Results obtained for naturally occurring mouse MCP-5 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse MCP-5.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 32 | 85 | 318 | 31 | 86 | 318 |
| Standard Deviation | 1.8 | 3.5 | 18 | 2.8 | 7.9 | 33 |
| CV% | 5.6 | 4.1 | 5.7 | 9 | 9.2 | 10.4 |
Recovery
The recovery of mouse MCP-5 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=6) | 94 | 81-119 |
| EDTA Plasma (n=6) | 99 | 84-114 |
| Serum (n=6) | 93 | 80-108 |
Linearity
To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of mouse MCP-5 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data
Product Datasheets
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL12/MCP-5
Mouse MCP-5, also known as CCL12, encodes a 104 amino acid (aa) precursor protein with a 22 aa hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. MCP-5 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, MCP-5 expression is also induced in activated macrophages and mast cells.
Entrez Gene IDs:
20293 (Mouse)
Alternate Names:
C-C motif chemokine 12; CCL12; chemokine (C-C motif) ligand 12; MCP5; MCP-5; Scya12
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Citations for Mouse CCL12/MCP-5 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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LHPP-mediated inorganic pyrophosphate hydrolysis-driven lysosomal acidification in astrocytes regulates adult neurogenesis
Authors: Sha, L;Li, J;Shen, H;Wang, Q;Meng, P;Zhang, X;Deng, Y;Zhu, W;Xu, Q;
Cell reports
Species: Mouse
Sample Types: Cell Culture Supernates
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Biomimetic nanoparticles deliver mRNAs encoding costimulatory receptors and enhance T cell mediated cancer immunotherapy
Authors: W Li, X Zhang, C Zhang, J Yan, X Hou, S Du, C Zeng, W Zhao, B Deng, DW McComb, Y Zhang, DD Kang, J Li, WE Carson, Y Dong
Nature Communications, 2021-12-14;12(1):7264.
Species: Mouse
Sample Types: Plasma
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Varying susceptibility of pulmonary cytokine production to lipopolysaccharide in mice.
Authors: Alm AS, Li K, Yang D, Andersson R, Lu Y, Wang X
Cytokine, 2009-12-29;49(3):256-63.
Species: Mouse
Sample Types: BALF
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Src homology 2 domain-containing inositol 5-phosphatase 1 deficiency leads to a spontaneous allergic inflammation in the murine lung.
Authors: Oh SY, Zheng T, Bailey ML, Barber DL, Schroeder JT, Kim YK, Zhu Z
J. Allergy Clin. Immunol., 2006-10-23;119(1):123-31.
Species: Mouse
Sample Types: BALF
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Type I IFN modulates host defense and late hyperinflammation in septic peritonitis.
Authors: Weighardt H, Kaiser-Moore S, Schlautkotter S, Rossmann-Bloeck T, Schleicher U, Bogdan C, Holzmann B
J. Immunol., 2006-10-15;177(8):5623-30.
Species: Mouse
Sample Types: Peritoneal Lavage Fluid
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Nuclear factor-kappaB affects tumor progression in a mouse model of malignant pleural effusion.
Authors: Stathopoulos GT, Zhu Z, Everhart MB, Kalomenidis I, Lawson WE, Bilaceroglu S, Peterson TE, Mitchell D, Yull FE, Light RW, Blackwell TS
Am. J. Respir. Cell Mol. Biol., 2005-10-06;34(2):142-50.
Species: Mouse
Sample Types: Serum
FAQs
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Mouse CCL12/MCP-5 Quantikine ELISA Kit
By Rahul Kumar
on 05/19/2017
Sample Tested: mouse lung tissue
