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Mouse CCL17/TARC Antibody

Catalog #: MAB529
18 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
MAB529-SP
MAB529-500
MAB529-100
Chemotaxis Induced by CCL17/TARC and Neutralization by Mouse CCL17/TARC Antibody.
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Product Details
Citations (18)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL17/TARC Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL17/TARC in ELISAs.
Source
Monoclonal Rat IgG2A Clone # 110904
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived Recombinant Mouse CCL17/TARC
Ala24-Pro93
Accession # Q9WUZ6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample

Mouse CCL17/TARC Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse CCL17/TARC Biotinylated Antibody (Catalog # BAF529)

Standard: Recombinant Mouse CCL17/TARC Protein (Catalog # 529-TR)

Neutralization
Measured by its ability to neutralize CCL17/TARC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 0.75-3.0 µg/mL in the presence of 20 ng/mL Recombinant Mouse CCL17/TARC.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL17/TARC and Neutralization by Mouse CCL17/TARC Antibody. View Larger

Chemotaxis Induced by CCL17/TARC and Neutralization by Mouse CCL17/TARC Antibody. Recombinant Mouse CCL17/TARC (Catalog # 529-TR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL17/TARC (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CCL17/TARC Monoclonal Antibody (Catalog # MAB529). The ND50 is typically 0.75-3.0 µg/mL.

Block/ Neutralize Detection of Mouse CCL17/TARC by Block/Neutralize View Larger

Detection of Mouse CCL17/TARC by Block/Neutralize CCL17-mediated chemotaxis is inhibited in a dose-dependent manner by B202 and B225.EL4 cells were incubated with mouse CCL17 at 2/mL in the presence and absence of (A) B202, (B) B225 (C) CNTO 5516 which is an irrelevant rat:mouse chimeric antibody having a mouse IgG1 Fc or (D) MAB529 (rat antibody) which is a commercial antibody that neutralizes CCL17. Assay was repeated twice with five replicates per sample each time. Data are expressed as chemotactic index (CI = Average RLU from stimulated wells/average RLU from medium control; medium control = CI of 1). Antibody dose used for each sample is indicated along the x axis. Composite CI results from both experiments are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24339934), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL17/TARC

Human thymus and activation-regulated chemokine (TARC) also known as CCL17, is a novel CC chemokine identified using a signal sequence trap method. Mouse CCL17 was discovered as a dendritic cell (DC) specific gene by differentiation RNA display. Mouse CCL17 cDNA encodes a highly basic 93 amino acid (aa) residue precursor protein with a 23 aa residue putative signal peptide that is cleaved to generate the 70 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3, and I-309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Mouse CCL17 is constitutively expressed in thymic DC, and at a lower level in lymph node DC in the lung. Recombinant CCL17 has been shown to be chemotactic for T cell lines and antigen-primed T helper cells. In humans, CCL17 was identified to be a specific functional ligand for CCR4 and CCR8, receptors that are selectively expressed on T cells.

References
  1. Imai, T. et al. (1997) J. Biol. Chem. 272:15036.
  2. Imai, T. et al. (1996) J. Biol. Chem. 271:21514.
  3. Nomiyama, H. et al. (1997) Genomics 40:211.
  4. Lieberam, I. et al. (1999) Eur. J. Immunol. 29:2684.
Entrez Gene IDs
6361 (Human); 20295 (Mouse)
Alternate Names
ABCD-2; CC chemokine TARC; C-C motif chemokine 17; CCL17; chemokine (C-C motif) ligand 17; MGC138273; SCYA17; SCYA17MGC138271; small inducible cytokine subfamily A (Cys-Cys), member 17; Small-inducible cytokine A17; T cell-directed CC chemokine; TARC; TARCA-152E5.3; Thymus and activation-regulated chemokine

