Home / Rho-kinase Inhibitors / Y-27632 dihydrochloride

Y-27632 dihydrochloride

Catalog #: 1254
252 Citations 4 Reviews Datasheet
GMP Version Available: TB1254-GMP
GMP
Catalog # Availability Size / Price Qty
1254/10
1254/50
1254/1
Y-27632 dihydrochloride | CAS No. 129830-38-2 | Rho-kinase Inhibitors
2 Images
Description: Selective ROCK inhibitor; inhibits ROCK1 (p160 ROCK) and ROCK2; stem cell freezing (cryopreservation) media component
Alternative Names: y27632,Y-27632 2HCl,ROCK inhibitor,ROCK1 inhibitor

Chemical Name: trans-4-[(1R)-1-Aminoethyl]-N-4-pyridinylcyclohexanecarboxamide dihydrochloride

Purity: ≥98%

Product Details
Citations (252)
Supplemental Products
Reviews (4)
Biological Activity Scientific Data Technical Data Background Product Datasheets Calculators

Biological Activity

Y-27632 dihydrochloride is a selective ROCK inhibitor (Ki values are 0.14-0.22, 0.3, 25, 26 and > 250 μM for ROCK1 (p160 ROCK), ROCK2, PKA, PKC and MLCK respectively). Also inhibits PRK2 (IC50 = 600 nM). Increases survival rate of human embryonic stem (hES) cells and iPSC undergoing cryopreservation. Used in combination with CHIR 99021, RepSox, Forskolin, SP 600125, Go 6983 and VPA to reprogram fibroblasts to mature neurons. Also optimizes naive human pluripotent stem cell growth and viability following naive cell derivation from primed ESCs and iPSCs using naive human stem cell medium (NHSM). Used as component of growth media for urothelial organoids. Also used in a protocol to generate brain organoids from human iPSCs. Cell-permeable, orally bioavailable and active in vivo.

Y-27632 in solution and Y-27632 synthesized to cGMP guidelines also available.

For more information about how Y-27632 dihydrochloride may be used, see our protocols: Transdifferentiating Fibroblasts into Neurons, Converting Fibroblasts into Cardiomyocytes (9C Cocktail), Accelerated Induction of Cortical Neurons from hiPSCs, Human iPSC-derived Myoblasts.

Scientific Data

N/A ROCK inhibition by Y-27632 and immunostaining of skeletal myogenic differentiation of MESP1+ mesoderm, View Larger

Application of Y-27632 in MESP1+ Mesoderm. ROCK inhibition by Y-27632 (1 μM) promotes skeletal myogenic differentiation of MESP1+ mesoderm, as shown by immunostaining for Myosin Heavy Chain+ (Green) and Myogenin (Red), and counterstaining with DAPI (Catalog # 5748; Blue, Tocris). Data courtesy of Dr Sunny Chan and Dr Michael Kyba, University of Minnesota.

Technical Data

M.Wt:
320.26
Formula:
C14H21N3O.2HCl
Solubility:
Soluble to 100 mM in water and to 100 mM in DMSO
Purity:
≥98%
Storage:
Desiccate at RT
CAS No:
129830-38-2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. Derivation of novel human ground state naive pluripotent stem cells.
    Gafni O, Weinberger L, Mansour AA et al.
    Nature
  2. Human Pluripotent Stem Cell-Derived Atrial and Ventricular Cardiomyocytes Develop from Distinct Mesoderm Populations
    JH Lee, SI Protze, Z Laksman, PH Backx, GM Keller
    Cell Stem Cell, 2017;21(2):179-194.e4.
  3. Efficient generation of human primordial germ cell-like cells from pluripotent stem cells in a methylcellulose-based 3D system at large scale
    X Wang, T Liao, C Wan, X Yang, J Zhao, R Fu, Z Yao, Y Huang, Y Shi, G Chang, Y Zheng, F Luo, Z Liu, Y Wang, X Mao, XY Zhao
    PeerJ, 2019;6(0):e6143.
  4. Neurokinin 1 receptor mediates membrane blebbing and sheer stress-induced microparticle formation in HEK293 cells.
    Chen, Panpan, Douglas, Steven D, Meshki, John, Tuluc, Florin
    PLoS ONE, 2012;7(9):e45322.
  5. A Multiwell Cardiac ?GMEA Platform for Action Potential Recordings from Human iPSC-Derived Cardiomyocyte Constructs
    SL Edwards, V Zlochiver, DB Conrad, R Vaidyanath, AM Valiquette, R Joshi-Mukh
    Stem Cell Reports, 2018;0(0):.
  6. Mammary-Stem-Cell-Based Somatic Mouse Models Reveal Breast Cancer Drivers Causing Cell Fate Dysregulation

    Cell Rep, 2016;16(12):3146-56.
  7. 3D Modeling of Esophageal Development using Human PSC-Derived Basal Progenitors Reveals a Critical Role for Notch Signaling
    Y Zhang, Y Yang, M Jiang, SX Huang, W Zhang, D Al Alam, S Danopoulos, M Mori, YW Chen, R Balasubram, SM Chuva de S, C Serra, M Bialecka, E Kim, S Lin, ALR Toste de C, PN Riccio, WV Cardoso, X Zhang, HW Snoeck, J Que
    Cell Stem Cell, 2018;0(0):.
  8. Signals from the surface modulate differentiation of human pluripotent stem cells through glycosaminoglycans and integrins.
    Wrighton, Paul J, Klim, Joseph R, Hernandez, Brandon, Koonce, Chad H, Kamp, Timothy, Kiessling, Laura L
    Proc Natl Acad Sci U S A, 2014;111(51):18126-31.
  9. Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors
    KF Cogger, A Sinha, F Sarangi, EC McGaugh, D Saunders, C Dorrell, S Mejia-Guer, Y Aghazadeh, JL Rourke, RA Screaton, M Grompe, PR Streeter, AC Powers, M Brissova, T Kislinger, MC Nostro
    Nat Commun, 2017;8(1):331.
  10. Establishment of an Immortalized Skin Keratinocyte Cell Line Derived from the Animal Model Mastomys coucha

    PLoS ONE, 2016;11(8):e0161283.
  11. The P2X7 receptor is an upstream regulator of dynamic blebbing and a pluripotency marker in human embryonic stem cells
    NJ Weng, P Talbot
    Stem Cell Res, 2017;23(0):39-49.
  12. Release of cellular tension signals self-restorative ventral lamellipodia to heal barrier micro-wounds.
    Martinelli, Roberta, Kamei, Masataka, Sage, Peter T, Massol, Ramiro, Varghese, Laya, Sciuto, Tracey, Toporsian, Mourad, Dvorak, Ann M, Kirchhausen, Tomas, Springer, Timothy, Carman, Christop
    J Cell Biol, 2013;201(3):449-65.
  13. Stem Cell Antigen-1 Identifies a Distinct Androgen-Independent Murine Prostatic Luminal Cell Lineage with Bipotent Potential.
    Kwon O, Zhang L, Xin L
    Stem Cells, 2016;34(1):191-202.
  14. Deterministic HOX patterning in human pluripotent stem cell-derived neuroectoderm

    Stem Cell Reports, 2015;4(4):632-44.
  15. Chromatin structure of two genomic sites for targeted transgene integration in induced pluripotent stem cells and hematopoietic stem cells.
    van Rensburg, R, Beyer, I, Yao, X-Y, Wang, H, Denisenko, O, Li, Z-Y, Russell, D W, Miller, D G, Gregory, P, Holmes, M, Bomsztyk, K, Lieber, A
    Gene Ther, 2013;20(2):201-14.
  16. Oligomeric proanthocyanidins (OPCs) target cancer stem-like cells and suppress tumor organoid formation in colorectal cancer
    S Toden, P Ravindrana, J Gu, J Cardenas, M Yuchang, A Goel
    Sci Rep, 2018;8(1):3335.
  17. A combination of curcumin and oligomeric proanthocyanidins offer superior anti-tumorigenic properties in colorectal cancer
    P Ravindrana, D Pasham, U Balaji, J Cardenas, J Gu, S Toden, A Goel
    Sci Rep, 2018;8(1):13869.
  18. Atypical PKC and Notch Inhibition Differentially Modulate Cortical Interneuron Subclass Fate from Embryonic Stem Cells
    DJ Tischfield, J Kim, SA Anderson
    Stem Cell Reports, 2017;0(0):.
  19. MeCP2-regulated miRNAs control early human neurogenesis through differential effects on ERK and AKT signaling
    N Mellios, DA Feldman, SD Sheridan, JPK Ip, S Kwok, SK Amoah, B Rosen, BA Rodriguez, B Crawford, R Swaminatha, S Chou, Y Li, M Ziats, C Ernst, R Jaenisch, SJ Haggarty, M Sur
    Mol. Psychiatry, 2017;0(0):.
  20. Dynamic blebbing: A bottleneck to human embryonic stem cell culture that can be overcome by Laminin-Integrin signaling
    NJ Weng, C Cheung, P Talbot
    Stem Cell Res, 2018;33(0):233-246.
  21. Development of a primary mouse intestinal epithelial cell monolayer culture system to evaluate factors that modulate IgA transcytosis.
    Moon C, VanDussen K, Miyoshi H, Stappenbeck T
    Mucosal Immunol, 2014;7(4):818-28.
  22. TALEN/CRISPR-mediated eGFP knock-in add-on at the OCT4 locus does not impact differentiation of human embryonic stem cells towards endoderm.
    Krentz, Nicole A, Nian, Cuilan, Lynn, Francis
    PLoS ONE, 2014;9(12):e114275.
  23. Pharmaceutical iron formulations do not cross a model of the human blood-brain barrier
    B Chiou, EH Neal, AB Bowman, ES Lippmann, IA Simpson, JR Connor
    PLoS ONE, 2018;13(6):e0198775.
  24. Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA
    MA Scavuzzo, J Chmielowie, D Yang, K Wamble, LS Chaboub, L Duraine, B Tepe, SM Glasgow, BR Arenkiel, C Brou, B Deneen, M Borowiak
    Cell Rep, 2018;25(13):3811-3827.e7.
  25. TMEM258 Is a Component of the Oligosaccharyltransferase Complex Controlling ER Stress and Intestinal Inflammation

    Cell Rep, 2016;17(11):2955-2965.
  26. Deterministic HOX Patterning in Human Pluripotent Stem Cell-Derived Neuroectoderm.
    Lippmann E, Williams C, Ruhl D, Estevez-Silva M, Chapman E, Coon J, Ashton R
    Stem Cell Reports, 2015;4(4):632-44.
  27. VE-Cadherin Disassembly and Cell Contractility in the Endothelium are Necessary for Barrier Disruption Induced by Tumor Cells
    V Aragon-San, SE Pohler, VJ Eswar, M Bierowski, EW Gomez, C Dong
    Sci Rep, 2017;7(0):45835.
  28. GATA2/3-TFAP2A/C transcription factor network couples human pluripotent stem cell differentiation to trophectoderm with repression of pluripotency
    C Krendl, D Shaposhnik, V Rishko, C Ori, C Ziegenhain, S Sass, L Simon, NS Müller, T Straub, KE Brooks, SL Chavez, W Enard, FJ Theis, M Drukker
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
  29. Three-Dimensional Organoids Reveal Therapy Resistance of Esophageal and Oropharyngeal Squamous Cell Carcinoma Cells
    T Kijima, H Nakagawa, M Shimonoson, PM Chandramou, T Hara, V Sahu, Y Kasagi, O Kikuchi, K Tanaka, V Giroux, AB Muir, KA Whelan, S Ohashi, S Naganuma, AJ Klein-Szan, Y Shinden, K Sasaki, I Omoto, Y Kita, M Muto, AJ Bass, JA Diehl, GG Ginsberg, Y Doki, M Mori, Y Uchikado, T Arigami, NG Avadhani, D Basu, AK Rustgi, S Natsugoe
    Cell Mol Gastroenterol Hepatol, 2019;7(1):73-91.
  30. Cell adhesion and shape regulate TGF-beta1-induced epithelial-myofibroblast transition via MRTF-A signaling.
    O'Connor J, Gomez E
    PLoS ONE, 2013;8(12):e83188.
  31. Principles of early human development and germ cell program from conserved model systems
    T Kobayashi, H Zhang, WWC Tang, N Irie, S Withey, D Klisch, A Sybirna, S Dietmann, DA Contreras, R Webb, C Allegrucci, R Alberio, MA Surani
    Nature, 2017;546(7658):416-420.
  32. Xeno- and feeder-free differentiation of human pluripotent stem cells to two distinct ocular epithelial cell types using simple modifications of one method
    H Hongisto, T Ilmarinen, M Vattulaine, A Mikhailova, H Skottman
    Stem Cell Res Ther, 2017;8(1):291.
  33. Harnessing single-cell genomics to improve the physiological fidelity of organoid-derived cell types
    BE Mead, J Ordovas-Mo, AP Braun, LE Levy, P Bhargava, MJ Szucs, DA Ammendolia, MA MacMullan, X Yin, TK Hughes, MH Wadsworth, R Ahmad, S Rakoff-Nah, SA Carr, R Langer, JJ Collins, AK Shalek, JM Karp
    BMC Biol., 2018;16(1):62.
  34. Prospective isolation of NKX2-1-expressing human lung progenitors derived from pluripotent stem cells
    F Hawkins, P Kramer, A Jacob, I Driver, DC Thomas, KB McCauley, N Skvir, AM Crane, AA Kurmann, AN Hollenberg, S Nguyen, BG Wong, AS Khalil, SX Huang, S Guttentag, JR Rock, JM Shannon, BR Davis, DN Kotton
    J. Clin. Invest., 2017;127(6):2277-2294.
  35. Efficient derivation of sympathetic neurons from human pluripotent stem cells with a defined condition
    K Kirino, T Nakahata, T Taguchi, MK Saito
    Sci Rep, 2018;8(1):12865.
  36. Stage-Specific Human Induced Pluripotent Stem Cells Map the Progression of Myeloid Transformation to Transplantable Leukemia
    AG Kotini, CJ Chang, A Chow, H Yuan, TC Ho, T Wang, S Vora, A Solovyov, C Husser, M Olszewska, J Teruya-Fel, D Perumal, VM Klimek, A Spyridonid, RK Rampal, L Silverman, EP Reddy, E Papaemmanu, S Parekh, BD Greenbaum, CS Leslie, MG Kharas, EP Papapetrou
    Cell Stem Cell, 2017;0(0):.
  37. Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes
    GB Ferguson, B Van Handel, M Bay, P Fiziev, T Org, S Lee, R Shkhyan, NW Banks, M Scheinberg, L Wu, B Saitta, J Elphingsto, AN Larson, SM Riester, AD Pyle, NM Bernthal, HK Mikkola, J Ernst, AJ van Wijnen, M Bonaguidi, D Evseenko
    Nat Commun, 2018;9(1):3634.
  38. Open Chromatin Profiling in hiPSC-Derived Neurons Prioritizes Functional Noncoding Psychiatric Risk Variants and Highlights Neurodevelopmental Loci
    MP Forrest, H Zhang, W Moy, H McGowan, C Leites, LE Dionisio, Z Xu, J Shi, AR Sanders, WJ Greenleaf, CA Cowan, ZP Pang, PV Gejman, P Penzes, J Duan
    Cell Stem Cell, 2017;0(0):.
  39. ZFX controls the self-renewal of human embryonic stem cells.
    Harel, Sivan, Tu, Edmund Y, Weisberg, Stuart, Esquilin, Manuel, Chambers, Stuart M, Liu, Becky, Carson, Christia, Studer, Lorenz, Reizis, Boris, Tomishima, Mark J
    PLoS ONE, 2012;7(8):e42302.
  40. Defined human pluripotent stem cell culture enables highly efficient neuroepithelium derivation without small molecule inhibitors.
    Lippmann E, Estevez-Silva M, Ashton R
    Stem Cells, 2014;32(4):1032-42.
  41. Nephron organoids derived from human pluripotent stem cells model kidney development and injury.
    Morizane R, Lam A, Freedman B, Kishi S, Valerius M, Bonventre J
    Nat Biotechnol, 2015;33(11):1193-200.
  42. Purinergic P2Y2 Receptor Control of Tissue Factor Transcription in Human Coronary Artery Endothelial Cells: NEW AP-1 TRANSCRIPTION FACTOR SITE AND NEGATIVE REGULATOR.
    Liu Y, Zhang L, Wang C, Roy S, Shen J
    J Biol Chem, 2016;291(4):1553-63.
  43. Increased susceptibility to a? toxicity in neuronal cultures derived from familial Azheimer's disease (PSEN1-A246E) induced pluripotent stem cells
    Enrique Armijo
    Neurosci. Lett, 2016;0(0):.
  44. TGF-beta-activated kinase 1 (TAK1) signaling regulates TGF-beta-induced WNT-5A expression in airway smooth muscle cells via Sp1 and beta-catenin.
    Kumawat K, Menzen M, Slegtenhorst R, Halayko A, Schmidt M, Gosens R
    PLoS ONE, 2014;9(4):e94801.
  45. Transcriptionally and Functionally Distinct Mesenchymal Subpopulations Are Generated from Human Pluripotent Stem Cells
    CJ Chin, S Li, M Corselli, D Casero, Y Zhu, CB He, R Hardy, B Péault, GM Crooks
    Stem Cell Reports, 2018;0(0):.
  46. Expression of human decay-accelerating factor on intestinal epithelium of transgenic mice does not facilitate infection by the enteral route.
    Pan J, Zhang L, Odenwald M, Shen L, Turner J, Bergelson J
    J Virol, 2015;89(8):4311-8.
  47. Generation of Spinocerebellar Ataxia Type 2 induced pluripotent stem cell lines, CHOPi002-A and CHOPi003-A, from patients with abnormal CAG repeats in the coding region of the ATXN2 gene
    JA Maguire, AL Gagne, P Gonzalez-A, BL Davidson, V Shakkottai, P Gadue, DL French
    Stem Cell Res, 2018;34(0):101361.
  48. Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells
    AJ Alshawaf, S Viventi, W Qiu, G D'Abaco, B Nayagam, M Erlichster, G Chana, I Everall, J Ivanusic, E Skafidas, M Dottori
    Sci Rep, 2018;8(1):603.
  49. Altered neuronal network and rescue in a human MECP2 duplication model

    Mol. Psychiatry, 2016;21(2):178-88.
  50. Endothelial-mesenchymal transition of brain endothelial cells: possible role during metastatic extravasation.
    Krizbai I, Gasparics A, Nagyoszi P, Fazakas C, Molnar J, Wilhelm I, Bencs R, Rosivall L, Sebe A
    PLoS ONE, 2015;10(3):e0119655.
  51. Dynamic behaviour of human neuroepithelial cells in the developing forebrain
    L Subramania, M Bershteyn, MF Paredes, AR Kriegstein
    Nat Commun, 2017;8(0):14167.
  52. Generation of skeletal myogenic progenitors from human pluripotent stem cells using non-viral delivery of minicircle DNA
    J Kim, VKP Oliveira, A Yamamoto, RCR Perlingeir
    Stem Cell Res, 2017;23(0):87-94.
  53. Evaluating S. flexneri pathogenesis in the human enteroid model
    S Ranganatha, M Doucet, CL Grassel, B Delaine-El, NC Zachos, EM Barry
    Infect. Immun., 2019;0(0):.
  54. Stable enhanced green fluorescent protein expression after differentiation and transplantation of reporter human induced pluripotent stem cells generated by AAVS1 transcription activator-like effector nucleases.
    Luo Y, Liu C, Cerbini T, San H, Lin Y, Chen G, Rao M, Zou J
    Stem Cells Transl Med, 2014;3(7):821-35.
  55. Derivation and expansion of PAX7-positive muscle progenitors from human and mouse embryonic stem cells.
    Shelton M, Metz J, Liu J, Carpenedo R, Demers S, Stanford W, Skerjanc I
    Stem Cell Reports, 2014;3(3):516-29.
  56. Effects of lung and airway epithelial maturation cocktail on the structure of lung bud organoids
    E Magro-Lope, C Palmer, J Manso, I Liste, A Zambrano
    Stem Cell Res Ther, 2018;9(1):186.
  57. BVES is required for maintenance of colonic epithelial integrity in experimental colitis by modifying intestinal permeability
    YA Choksi, VK Reddy, K Singh, CW Barrett, SP Short, B Parang, CE Keating, JJ Thompson, TG Verriere, RE Brown, MB Piazuelo, DM Bader, MK Washington, MK Mittal, T Brand, AP Gobert, LA Coburn, KT Wilson, CS Williams
    Mucosal Immunol, 2018;0(0):.
  58. Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.
    Edri, Reuven, Yaffe, Yakey, Ziller, Michael, Mutukula, Naresh, Volkman, Rotem, David, Eyal, Jacob-Hirsch, Jasmine, Malcov, Hagar, Levy, Carmit, Rechavi, Gideon, Gat-Viks, Irit, Meissner, Alexande, Elkabetz, Yechiel
    Nat Commun, 2015;6(0):6500.
  59. Capturing the biology of disease severity in a PSC-based model of familial dysautonomia
    N Zeltner, F Fattahi, NC Dubois, N Saurat, F Lafaille, L Shang, B Zimmer, J Tchieu, MA Soliman, G Lee, JL Casanova, L Studer
    Nat. Med., 2016;22(12):1421-1427.
  60. Sinoatrial node cardiomyocytes derived from human pluripotent cells function as a biological pacemaker