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Citations for Mouse CCL17/TARC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. CCL17 acts as a novel therapeutic target in pathological cardiac hypertrophy and heart failure
    Authors: Yang Zhang, Yicong Ye, Xiaoqiang Tang, Hui Wang, Toshiko Tanaka, Ran Tian et al.
    Journal of Experimental Medicine
  2. Surrogate Antibodies That Specifically Bind and Neutralize CCL17 But Not CCL22
    Authors: Sandra Santulli-Marotto, Jamie Fisher, Theodore Petley, Ken Boakye, Tadas Panavas, Jennifer Luongo et al.
    Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
  3. Recombinant CCL17-dependent CCR4 activation alleviates neuroinflammation and neuronal apoptosis through the PI3K/AKT/Foxo1 signaling pathway after ICH in mice
    Authors: S Deng, P Jin, P Sherchan, S Liu, Y Cui, L Huang, JH Zhang, Y Gong, J Tang
    Journal of Neuroinflammation, 2021-03-01;18(1):62.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. CCL17 blockade as a therapy for osteoarthritis pain and disease
    Authors: MC Lee, R Saleh, A Achuthan, AJ Fleetwood, I Förster, JA Hamilton, AD Cook
    Arthritis Res. Ther., 2018-04-05;20(1):62.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  5. TNF and granulocyte macrophage-colony stimulating factor interdependence mediates inflammation via CCL17
    Authors: AD Cook, MC Lee, R Saleh, HW Khiew, AD Christense, A Achuthan, AJ Fleetwood, DC Lacey, JE Smith, I Förster, JA Hamilton
    JCI Insight, 2018-03-22;3(6):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  6. RNA Aptamers Recognizing Murine CCL17 Inhibit T Cell Chemotaxis and Reduce Contact Hypersensitivity In�Vivo
    Authors: L Fülle, N Steiner, M Funke, F Gondorf, F Pfeiffer, J Siegl, FV Opitz, SK Ha beta el, AB Erazo, O Schanz, HJ Stunden, M Blank, C Gröber, K Händler, M Beyer, H Weighardt, E Latz, JL Schultze, G Mayer, I Förster
    Mol. Ther., 2017-10-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  7. iNKT Cell Emigration out of the Lung Vasculature Requires Neutrophils and Monocyte-Derived Dendritic Cells in Inflammation
    Cell Rep, 2016-09-20;16(12):3260-72.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  8. Granulocyte macrophage colony-stimulating factor induces CCL17 production via IRF4 to mediate inflammation
    J Clin Invest, 2016-08-15;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  9. M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections.
    Authors: Ohama H, Asai A, Ito I, Suzuki S, Kobayashi M, Higuchi K, Suzuki F
    Am J Pathol, 2014-12-06;185(2):420-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Selection
  10. Engagement of two distinct binding domains on CCL17 is required for signaling through CCR4 and establishment of localized inflammatory conditions in the lung.
    Authors: Santulli-Marotto S, Boakye K, Lacy E, Wu S, Luongo J, Kavalkovich K, Coelho A, Hogaboam C, Ryan M
    PLoS ONE, 2013-12-05;8(12):e81465.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Direct ELISA, Neutralization
  11. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012-01-25;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  12. CCL17 controls mast cells for the defense against filarial larval entry.
    Authors: Specht S, Frank JK, Alferink J, Dubben B, Layland LE, Denece G, Bain O, Forster I, Kirschning CJ, Martin C, Hoerauf A
    J. Immunol., 2011-03-11;186(8):4845-52.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  13. Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway.
    Authors: Kearley J, Buckland KF, Mathie SA, Lloyd CM
    Am. J. Respir. Crit. Care Med., 2009-01-29;179(9):772-81.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  14. IL-12 produced by dendritic cells augments CD8+ T cell activation through the production of the chemokines CCL1 and CCL17.
    Authors: Henry CJ, Ornelles DA, Mitchell LM, Brzoza-Lewis KL, Hiltbold EM
    J. Immunol., 2008-12-15;181(12):8576-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  15. CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling.
    Authors: Kearley J, Robinson DS, Lloyd CM
    J. Allergy Clin. Immunol., 2008-07-30;122(3):617-24.e6.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development
  16. iNKT cells require CCR4 to localize to the airways and to induce airway hyperreactivity.
    Authors: Meyer EH, Wurbel MA, Staton TL, Pichavant M, Kan MJ, Savage PB, DeKruyff RH, Butcher EC, Campbell JJ, Umetsu DT
    J. Immunol., 2007-10-01;179(7):4661-71.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  17. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
    Authors: Fujita H, Asahina A, Komine M, Tamaki K
    Cell. Immunol., 2007-05-15;245(2):70-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  18. 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
    Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
    Blood, 2004-05-13;104(5):1404-10.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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Mouse CCL17/TARC Antibody
By Katie Myers on 11/28/2022
Application: capture IgG Sample Tested: Serum Species: Mouse
Other Mouse CCL17/TARC Antibody MAB529