    Nat. Biotechnol, 2016;0(0):.
  61. Directed differentiation and functional maturation of cortical interneurons from human embryonic stem cells.
    Maroof A, Keros S, Tyson J, Ying S, Ganat Y, Merkle F, Liu B, Goulburn A, Stanley E, Elefanty A, Widmer H, Eggan K, Goldstein P, Anderson S, Studer L
    Cell Stem Cell, 2013;12(5):559-72.
  62. Caspase-3 dependent nitrergic neuronal apoptosis following cavernous nerve injury is mediated via RhoA and ROCK activation in major pelvic ganglion

    Sci Rep, 2016;6(0):29416.
  63. Single-Cell Sequencing of iPSC-Dopamine Neurons Reconstructs Disease Progression and Identifies HDAC4 as a Regulator of Parkinson Cell Phenotypes
    C Lang, KR Campbell, BJ Ryan, P Carling, M Attar, J Vowles, OV Perestenko, R Bowden, F Baig, M Kasten, MT Hu, SA Cowley, C Webber, R Wade-Marti
    Cell Stem Cell, 2018;0(0):.
  64. Vertically transmitted faecal IgA levels determine extra-chromosomal phenotypic variation.
    Moon C, Baldridge M, Wallace M, Burnham C, Virgin H, Stappenbeck T
    Nature, 2015;521(7550):90-3.
  65. Shaping organs by a wingless-int/Notch/nonmuscle myosin module which orients feather bud elongation.
    Li A, Chen M, Jiang T, Wu P, Nie Q, Widelitz R, Chuong C
    Proc Natl Acad Sci U S A, 2013;110(16):E1452-61.
  66. Efficient generation of lung and airway epithelial cells from human pluripotent stem cells.
    Huang, Sarah X, Islam, Mohammad, O'Neill, John, Hu, Zheng, Yang, Yong-Gua, Chen, Ya-Wen, Mumau, Melanie, Green, Michael, Vunjak-Novakovic, Gordana, Bhattacharya, Jahar, Snoeck, Hans-Wil
    Nat Biotechnol, 2014;32(1):84-91.
  67. Physiological characterisation of human iPS-derived dopaminergic neurons.
    Hartfield, Elizabet, Yamasaki-Mann, Michiko, Ribeiro Fernandes, Hugo J, Vowles, Jane, James, William, Cowley, Sally A, Wade-Martins, Richard
    PLoS ONE, 2014;9(2):e87388.
  68. Efficient Derivation of Functional Human Airway Epithelium from Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling
    KB McCauley, F Hawkins, M Serra, DC Thomas, A Jacob, DN Kotton
    Cell Stem Cell, 2017;0(0):.
  69. Diverse matrix metalloproteinase functions regulate cancer amoeboid migration.
    Orgaz J, Pandya P, Dalmeida R, Karagiannis P, Sanchez-Laorden B, Viros A, Albrengues J, Nestle F, Ridley A, Gaggioli C, Marais R, Karagiannis S, Sanz-Moreno V
    Nat Commun, 2014;5(0):4255.
  70. A New Glucocerebrosidase Chaperone Reduces ?-Synuclein and Glycolipid Levels in iPSC-Derived Dopaminergic Neurons from Patients with Gaucher Disease and Parkinsonism

    J Neurosci, 2016;36(28):7441-52.
  71. A pluripotent stem cell-based model for post-implantation human amniotic sac development
    Y Shao, K Taniguchi, RF Townshend, T Miki, DL Gumucio, J Fu
    Nat Commun, 2017;8(1):208.
  72. Identification of Aging-Associated Gene Expression Signatures That Precede Intestinal Tumorigenesis

    PLoS ONE, 2016;11(9):e0162300.
  73. Effect of densely ionizing radiation on cardiomyocyte differentiation from human-induced pluripotent stem cells
    E Baljinnyam, S Venkatesh, R Gordan, S Mareedu, J Zhang, LH Xie, EI Azzam, CK Suzuki, D Fraidenrai
    Physiol Rep, 2017;5(15):.
  74. Differentiation of Human Pluripotent Stem Cells into Colonic Organoids via Transient Activation of BMP Signaling
    JO Múnera, N Sundaram, SA Rankin, D Hill, C Watson, M Mahe, JE Vallance, NF Shroyer, KL Sinagoga, A Zarzoso-La, JR Hudson, JC Howell, P Chatuvedi, JR Spence, JM Shannon, AM Zorn, MA Helmrath, JM Wells
    Cell Stem Cell, 2017;0(0):.
  75. Protective role of cellular prion protein against TNF?-mediated inflammation through TACE ?-secretase
    J Ezpeleta, F Boudet-Dev, M Pietri, A Baudry, V Baudouin, A Alleaume-B, N Dagoneau, O Kellermann, JM Launay, B Schneider
    Sci Rep, 2017;7(1):7671.
  76. Development of immunocompatible pluripotent stem cells via CRISPR-based human leukocyte antigen engineering
    Y Jang, J Choi, N Park, J Kang, M Kim, Y Kim, JH Ju
    Exp. Mol. Med., 2019;51(1):3.
  77. High-throughput genotoxicity assay identifies antioxidants as inducers of DNA damage response and cell death.
    Fox JT, Sakamuru S, Huang R
    Proc. Natl. Acad. Sci. U.S.A., 2012;109(14):5423-8.
  78. Quiescent innate response to infective filariae by human Langerhans cells suggests a strategy of immune evasion.
    Boyd A, Bennuru S, Wang Y, Sanprasert V, Law M, Chaussabel D, Nutman T, Semnani R
    Infect Immun, 2013;81(5):1420-9.
  79. Cryopreservation of brain endothelial cells derived from human induced pluripotent stem cells is enhanced by Rho-associated coiled coil-containing kinase inhibition.
    Wilson et al.
    Tissue.Eng.Part C.Methods, 2016;22:1085
  80. Development of bipotent cardiac/skeletal myogenic progenitors from MESP1+ mesoderm.
    Chan et al.
    Stem Cell Reports, 2016;6:26
  81. Freeze-thawing single human embryonic stem cells induce e-cadherin and actin filament network disruption via g13 signaling.
    Ichikawa et al.
    Cryo Letters., 2011;32:516
  82. Use and properties of ROCK-specific inhibitor Y-27632.
    Narumiya et al.
    Methods Enzymol., 2000;325:273
  83. Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase.
    Uehata et al.
    Nature, 1997;389:990
  84. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells
    A Montel-Hag, CS Seet, S Li, B Chick, Y Zhu, P Chang, S Tsai, V Sun, S Lopez, HC Chen, C He, CJ Chin, D Casero, GM Crooks
    Cell Stem Cell, 2019;0(0):.
  85. Enhanced Utilization of Induced Pluripotent Stem Cell-Derived Human Intestinal Organoids Using Microengineered Chips.
    Michael J Workman, John P Gleeson, Elissa J Troisi, Hannah Q Estrada, S Jordan Kerns, Christopher D Hinojosa, Geraldine A Hamilton, Stephan R Targan, Clive N Svendsen, Robert J Barrett
    Cellular and Molecular Gastroenterology and Hepatology, 2018;0(0):2352-345X.
  86. In vitro expansion of human gastric epithelial stem cells and their responses to bacterial infection.
    Bartfeld et al.
    Gastroenterology, 2015;148:126
  87. Generation of Cerebral Organoids from Human Pluripotent Stem Cells
    Lancaster et al.
    Nat.Protoc., 2015;9:2329
  88. SnapShot: Growing Organoids from Stem Cells.
    Sato et al.
    Cell, 2015;161:1700
  89. Establishment of gastrointestinal epithelial organoids
    Mahe et al.
    Curr.Protoc. Mouse Biol., 2014;3:217
  90. Human blood vessel organoids as a model of diabetic vasculopathy.
    Wimmer et al.
    Nature, 2019;565:505
  91. Isolation and in vitro expansion of human colonic stem cells.
    Jung et al.
    Nat.Med., 2011;17:1227
  92. Highly Efficient and Marker-free Genome Editing of Human Pluripotent Stem Cells by CRISPR-Cas9 RNP and AAV6 Donor-Mediated Homologous Recombination
    RM Martin, K Ikeda, MK Cromer, N Uchida, T Nishimura, R Romano, AJ Tong, VT Lemgart, J Camarena, M Pavel-Dinu, C Sindhu, V Wiebking, S Vaidyanath, DP Dever, RO Bak, A Laustsen, BJ Lesch, MR Jakobsen, V Sebastiano, H Nakauchi, MH Porteus
    Cell Stem Cell, 2019;24(5):821-828.e5.
  93. Species-specific developmental timing is maintained by pluripotent stem cells ex utero
    C Barry, MT Schmitz, P Jiang, MP Schwartz, BM Duffin, S Swanson, R Bacher, JM Bolin, AL Elwell, BE McIntosh, R Stewart, JA Thomson
    Dev. Biol., 2017;423(2):101-110.
  94. Intracellular pH Regulation in iPSCs-derived Astrocytes from Subjects with Chronic Mountain Sickness
    H Yao, H Zhao, J Wang, GG Haddad
    Neuroscience, 2018;0(0):.
  95. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases.
    Ishizaki et al.
    Mol.Pharmacol., 2000;57:976
  96. Chronic aerobic exercise improves insulin sensitivity and modulates Nrf2 and NF?kappaB/IkappaBalpha pathways in the skeletal muscle of rats fed with a high fat diet
    Q Yu, Z Xia, EC Liong, GL Tipoe
    Mol Med Rep, 2019;20(6):4963-4972.
  97. Urothelial organoids originating from Cd49fhigh mouse stem cells display Notch-dependent differentiation capacity.
    Santos et al.
    Nat.Commun., 2019;10:4407
  98. Direct conversion of normal and Alzheimer's Disease human fibroblasts into neuronal cells by small molecules.
    Hu et al.
    Cell Stem Cell, 2015;17:204
  99. Sufficiency for inducible Caspase-9 safety switch in human pluripotent stem cells and disease cells
    T Nishimura, H Xu, M Iwasaki, D Karigane, B Saavedra, Y Takahashi, FP Suchy, S Monobe, RM Martin, M Ohtaka, M Nakanishi, SR Burrows, ML Cleary, R Majeti, A Shibuya, H Nakauchi
    Gene Ther., 2020;0(0):.
  100. Production and validation of human induced pluripotent stem cell line from sporadic amyotrophic lateral sclerosis (SALS)
    J Ma, B Feng, D Kong, J He, R Guo, AE Amponsah, W Zhang, S Zhang, F Lv, Y Song, A Liu, H Cui
    Stem Cell Res, 2020;44(0):101760.
  101. Generation of two iPSC lines (FAMRCi004-A and FAMRCi004-B) from patient with familial progressive cardiac conduction disorder carrying genetic variant DSP p.His1684Arg
    A Khudiakov, K Perepelina, P Klauzen, A Zlotina, K Gusev, E Kaznacheye, A Malashiche, A Kostareva
    Stem Cell Res, 2020;43(0):101720.
  102. Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development
    EI Crosse, S Gordon-Key, S Rybtsov, A Binagui-Ca, H Felchle, NC Nnadi, K Kirschner, T Chandra, S Tamagno, DJ Webb, F Rossi, RA Anderson, A Medvinsky
    Cell Stem Cell, 2020;0(0):.
  103. In Vitro CRISPR/Cas9-Directed Gene Editing to Model LRRK2 G2019S Parkinson's Disease in Common Marmosets
    SC Vermilyea, A Babinski, N Tran, S To, S Guthrie, JH Kluss, JK Schmidt, GJ Wiepz, MG Meyer, ME Murphy, MR Cookson, ME Emborg, TG Golos
    Sci Rep, 2020;10(1):3447.
  104. The in vitro multilineage differentiation and maturation of lung and airway cells from human pluripotent stem cell-derived lung progenitors in 3D
    AL Rodrigues, HY Liu, YW Chen, M Porotto, A Moscona, HW Snoeck
    Nature Protocols, 2021;0(0):.
  105. Cytosine and adenosine base editing in human pluripotent stem cells using transient reporters for editing enrichment
    SJ Tekel, N Brookhouse, K Standage-B, X Wang, DA Brafman
    Nature Protocols, 2021;16(7):3596-3624.
  106. Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
    AS Genshaft, CGK Ziegler, CN Tzouanas, BE Mead, AM Jaeger, AW Navia, RP King, MD Mana, S Huang, V Mitsialis, SB Snapper, ÖH Yilmaz, T Jacks, JF Van Humbec, AK Shalek
    Nature Communications, 2021;12(1):4995.
  107. ATG7-mediated autophagy facilitates embryonic stem cell exit from naive pluripotency and marks commitment to differentiation
    J Zhou, H He, JJ Zhang, X Liu, W Yao, C Li, T Xu, SY Yin, DY Wu, CL Dou, Q Li, J Xiang, WJ Xiong, LY Wang, JM Tang, Z Xue, X Zhang, YL Miao
    Autophagy, 2022;0(0):.
  108. Malfunction of airway basal stem cells plays a crucial role in pathophysiology of tracheobronchopathia osteoplastica
    Y Hong, S Shan, Y Gu, H Huang, Q Zhang, Y Han, Y Dong, Z Liu, M Huang, T Ren
    Nature Communications, 2022;13(1):1309.
  109. Discovery and functional interrogation of SARS-CoV-2 protein-RNA interactions
    G Yeo, J Xiang, J Mueller, EC Luo, B Yee, D Schafer, J Schmok, F Tan, K Rothamel, R McVicar, E Kwong, B Croker, K Jones, HL Her, CY Chen, A Vu, W Jin, S Park, P Le, K Brennan, E Kofman, Y Li, A Tankka, K Dong, Y Song, A Clark, A Carlin, EV Nostrand, S Leibel
    Research square, 2022;0(0):.
  110. Site-specific O-GlcNAcylation of Psme3 maintains mouse stem cell pluripotency by impairing P-body homeostasis
    F Pecori, N Kondo, C Ogura, T Miura, M Kume, Y Minamijima, K Yamamoto, S Nishihara
    Cell Reports, 2021;36(2):109361.
  111. A pan-cancer organoid platform for precision medicine
    BM Larsen, M Kannan, LF Langer, BD Leibowitz, A Bentaieb, A Cancino, I Dolgalev, BE Drummond, JR Dry, CS Ho, G Khullar, BA Krantz, B Mapes, KE McKinnon, J Metti, JF Perera, TA Rand, V Sanchez-Fr, JM Shaxted, MM Stein, MA Streit, YC Tan, Y Zhang, E Zhao, J Venkataram, MC Stumpe, JA Borgia, A Masood, DVT Catenacci, JV Mathews, DB Gursel, JJ Wei, TH Welling, DM Simeone, KP White, AA Khan, C Igartua, AA Salahudeen
    Cell Reports, 2021;36(4):109429.
  112. Biphasic Activation of WNT Signaling Facilitates the Derivation of Midbrain Dopamine Neurons from hESCs for Translational Use
    TW Kim, J Piao, SY Koo, S Kriks, SY Chung, D Betel, ND Socci, SJ Choi, S Zabierowsk, BN Dubose, EJ Hill, EV Mosharov, S Irion, MJ Tomishima, V Tabar, L Studer
    Cell Stem Cell, 2021;28(2):343-355.e5.
  113. Patient Derived Colonoids as Drug Testing Platforms-Critical Importance of Oxygen Concentration
    HK Skovdahl, S Gopalakris, TD Svendsen, AVB Granlund, I Bakke, ZG Ginbot, S Thorsvik, A Flatberg, B Sporsheim, J Ostrop, TE Mollnes, AK Sandvik, T Bruland
    Frontiers in Pharmacology, 2021;12(0):679741.
  114. Fluvastatin promotes chondrogenic differentiation of adipose-derived mesenchymal stem cells by inducing bone morphogenetic protein 2
    M Kuwahara, Y Akasaki, N Goto, I Kurakazu, T Sueishi, M Toya, T Uchida, T Tsutsui, R Hirose, H Tsushima, Y Nakashima
    Bmc Pharmacology & Toxicology, 2022;23(1):61.
  115. De novo construction of T cell compartment in humanized mice engrafted with iPSC-derived thymus organoids
    A Zeleniak, C Wiegand, W Liu, C McCormick, R K, A Alavi, H Guan, S Bertera, R Lakomy, A Tajima, H Cohen, S Wong, L Balikani, B Mizerak, Z Bar-Joseph, M Trucco, I Banerjee, Y Fan
    Nature methods, 2022;0(0):.
  116. Stem-Cell-Derived beta-Like Cells with a Functional PTPN2 Knockout Display Increased Immunogenicity
    TM Triolo, JQ Matuschek, R Castro-Gut, AH Shilleh, SPM Williams, MS Hansen, K McDaniel, JM Barra, A Michels, HA Russ
    Cells, 2022;11(23):.
  117. Culture impact on the transcriptomic programs of primary and iPSC-derived human alveolar type 2 cells
    KD Alysandrat, C Garcia-de-, C Yao, P Pessina, J Huang, C Villacorta, OT Hix, K Minakin, CL Burgess, P Bawa, A Murthy, B Konda, MF Beers, BR Stripp, CF Kim, DN Kotton
    JCI Insight, 2022;0(0):.

Product Datasheets

COA view current batch
Or select another batch:
View Batch
SDS

Citations for Y-27632 dihydrochloride

The citations listed below are publications that use Tocris products. Selected citations for Y-27632 dihydrochloride include:

252 Citations: Showing 1 - 10

  1. In vitro reconstitution of epigenetic reprogramming in the human germ line.
    Authors: Murase Et al.
    Nature  2024;
  2. An in vivo neuroimmune organoid model to study human microglia phenotypes.
    Authors: Schafer Et al.
    Cell  2023;186:2111
  3. An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells
    Authors: Surendran Et al.
    STAR Protoc  2022;3:101803
  4. Pancreatic islet cryopreservation by vitrification achieves high viability, function, recovery and clinical scalability for transplantation.
    Authors: Zhan Et al.
    Nat.Med.  2022;28:798
  5. Biphasic activation of WNT signaling facilitates the derivation of midbrain dopamine neurons from hESCs for translational use.
    Authors: Kim Et al.
    Cell Stem Cell  2021;28:343
  6. Preclinical Efficacy and Safety of a Human Embryonic Stem Cell-Derived Midbrain Dopamine Progenitor Product, MSK-DA01
    Authors: Piao Et al.
    Cell Stem Cell  2021;28:217
  7. Generation of Complete Multi-Cell Type Lung Organoids From Human Embryonic and Patient-Specific Induced Pluripotent Stem Cells for Infectious Disease Modeling and Therapeutics Validation
    Authors: Leibel Et al.
    Curr Protoc Stem Cell Biol  2020;54:e118
  8. Reprogramming normal cells into tumour precursors requires ECM stiffness and oncogene-mediated changes of cell mechanical properties.
    Authors: Panciera Et al.
    Nat Mater  2020;19:797
  9. Efficient Generation of Trunk Neural Crest and Sympathetic Neurons from Human Pluripotent Stem Cells Via a Neuromesodermal Axial Progenitor Intermediate.
    Authors: Frith and Tsakiridis
    Curr Protoc Stem Cell Biol  2019;:e81
  10. Multiparametric rapid screening of neuronal process pathology for drug target identification in HSP patient-specific neurons.
    Authors: Rehbach Et al.
    Sci Rep  2019;9:9615
  11. User-defined morphogen patterning for directing human cell fate stratification.
    Authors: Regier Et al.
    Sci Rep  2019;9:6433
  12. Butyrate Produced by Commensal Bacteria Down-Regulates Indolamine 2,3-Dioxygenase 1 (IDO-1) Expression via a Dual Mechanism in Human Intestinal Epithelial Cells.
    Authors: Martin-Gallausiaux Et al.
    Front Immunol  2019;9:2838
  13. Esomeprazole Increases Airway Surface Liquid pH in Primary Cystic Fibrosis Epithelial Cells.
    Authors: Delpiano Et al.
    Front Pharmacol  2019;9:1462
  14. Quantification of human neuromuscular function through optogenetics.
    Authors: Vila Et al.
    Theranostics  2019;9:1232
  15. Automated real-time monitoring of human pluripotent stem cell aggregation in stirred tank reactors.
    Authors: Schwedhelm Et al.
    Sci Rep  2019;9:12297
  16. The adhesion-GPCR BAI1 shapes dendritic arbors via Bcr-mediated RhoA activation causing late growth arrest.
    Authors: Duman Et al.
    Elife  2019;8:e47566
  17. Evaluating Shigella flexneri pathogenesis in the human enteroid model.
    Authors: Ranganathan Et al.
    Infect Immun  2019;87
  18. The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and MetF. cytotoxicity in breast cancer.
    Authors: Zhuang Et al.
    Cancer Metab  2019;7:1
  19. Efficient generation of human primordial germ cell-like cells from pluripotent stem cells in a methylcellulose-based 3D system at large scale.
    Authors: Wang Et al.
    PeerJ  2019;6:e6143
  20. Highly Efficient CRISPR-Cas9-Mediated Genome Editing in Human Pluripotent Stem Cells.
    Authors: Maguire Et al.
    Curr Protoc Stem Cell Biol  2019;48:e64
  21. Protective effects of oxymatrine against DSS-induced acute intestinal inflammation in mice via blocking the RhoA/ROCK signaling pathway.
    Authors: Wang Et al.
    Biosci Rep  2019;39
  22. Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate.
    Authors: Crowell Et al.
    Cell Rep  2019;28:1499
  23. Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development.
    Authors: Genga Et al.
    Cell Rep  2019;27:708
  24. Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies.
    Authors: Mair Et al.
    Cell Rep  2019;27:599
  25. An Autaptic Culture System for Standardized Analyses of iPSC-Derived Human Neurons.
    Authors: Rhee Et al.
    Cell Rep  2019;27:2212
  26. A Single-Cell Model for Synaptic Transmission and Plasticity in Human iPSC-Derived Neurons.
    Authors: Meijer Et al.
    Cell Rep  2019;27:2199
  27. Entosis Controls a Developmental Cell Clearance in C. elegans.
    Authors: Lee Et al.
    Cell Rep  2019;26:3212
  28. Important Role of the GLP-1 Axis for Glucose Homeostasis after Bariatric Surgery.
    Authors: Larraufie Et al.
    Cell Rep  2019;26:1399
  29. A Stem-Cell-Derived Platform Enables Complete Cryptosporidium Development In Vitro and Genetic Tractability.
    Authors: Wilke Et al.
    Cell Host Microbe  2019;26:123
  30. Single-Cell Sequencing of iPSC-Da Neurons Reconstructs Disease Progression and Identifies HDAC4 as a Regulator of Parkinson Cell Phenotypes.
    Authors: Lang Et al.
    Cell Stem Cell  2019;24:93
  31. Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells.
    Authors: Montel-Hagen Et al.
    Cell Stem Cell  2019;24:376
  32. Establishment of porcine and human expanded potential stem cells.
    Authors: Gao Et al.
    Nat Cell Biol  2019;21:687
  33. Nuclear envelope deformation controls cell cycle progression in response to mechanical force.
    Authors: Aureille Et al.
    EMBO Rep  2019;20:e48084
  34. Constructing an Isogenic 3D Human Nephrogenic Progenitor Cell Model Composed of Endothelial, Mesenchymal, and SIX2-Positive Renal Progenitor Cells.
    Authors: Nguyen Et al.
    Stem Cells Int  2019;2019:3298432
  35. A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells.
    Authors: Chen Et al.
    Nat.Methods  2019;18:528
  36. Regional Activation of Myosin II in Cancer Cells Drives Tumor Progression via a Secretory Cross-Talk with the Immune Microenvironment.
    Authors: Georgouli Et al.
    Cell  2019;176:757
  37. Identification of Kinases Responsible for p53-Dependent Autophagy.
    Authors: Celano Et al.
    iScience  2019;15:109
  38. FGF Modulates the Axial Identity of Trunk hPSC-Derived Neural Crest but Not the Cranial-Trunk Decision.
    Authors: Hackland Et al.
    Stem Cell Reports  2019;12:920
  39. Chemical conversion of human fetal astrocytes into neurons through modulation of multiple signaling pathways.
    Authors: Yin Et al.
    Stem Cell Reports  2019;12:488
  40. A Non-canonical Pathway with Potential for Safer Modulation of Transforming Growth Factor-β1 in Steroid-Resistant Airway Diseases.
    Authors: Li Et al.
    iScience  2019;12:232
  41. Exosomes regulate neurogenesis and circuit assembly.
    Authors: Sharma Et al.
    Proc Natl Acad Sci U S A  2019;116:16086
  42. Repurposed quinacrine synergizes with CIS. reducing the effective dose required for treatment of head and neck squamous cell carcinoma.
    Authors: Bryant Et al.
    Oncotarget  2019;10:5229
  43. Targeted gene silencing in human embryonic stem cells using cell-penetrating peptide PepFect 14.
    Authors: Ervin Et al.
    Stem Cell Res Ther  2019;10:43
  44. Dual stem cell therapy synergistically improves cardiac function and vascular regeneration following myocardial infarction.
    Authors: Park Et al.
    Nat Commun  2019;10:3123
  45. Modulation of Wnt/BMP pathways during corneal differentiation of hPSC maintains ABCG2-positive LSC population that demonstrates increased regenerative potential.
    Authors: Vattulainen Et al.
    Stem Cell Res Ther  2019;10:236
  46. Long-term culture of mesenchymal stem cells impairs ATM-dependent recognition of DNA breaks and increases genetic instability.
    Authors: Hladik Et al.
    Stem Cell Res Ther  2019;10:218
  47. Dynamics of genome reorganization during human cardiogenesis reveal an RBM20-dependent splicing factory.
    Authors: Bertero Et al.
    Nat Commun  2019;10:1538
  48. TGF-β activation impairs fibroblast ability to support adult lung epithelial progenitor cell organoid formation.
    Authors: Ng-Blichfeldt Et al.
    Am J Physiol Lung Cell Mol Physiol  2019;
  49. Rho-Kinase Inhibition Ameliorates D.nib-Induced Endothelial Dysfunction and Pulmonary Hypertension.
    Authors: Fazakas Et al.
    Front Physiol  2018;9:537
  50. Bioreactor-based mass production of human iPSC-derived macrophages enables immunotherapies against bacterial airway infections.
    Authors: Ackermann Et al.
    Nat Commun  2018;9:5088
  51. A stably self-renewing adult blood-derived induced neural stem cell exhibiting patternability and epigenetic rejuvenation.
    Authors: Sheng Et al.
    Nat Commun  2018;9:4047
  52. Targeting the spliceosome for cutaneous squamous cell carcinoma therapy: a role for c-MYC and wild-type p53 in determining the degree of tumour selectivity.
    Authors: Hepburn Et al.
    Oncotarget  2018;9:23029
  53. Preclinical comparison of proteasome and ubiquitin E1 enzyme inhibitors in cutaneous squamous cell carcinoma: the identification of mechanisms of differential sensitivity.
    Authors: McHugh Et al.
    Oncotarget  2018;9:20265
  54. Effects of lung and airway epithelial maturation cocktail on the structure of lung bud organoids.
    Authors: Magro-Lopez Et al.
    Stem Cell Res.Ther.  2018;9:186
  55. Generating Loss-of-function iPSC Lines with Combined CRISPR Indel Formation and Reprogramming from Human Fibroblasts.
    Authors: Tidball Et al.
    Bio Protoc  2018;8
  56. Expansion of Airway Basal Cells and Generation of Polarized Epithelium.
    Authors: Levardon Et al.
    Bio Protoc  2018;8
  57. Rho-Kinase Inhibition Reduces Myofibroblast Differentiation and Proliferation of Scleral Fibroblasts Induced by Transforming Growth Factor β and Experimental Glaucoma.
    Authors: Pitha Et al.
    Transl Vis Sci Technol  2018;7:6
  58. Rho Kinase Inhibition by AT13148 Blocks Pancreatic Ductal Adenocarcinoma Invasion and Tumor Growth.
    Authors: Rath Et al.
    Cancer Res  2018;78:3321
  59. Lysophosphatidic Acid Induces ECM Production via Activation of the Mechanosensitive YAP/TAZ Transcriptional Pathway in Trabecular Meshwork Cells.
    Authors: Ho Et al.
    Invest Ophthalmol Vis Sci  2018;59:1969
  60. Advanced maturation of human cardiac tissue grown from pluripotent stem cells.
    Authors: Ronaldson-Bouchard Et al.
    Nature  2018;556:239
  61. α-Tubulin Acetyltransferase Is a Novel Target Mediating Neurite Growth Inhibitory Effects of Chondroitin Sulfate Proteoglycans and Myelin-Associated Glycoprotein.
    Authors: Wong Et al.
    Eneuro  2018;5
  62. Impaired adhesion of induced pluripotent stem cell-derived cardiac progenitor cells (iPSC-CPCs) to isolated extracellular matrix from failing hearts.
    Authors: McKown Et al.
    Heliyon  2018;4:e00870
  63. A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice.
    Authors: Ford Et al.
    Dev Cell  2018;47:509
  64. Derivation of Epithelial-Only Airway Organoids from Human Pluripotent Stem Cells.
    Authors: McCauley Et al.
    Curr Protoc Stem Cell Biol  2018;45:e51
  65. CRISPR/Cas9-based Targeted Genome Editing for the Development of Monogenic Diseases Models with Human Pluripotent Stem Cells.
    Authors: Gupta Et al.
    Curr Protoc Stem Cell Biol  2018;45:e50
  66. Retinoic acid signaling balances adult distal lung epithelial progenitor cell growth and differentiation.
    Authors: Ng-Blichfeldt Et al.
    EBioMedicine  2018;36:461
  67. PKCε Inhibits Neuronal Dendritic Spine Development through Dual Phosphorylation of Ephexin5.
    Authors: Schaffer Et al.
    Cell Rep  2018;25:2470
  68. Human Huntington's Disease iPSC-Derived Cortical Neurons Display Altered Transcriptomics, Morphology, and Maturation.
    Authors: Mehta Et al.
    Cell Rep  2018;25:1081
  69. Th17 lymphocytes induce neuronal cell death in a human iPSC-based model of Parkinson's disease.
    Authors: Sommer Et al.
    Cell Stem Cell.  2018;23:123
  70. Submucosal Gland Myoepithelial Cells Are Reserve Stem Cells That Can Regenerate Mouse Tracheal Epithelium.
    Authors: Lynch Et al.
    Cell Stem Cell  2018;22:653
  71. Mouse genome-wide association studies and systems genetics uncover the genetic architecture associated with hepatic pharmacokinetic and pharmacodynamic properties of a constrained ethyl antisense oligonucleotide targeting Malat1.
    Authors: Pirie Et al.
    PLoS Genet  2018;14:e1007732
  72. Neural stem cells for disease modeling and evaluation of therapeutics for infantile (CLN1/PPT1) and late infantile (CLN2/TPP1) neuronal ceroid lipofuscinoses.
    Authors: Sima Et al.
    Orphanet J Rare Dis  2018;13:54
  73. Use of human induced pluripotent stem cell-derived cardiomyocytes to assess drug cardiotoxicity.
    Authors: Sharma Et al.
    Nat Protoc  2018;13:3018
  74. Neural stem cells for disease modeling and evaluation of therapeutics for Tay-Sachs disease.
    Authors: Vu Et al.
    Orphanet J Rare Dis  2018;13:152
  75. Human Induced Pluripotent Stem Cell Production and Expansion from Blood using a Non-Integrating Viral Reprogramming Vector.
    Authors: Sharma Et al.
    Curr Protoc Mol Biol  2018;122:e58
  76. Generation of functioning nephrons by implanting human pluripotent stem cell-derived kidney progenitors.
    Authors: Bantounas Et al.
    Stem Cell Reports  2018;10:766
  77. Signaling-Dependent Control of Apical Membrane Size and Self-Renewal in Rosette-Stage Human Neuroepithelial Stem Cells.
    Authors: Medelnik Et al.
    Stem Cell Reports  2018;10:1751
  78. Differentiation of hepatocyte-like cells from human pluripotent stem cells using small molecules.
    Authors: Asumda Et al.
    Differentiation  2018;101:16
  79. ROCK signaling promotes collagen remodeling to facilitate invasive pancreatic ductal adenocarcinoma tumor cell growth.
    Authors: Rath Et al.
    EMBO Mol Med  2017;9:198
  80. Human Astrocyte Maturation Captured in 3D Cerebral Cortical Spheroids Derived from Pluripotent Stem Cells.
    Authors: Sloan Et al.
    Neuron  2017;95:779
  81. iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases.
    Authors: Abud Et al.
    Neuron  2017;94:278
  82. Concerted regulation of retinal pigment epithelium basement membrane and barrier function by angiocrine factors.
    Authors: Benedicto
    Nat Commun  2017;8:15374
  83. Dynamic behaviour of human neuroepithelial cells in the developing forebrain.
    Authors: Subramanian Et al.
    Nat Commun  2017;8:14167
  84. Membrane-bound ICAM-1 contributes to the onset of proinvasive tumor stroma by controlling acto-myosin contractility in carcinoma-associated fibroblasts.
    Authors: Bonan Et al.
    Oncotarget  2017;8:1304
  85. Atypical PKC and Notch Inhibition Differentially Modulate Cortical Interneuron Subclass Fate from Embryonic Stem Cells.
    Authors: Tischfield Et al.
    Stem Cell Reports  2017;8:1135
  86. Chemerin-induced arterial contraction is Gi- and calcium-dependent.
    Authors: Ferland Et al.
    Vascul Pharmacol  2017;88:30
  87. A pluripotent stem cell-based model for post-implantation human amniotic sac development.
    Authors: Shao
    Nat Commun  2017;8(1):208
  88. ASXL3 Is a Novel Pluripotency Factor in Human Respiratory Epithelial Cells and a Potential Therapeutic Target in Small Cell Lung Cancer.
    Authors: Shukla Et al.
    Cancer Res  2017;77:6267
  89. Discovery of Aromatic Carbamates that Confer Neuroprotective Activity by Enhancing Autophagy and Inducing the Anti-Apoptotic Protein B-Cell Lymphoma 2 (Bcl-2).
    Authors: Kinarivala Et al.
    J Med Chem  2017;60:9739
  90. Derivation of telencephalic oligodendrocyte progenitors from human pluripotent stem cells.
    Authors: Major Et al.
    Curr Protoc Stem Cell Biol  2017;39:1H.10.1
  91. A modular platform for differentiation of human PSCs into all major ectodermal lineages.
    Authors: Tchieu Et al.
    Cell Stem Cell.  2017;31:399
  92. Single-cell gene expression analysis reveals regulators of distinct cell subpopulations among developing human neurons.
    Authors: Wang Et al.
    Genome Res  2017;27:1783
  93. Induced Pluripotent Stem Cell-Derived Dopaminergic Neurons from Adult Common Marmoset Fibroblasts.
    Authors: Vermilyea Et al.
    Stem Cells Dev  2017;26:1225
  94. Differentiation of human pluripotent stem cells into functional lung alveolar epithelial cells.
    Authors: Kotton Et al.
    Cell Stem Cell.  2017;21:472
  95. Human Pluripotent Stem Cell-Derived Atrial and Ventricular Cardiomyocytes Develop from Distinct Mesoderm Populations.
    Authors: Lee Et al.
    Cell Stem Cell  2017;21:179
  96. Efficient Derivation of Functional Human Airway Epithelium from Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling.
    Authors: McCauley Et al.
    Cell Stem Cell  2017;20:844
  97. Human iPSC-Derived Cerebral Organoids Model Cellular Features of Lissencephaly and Reveal Prolonged Mitosis of Outer Radial Glia.
    Authors: Bershteyn Et al.
    Cell Stem Cell  2017;20:435
  98. Entosis Is Induced by Glucose Starvation.
    Authors: Hamann Et al.
    Cell Rep  2017;20:201
  99. In Vivo Human Somitogenesis Guides Somite Development from hPSCs.
    Authors: Xi
    Cell Rep  2017;18(6):1573
  100. Excitable Dynamics and Yap-Dependent Mechanical Cues Drive the Segmentation Clock.
    Authors: Hubaud Et al.
    Cell  2017;171:668
  101. Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency.
    Authors: Yang Et al.
    Cell  2017;169:243
  102. An essential role of CBL and CBL-B ubiquitin ligases in mammary stem cell maintenance.
    Authors: Mohapatra Et al.
    Development  2017;144:1072
  103. Mesoderm-specific Stat3 deletion affects expression of Sox9 yielding Sox9-dependent phenotypes.
    Authors: Hall
    PLoS Genet  2017;13(2):e1006610
  104. Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells.
    Authors: Morizane and Bonventre
    Nat Protoc  2017;12:195
  105. Directed differentiation and long-term maintenance of epicardial cells derived from human pluripotent stem cells under fully defined conditions.
    Authors: Bao Et al.
    Nat Protoc  2017;12:1890
  106. Neural stem cells for disease modeling of Wolman disease and evaluation of therapeutics.
    Authors: Aguisanda Et al.
    Orphanet J Rare Dis  2017;12:120
  107. The novel cannabinoid receptor GPR55 mediates anxiolytic-like effects in the medial orbital cortex of mice with acute stress.
    Authors: Shi
    Mol Brain  2017;10(1):38
  108. The receptor tyrosine kinase EphA2 promotes glutamine metabolism in tumors by activating the transcriptional coactivators YAP and TAZ.
    Authors: Edwards Et al.
    Sci Signal  2017;10
  109. An in vitro model of murine middle ear epithelium.
    Authors: Mulay Et al.
    Dis Model Mech  2016;9:1405
  110. Wnt and Neuregulin1/ErbB signalling extends 3D culture of hormone responsive mammary organoids
    Authors: Jardé Et al.
    Nature Communications  2016;7:13207
  111. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6.
    Authors: Guye Et al.
    Sci Rep  2016;7:10243
  112. Nogo-A controls structural plasticity at dendritic spines by rapidly modulating actin dynamics.
    Authors: Kellner Et al.
    Hippocampus  2016;6:816
  113. Risk of prenatal depression and stress treatment: alteration on serotonin system of offspring through exposure to Fluox..
    Authors: Pei Et al.
    Sci Rep  2016;6:33822
  114. DArgic neurons differentiating from LRRK2 G2019S induced pluripotent stem cells show early neuritic branching defects.
    Authors: Borgs Et al.
    Scientific Reports  2016;6:33377
  115. Regulation of actin dynamics by WNT-5A: implications for human airway smooth muscle contraction.
    Authors: Koopmans Et al.
    Sci Rep  2016;6:30676
  116. Tumor-induced remote ECM network orientation steers angiogenesis.
    Authors: Balcioglu Et al.
    Development  2016;6:22580
  117. Induced Pluripotent Stem Cells for Disease Modeling and Evaluation of Therapeutics for Niemann-Pick Disease Type A.
    Authors: Long Et al.
    Stem Cells Transl Med  2016;5:1644
  118. Local mechanical forces promote polarized junctional assembly and axis elongation in Drosophila.
    Authors: Yu and Fernandez-Gonzalez
    Elife  2016;5
  119. ROCK signalling induced gene expression changes in mouse pancreatic ductal adenocarcinoma cells.
    Authors: Rath Et al.
    Sci Data  2016;3:160101
  120. Elevated Glucose Levels Promote Contractile and Cytoskeletal Gene Expression in Vascular Smooth Muscle via Rho/Protein Kinase C and Actin Polymerization.
    Authors: Hien Et al.
    J Biol Chem  2016;291:3552
  121. Capturing the biology of disease severity in a PSC-based model of familial dysautonomia.
    Authors: Zeltner Et al.
    Nat Med  2016;22:1421
  122. Cryopreservation of Brain Endothelial Cells Derived from Human Induced Pluripotent Stem Cells Is Enhanced by Rho-Associated Coiled Coil-Containing Kinase Inhibition.
    Authors: Wilson Et al.
    Tissue Eng Part C Methods  2016;22:1085
  123. Dual SMAD Signaling Inhibition Enables Long-Term Expansion of Diverse Epithelial Basal Cells
    Authors: Mou Et al.
    Cell: Stem Cell  2016;19:217
  124. Molecular Diversity of Midbrain Development in Mouse, Human, and Stem Cells.
    Authors: Manno Et al.
    Cell  2016;167:566
  125. Human Enteroids as a Model of Upper Small Intestinal Ion Transport Physiology and Pathophysiology.
    Authors: Foulke-Abel Et al.
    Nat Commun  2016;150:638
  126. Generating high-purity cardiac and endothelial derivatives from patterned mesoderm using humanpluripotent stem cells.
    Authors: Palpant Et al.
    Nature Protocols  2016;12:15
  127. Genomic Instability Associated with p53 Knockdown in the Generation of Huntington's Disease Human Induced Pluripotent Stem Cells.
    Authors: Tidball Et al.
    PLoS One  2016;11:e0150372
  128. Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro.
    Authors: Chal Et al.
    Nat.Protoc.  2016;11:1833
  129. Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells.
    Authors: Stebbins Et al.
    Methods  2016;101:93
  130. Comparison of Human Embryonic Stem Cell-Derived Cardiomyocytes, Cardiovascular Progenitors, and Bone Marrow Mononuclear Cells for Cardiac Repair.
    Authors: Fernandes Et al.
    Bone Res  2015;NULL
  131. A transcriptome-based classifier to identify developmental toxicants by stem cell testing: design, validation and optimization for histone deacetylase inhibitors.
    Authors: Rempel Et al.
    Arch Toxicol  2015;89:1599
  132. Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue.
    Authors: Sakaguchi Et al.
    Mol Vis  2015;6:8896
  133. A Rac/Cdc42 exchange factor complex promotes formation of lateral filopodia and blood vessel lumen morphogenesis.
    Authors: Abraham Et al.
    Stem Cell Reports  2015;6:7286
  134. Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.
    Authors: Edri Et al.
    Nat Commun  2015;6:6500
  135. Substrate stress relaxation regulates cell spreading.
    Authors: Chaudhuri Et al.
    Brain  2015;6:6364
  136. A therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement.
    Authors: Luzzani Et al.
    Int J Cancer  2015;6:6
  137. Cloning-free CRISPR.
    Authors: Arbab Et al.
    Cell  2015;5:908
  138. Machine learning plus optical flow: a simple and sensitive method to detect cardioactive drugs.
    Authors: Lee Et al.
    Sci Rep  2015;5:11817
  139. Dynamic tensile forces drive collective cell migration through three-dimensional extracellular matrices.
    Authors: Gjorevski Et al.
    Sci Rep  2015;5:11458
  140. A panel of induced pluripotent stem cells from chimpanzees: a resource for comparative functional genomics.
    Authors: Romero Et al.
    Elife  2015;4:e07103
  141. Defined conditions for the isolation and expansion of basal prostate progenitor cells of mouse and human origin.
    Authors: Höfner Et al.
    PLoS One  2015;4:503
  142. Large-scale hematopoietic differentiation of human induced pluripotent stem cells provides granulocytes or macrophages for cell replacement therapies.
    Authors: Lachmann Et al.
    Nat Commun  2015;4:282
  143. Rho/Rock signal transduction pathway is required for MSC tenogenic differentiation.
    Authors: Maharam Et al.
    Stem Cell Res Ther  2015;3:15015
  144. Nephron organoids derived from human pluripotent stem cells model kidney development and injury.
    Authors: Morizane Et al.
    Nat Commun  2015;33:1193
  145. Odontogenic Ameloblast-associated Protein (ODAM) Mediates Junctional Epithelium Attachment to Teeth via Integrin-ODAM-Rho Guanine Nucleotide Exchange Factor 5 (ARHGEF5)-RhoA Signaling.
    Authors: Lee Et al.
    Toxicol Sci  2015;290:14740
  146. Phenotypes of trypsin- and Col. -prepared bovine corneal endothelial cells in the presence of a selective Rho kinase inhibitor, Y-27632.
    Authors: Su Et al.
    PLoS Comput Biol  2015;21:633
  147. RhoA GTPase-induced ocular hypertension in a rodent model is associated with increased fibrogenic activity in the trabecular meshwork.
    Authors: Pattabiraman Et al.
    Autophagy  2015;185:496
  148. Transgenic plants as low-cost platform for chemotherapeutic drugs screening.
    Authors: Vergara Et al.
    J Biol Chem  2015;16:2174
  149. Human embryonic stem cell-derived oligodendrocyte progenitors remyelinate the brain and rescue behavioral deficits following radiation.
    Authors: Piao Et al.
    Cell Stem Cell  2015;16:198
  150. Hypoxia and loss of PHD2 inactivate stromal fibroblasts to decrease tumour stiffness and metastasis.
    Authors: Madsen Et al.
    EMBO Rep  2015;16:1394
  151. Cortical contractility triggers a stochastic switch to fast amoeboid cell motility.
    Authors: Ruprecht Et al.
    Int J Mol Sci  2015;160:673
  152. Comprehensive analysis of microRNA expression in regionalized human neural progenitor cells reveals microRNA-10 as a caudalizing factor.
    Authors: Jönsson Et al.
    Int J Nanomedicine  2015;142:3166
  153. Duration of culture and sonic hedgehog signaling differentially specify PV versus SST cortical interneuron fates from embryonic stem cells.
    Authors: Tyson Et al.
    Stem Cells  2015;142:1267
  154. Early maturation and distinct tau pathology in induced pluripotent stem cell-derived neurons from patients with MAPT mutations.
    Authors: Iovino Et al.
    Development  2015;138:3345
  155. SDF-1α stiffens myeloma bone marrow mesenchymal stromal cells through the activation of RhoA-ROCK-Myosin II.
    Authors: Choi Et al.
    Stem Cell Reports  2015;136:E219
  156. The integrin expression profile modulates orientation and dynamics of force transmission at cell-matrix adhesions.
    Authors: Balcioglu Et al.
    Nat Biotechnol  2015;128:1316
  157. Quantitative Live Imaging of Human Embryonic Stem Cell Derived Neural Rosettes Reveals Structure-Function Dynamics Coupled to Cortical Development.
    Authors: Ziv Et al.
    Stem Cell Reports  2015;11:e1004453
  158. Variations in Glycogen Synthesis in Human Pluripotent Stem Cells with Altered Pluripotent States.
    Authors: Chen Et al.
    PLoS One  2015;10:e0142554
  159. The Viral Mimetic Polyinosinic:Polycytidylic Acid Alters the Growth Characteristics of Small Intestinal and Colonic Crypt Cultures.
    Authors: Davies Et al.
    PLoS One  2015;10:e0138531
  160. SimV. Increases Fibulin-2 Expression in Human Coronary Artery Smooth Muscle Cells via RhoA/Rho-Kinase Signaling Pathway Inhibition.
    Authors: Serra Et al.
    PLoS One  2015;10:e0133875
  161. Optimization of Invasion-Specific Effects of Betulin Derivatives on Prostate Cancer Cells through Lead Development.
    Authors: Härmä Et al.
    Stem Cell Reports  2015;10:e0126111
  162. SimV. enhances Rho/actin/cell rigidity pathway contributing to mesenchymal stem cells' osteogenic differentiation.
    Authors: Tai Et al.
    Nat Commun  2015;10:5881
  163. Molecular beacon-enabled purification of living cells by targeting cell type-specific mRNAs.
    Authors: Wile Et al.
    Nat Protoc  2014;9:2411
  164. Development of a primary mouse intestinal epithelial cell monolayer culture system to evaluate factors that modulate IgA transcytosis.
    Authors: Moon Et al.
    Mucosal Immunol  2014;7:818
  165. TRPA1 is essential for the vascular response to environmental cold exposure.
    Authors: Aubdool Et al.
    Korean J Physiol Pharmacol  2014;5:5732
  166. Diverse matrix metalloproteinase functions regulate cancer amoeboid migration.
    Authors: Orgaz Et al.
    Nat Commun  2014;5:4255
  167. Sucrose non-fermenting related kinase enzyme is essential for cardiac metabolism.
    Authors: Cossette Et al.
    Biol Open  2014;4:48
  168. Prospective identification of functionally distinct stem cells and neurosphere-initiating cells in adult mouse forebrain.
    Authors: Mich Et al.
    Elife  2014;3:e02669
  169. Derivation and expansion of PAX7-positive muscle progenitors from human and mouse embryonic stem cells.
    Authors: Shelton Et al.
    Stem Cell Reports  2014;3:516
  170. The atypical Rho GTPase, RhoU, regulates cell-adhesion molecules during cardiac morphogenesis.
    Authors: Dickover Et al.
    Dev Biol  2014;389:182
  171. Age-dependent contribution of Rho kinase in CB-induced contraction of human detrusor smooth muscle in vitro.
    Authors: Kirschstein Et al.
    Acta Pharmacol Sin  2014;35:74
  172. Efficient generation of lung and airway epithelial cells from human pluripotent stem cells.
    Authors: Huang Et al.
    Nat Biotechnol  2014;32:84
  173. Cryopreservation of human pluripotent stem cells in defined medium.
    Authors: Liu and Chen
    Curr Protoc Stem Cell Biol  2014;31:1C.17.1
  174. Generation of GFP Reporter Human Induced Pluripotent Stem Cells Using AAVS1 Safe Harbor Transcription Activator-Like Effector Nuclease.
    Authors: Luo Et al.
    Curr Protoc Stem Cell Biol  2014;29:5A.7.1
  175. Role of Rho GDP dissociation inhibitor α in control of epithelial sodium channel (ENaC)-mediated sodium reabsorption.
    Authors: Pavlov Et al.
    J Biol Chem  2014;289:28651
  176. Hippo-independent activation of YAP by the GNAQ uveal melanoma oncogene through a trio-regulated rho GTPase signaling circuitry.
    Authors: Feng Et al.
    Cancer Cell  2014;25:831
  177. GABAergic terminals are a source of galanin to modulate cholinergic neuron development in the neonatal forebrain.
    Authors: Keimpema Et al.
    Cereb Cortex  2014;24:3277
  178. Competition between human cells by entosis.
    Authors: Sun Et al.
    Cell Res  2014;24:1299
  179. Induction of entosis by epithelial cadherin expression.
    Authors: Sun Et al.
    Cell Res  2014;24:1288
  180. Transforming growth factor β-induced superficial zone protein accumulation in the surface zone of articular cartilage is dependent on the cytoskeleton.
    Authors: McNary Et al.
    Tissue Eng Part A  2014;20:921
  181. An in vivo role for Rho kinase activation in the tumour vascular disrupting activity of combretastatin A-4 3-O-phosphate.
    Authors: Williams Et al.
    Br J Pharmacol  2014;171:4902
  182. A high-throughput screen for teratogens using human pluripotent stem cells.
    Authors: Kameoka Et al.
    J Biomol Screen  2014;137:76
  183. Signals from the surface modulate differentiation of human pluripotent stem cells through glycosaminoglycans and integrins.
    Authors: Wrighton Et al.
    Circ Res  2014;111:18126
  184. The role of mechanical force and ROS in integrin-dependent signals.
    Authors: Zeller Et al.
    PLoS One  2013;8:e64897
  185. Fluid-phase pinocytosis of native low density lipoprotein promotes murine M-CSF differentiated macrophage foam cell formation.
    Authors: Barthwal Et al.
    PLoS One  2013;8:e58054
  186. Induced pluripotent stem cells from patients with human fibrodysplasia ossificans progressiva show increased mineralization and cartilage formation.
    Authors: Matsumoto Et al.
    Orphanet J Rare Dis  2013;8:190
  187. Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions.
    Authors: Lian Et al.
    Nat Protoc  2013;8:162
  188. Generating regionalized neuronal cells from pluripotency, a step-by-step protocol.
    Authors: Kirkeby Et al.
    Front Cell Neurosci  2013;6:64
  189. Activation of β 1 but not β 3 integrin increases cell traction forces.
    Authors: Lin Et al.
    J Neurosci  2013;587:763
  190. Generation of integration-free and region-specific neural progenitors from primate fibroblasts.
    Authors: Lu Et al.
    Curr Biol  2013;3:1580
  191. PI3K regulates branch initiation and extension of cultured mammary epithelia via Akt and Rac1 respectively.
    Authors: Zhu and Nelson
    Cell  2013;379:235
  192. Calcium release-dependent actin flow in the leading process mediates axophilic migration.
    Authors: Hutchins Et al.
    J Cell Biochem  2013;33:11361
  193. mTOR regulates phagosome and entotic vacuole fission.
    Authors: Krajcovic Et al.
    Mol Biol Cell  2013;24:3736
  194. Sox10-MCS5 enhancer dynamically tracks human oligodendrocyte progenitor fate.
    Authors: Pol Et al.
    Stem Cell Res  2013;247:694
  195. A phenotypic screening assay for modulators of huntingtin-induced transcriptional dysregulation
    Authors: Lazzeroni Et al.
    J Biomol Screen  2013;18:984
  196. A phenotypic screening assay for modulators of huntingtin-induced transcriptional dysregulation.
    Authors: Lazzeroni Et al.
    Exp Neurol  2013;18:984
  197. Inhibition of Rho-kinase improves erectile function, increases nitric oxide signaling and decreases penile apoptosis in a rat model of cavernous nerve injury.
    Authors: Hannan Et al.
    Am J Physiol Lung Cell Mol Physiol  2013;189:1155
  198. Mechanotransduction and YAP-dependent matrix remodelling is required for the generation and maintenance of cancer-associated fibroblasts.
    Authors: Calvo Et al.
    Nat Cell Biol  2013;15:637
  199. The microtubule end-binding protein EB2 is a central regulator of microtubule reorganisation in apico-basal epithelial differentiation.
    Authors: Goldspink Et al.
    J Cell Sci  2013;126:4000
  200. Stepwise, non-adherent differentiation of human pluripotent stem cells to generate basal forebrain cholinergic neurons via hedgehog signaling.
    Authors: Crompton Et al.
    Nat Cell Biol  2013;11:1206
  201. Interleukin-6 and oncostatin-M synergize with the PI3K/AKT pathway to promote aggressive prostate malignancy in mouse and human tissues.
    Authors: Smith Et al.
    Mol Cancer Res  2013;11:1159
  202. Shaping organs by a wingless-int/Notch/nonmuscle myosin module which orients feather bud elongation.
    Authors: Li Et al.
    Proc Natl Acad Sci U S A  2013;110:E1452
  203. ZFX controls the self-renewal of human embryonic stem cells.
    Authors: Harel Et al.
    PLoS One  2012;7:e42302
  204. Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions.
    Authors: Beers Et al.
    Nat Protoc  2012;7:2029
  205. DMH1, a highly selective small molecule BMP inhibitor promotes neurogenesis of hiPSCs: comparison of PAX6 and SOX1 expression during neural induction.
    Authors: Neely Et al.
    Proc Natl Acad Sci U S A  2012;3:482
  206. Soluble epoxide hydrolase: sex differences and role in endothelial cell survival.
    Authors: Gupta Et al.
    Arterioscler Thromb Vasc Biol  2012;32:1936
  207. Intra-axonal translation of RhoA promotes axon growth inhibition by CSPG.
    Authors: Walker Et al.
    J Cell Sci  2012;32:14442
  208. Pregnancy reduces RhoA/Rho kinase and protein kinase C signaling pathways downstream of thromboxane receptor activation in the rat uterine artery.
    Authors: Goulopoulou Et al.
    Am J Physiol Heart Circ Physiol  2012;302:H2477
  209. Neuritogenesis: the prion protein controls β1 integrin signaling activity.
    Authors: Loubet Et al.
    FASEB J  2012;26:678
  210. A temporal chromatin signature in human embryonic stem cells identifies regulators of cardiac development.
    Authors: Paige Et al.
    Cell  2012;151:221
  211. Slug and Sox9 cooperatively determine the mammary stem cell state.
    Authors: Guo Et al.
    J Cell Sci  2012;148:1015
  212. Synthetic lethal screening with small-molecule inhibitors provides a pathway to rational combination therapies for melanoma.
    Authors: Roller Et al.
    Mol Cancer Ther  2012;11:2505
  213. Measurement and analysis of traction force dynamics in response to vasoactive agonists.
    Authors: Yang Et al.
    Integr Biol (Camb)  2011;3:663
  214. RhoJ/TCL regulates endothelial motility and tube formation and modulates actomyosin contractility and focal adhesion numbers.
    Authors: Kaur Et al.
    Arterioscler Thromb Vasc Biol  2011;31:657
  215. Cell wound repair in Drosophila occurs through three distinct phases of membrane and cytoskeletal remodeling.
    Authors: Abreu-Blanco Et al.
    J Cell Biol  2011;193:455
  216. The Relaxing Effect of α-Defensin 1 on the Adrenergic Responses of Rat Bladder.
    Authors: Lee Et al.
    Dev Biol  2011;15:143
  217. Wnt signaling specifies and patterns intestinal endoderm.
    Authors: Sherwood Et al.
    Mech Dev  2011;128:387
  218. An association between NUAK2 and MRIP reveals a novel mechanism for regulation of actin stress fibers.
    Authors: Vallenius Et al.
    Integr Biol (Camb)  2011;124:384
  219. Assessment of chemical-induced impairment of human neurite outgrowth by multiparametric live cell imaging in high-density cultures.
    Authors: Stiegler Et al.
    Toxicol Sci  2011;121:73
  220. STAT inhibit angiotensin II/Smad pathway and related vascular fibrosis, by a TGF-β-independent process.
    Authors: Díez Et al.
    PLoS One  2010;5:e14145
  221. Endogenous patterns of mechanical stress are required for branching morphogenesis.
    Authors: Gjorevski and Nelson
    Stroke  2010;2:424
  222. Cortical actin dynamics facilitate early-stage centrosome separation.
    Authors: Cao Et al.
    J Pharmacol Exp Ther  2010;20:770
  223. Rho-kinase directs Bazooka/Par-3 planar polarity during Drosophila axis elongation.
    Authors: Simões Et al.
    Dev Cell  2010;19:377
  224. Tissue geometry patterns epithelial-mesenchymal transition via intercellular mechanotransduction.
    Authors: Gomez Et al.
    ACS Chem Neurosci  2010;110:44
  225. PDGF signaling is required for epicardial function and blood vessel formation in regenerating zebrafish hearts.
    Authors: Kim Et al.
    FEBS Lett  2010;107:17206
  226. The Rho-A/Rho-kinase pathway is up-regulated but remains inhibited by cyclic guanosine monophosphate-dependent mechanisms during endotoxemia in small mesenteric arteries.
    Authors: Silva-Santos Et al.
    J Biol Chem  2009;37:1716
  227. Cell polarity triggered by cell-cell adhesion via E-cadherin.
    Authors: Desai Et al.
    Br J Pharmacol  2009;122:905
  228. Localized and reversible TGFβ signalling switches breast cancer cells from cohesive to single cell motility.
    Authors: Giampieri Et al.
    Nat Commun  2009;11:1287
  229. Essential role of TGF-beta/Smad pathway on statin dependent vascular smooth muscle cell regulation.
    Authors: Rodríguez-Vita Et al.
    PLoS One  2008;3:e3959
  230. Emergence of patterned stem cell differentiation within multicellular structures.
    Authors: Ruiz and Chen
    J Cell Sci  2008;26:2921
  231. Antiepileptic effects of two Rho-kinase inhibitors, Y-27632 and fasudil, in mice.
    Authors: Inan and Büyükafşar
    Am J Pathol  2008;155:44
  232. Quantifying myosin light chain phosphorylation in single adherent cells with automated fluorescence microscopy.
    Authors: Bhadriraju Et al.
    J Biol Chem  2007;8:43
  233. Ins induces airway smooth muscle contraction.
    Authors: Schaafsma Et al.
    Cell Rep  2007;150:136
  234. Fn14 is upregulated in cytokine-stimulated vascular smooth muscle cells and is expressed in human carotid atherosclerotic plaques: modulation by AtoVas.
    Authors: Muñoz-García Et al.
    Proc Natl Acad Sci U S A  2006;37:2044
  235. Cingulin regulates claudin-2 expression and cell proliferation through the small GTPase RhoA.
    Authors: Guillemot and Citi
    Mol Biol Cell  2006;17:3569
  236. ROCK- and myosin-dependent matrix deformation enables protease-independent tumor-cell invasion in vivo.
    Authors: Wyckoff Et al.
    Curr Biol  2006;16:1515
  237. Temporally and spatially coordinated roles for Rho, Rac, Cdc42 and their effectors in growth cone guidance by a physiological electric field.
    Authors: Rajnicek Et al.
    J Cell Sci  2006;119:1723
  238. Endothelin-1, via ETA receptor and independently of transforming growth factor-β, increases the connective tissue growth factor in vascular smooth muscle cells.
    Authors: Rodríguez-Vita Et al.
    Am J Physiol Cell Physiol  2005;97:125
  239. CXCR4 chemokine receptor and integrin signaling co-operate in mediating adhesion and chemoresistance in small cell lung cancer (SCLC) cells.
    Authors: Hartmann Et al.
    Oncogene  2005;24:4462
  240. Signal transduction underlying CB-induced contraction of human urinary bladder.
    Authors: Schneider Et al.
    J Biol Chem  2004;309:1148
  241. Signal transduction underlying CB-induced contraction of rat urinary bladder. II. Protein kinases.
    Authors: Fleichman Et al.
    J Pharmacol Exp Ther  2004;308:54
  242. Regulation of MDCK cell-substratum adhesion by RhoA and myosin light chain kinase after ATP depletion.
    Authors: Prahalad Et al.
    Am J Physiol Cell Physiol  2004;286:C693
  243. The activity of the plexin-A1 receptor is regulated by Rac.
    Authors: Turner Et al.
    J Biol Chem  2004;279:33199
  244. Myosin phosphatase-Rho interacting protein. A new member of the myosin phosphatase complex that directly binds RhoA.
    Authors: Surks Et al.
    J Biol Chem  2003;278:51484
  245. Effects of rho kinase and actin stress fibers on sustained extracellular signal-regulated kinase activity and activation of G(1) phase cyclin-dependent kinases.
    Authors: Roovers and Assoian
    Mol Cell Biol  2003;23:4283
  246. Expression and functional role of Rho-kinase in rat urinary bladder smooth muscle.
    Authors: Wibberley Et al.
    Br J Pharmacol  2003;138:757
  247. Comparison of signalling mechanisms involved in rat mesenteric microvessel contraction by noradrenaline and sphingosylphosphorylcholine.
    Authors: Altmann Et al.
    Br J Pharmacol  2003;138:261
  248. Rho protein inactivation induced apoptosis of cultured human endothelial cells.
    Authors: Hippenstiel Et al.
    BMC Cell Biol  2002;283:L830
  249. Stiffness changes in cultured airway smooth muscle cells.
    Authors: An Et al.
    Gastroenterology  2002;283:C792
  250. Interaction of cofilin with triose-phosphate isomerase contributes glycolytic fuel for Na,K-ATPase via Rho-mediated signaling pathway.
    Authors: Jung Et al.
    Br J Pharmacol  2002;277:48931
  251. Differential regulation of cell migration, actin stress fiber organization, and cell transformation by functional domains of Crk-associated substrate.
    Authors: Huang Et al.
    Crit Care Med  2002;277:27265
  252. Myelin-associated glycoprotein interacts with ganglioside GT1b. A mechanism for neurite outgrowth inhibition.
    Authors: Vinson Et al.
    J Urol  2001;276:20280

FAQs

No product specific FAQs exist for this product, however you may

View all Small Molecule FAQs

Reviews for Y-27632 dihydrochloride

Average Rating: 5 (Based on 4 Reviews)

5 Star
23.53%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Y-27632 dihydrochloride?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Great for increasing organoids viability
By MARIA PINNA on 05/17/2023
Species: Human

Supplementing the organoids culture media with Y-27632 dihydrochloride after passaging prevents cell death and increases viability.

In vitro study
By Emma Watson on 08/31/2021
Species: Human

for human iPSC cells

PDX organoid culture
By Puru Lamichhane on 03/14/2021
Species: Human

PDX organoid culture

Human PDAC orgnaoids
By Anonymous on 05/03/2019
Species: Human

To make Human PDAC orgnaoids

Human PDAC orgnaoids 1254
Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.