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Human/Mouse IL-5 Antibody

Catalog #: MAB405
15 Citations 2 Reviews Datasheet
Catalog # Availability Size / Price Qty
MAB405-SP
MAB405-100
MAB405-500
Detection of IL‑5 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (15)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse IL-5 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse IL-5 in ELISAs. In sandwich immunoassays with BAM6051 as the detection antibody, no cross-reactivity with recombinant human (rh) IL-3, rhGM-CSF, or recombinant mouse (rm) IL-5 is observed. In sandwich ELISAs with BAM705 as the detection antibody, no cross-reactivity with rhIL-5, rmIL-3, or rmGM-CSF is observed.
Source
Monoclonal Rat IgG1 Clone # TRFK5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse T cell clone-derived partially purified recombinant mouse IL-5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-reported
This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Mouse IL-5 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse IL‑5 Biotinylated Antibody (Catalog # BAM705)

Standard: Recombinant Mouse IL-5 Protein (Catalog # 405-ML)

Human IL-5 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human IL‑5 Biotinylated Antibody (Catalog # BAM6051)

Standard: Recombinant Human IL-5 Protein (Catalog # 205-IL)

Neutralization
Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.004-0.015 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑5.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL-5 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of IL‑5 in Human PBMCs by Flow Cytometry. Human peripheral blood monocytes treated with 50 ng/mL PMA and 500 ng/mL Calcium Ionomycin for 6 hours were stained with Rat Anti-Human/Mouse IL-5 Monoclonal Antibody (Catalog # MAB405) followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B) and Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # MAB005). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Neutralization Cell Proliferation Induced by IL‑5 and Neutralization by Human/Mouse IL‑5 Antibody. View Larger

Cell Proliferation Induced by IL‑5 and Neutralization by Human/Mouse IL‑5 Antibody. Recombinant Mouse IL-5 (Catalog # 405-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human/Mouse IL-5 Monoclonal Antibody (Catalog # MAB405). The ND50 is typically 0.004-0.015 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-5

IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5 specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.

Long Name
Interleukin 5
Entrez Gene IDs
3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
Alternate Names
BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I

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Citations for Human/Mouse IL-5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Time-dependent cell-state selection identifies transiently expressed genes regulating ILC2 activation
    Authors: Yumiko Tanaka, Mai Yamagishi, Yasutaka Motomura, Takashi Kamatani, Yusuke Oguchi, Nobutake Suzuki et al.
    Commun Biol
  2. Annexin A1 is a cell-intrinsic metalloregulator of zinc in human ILC2s
    Authors: Irie, M;Kabata, H;Sasahara, K;Kurihara, M;Shirasaki, Y;Kamatani, T;Baba, R;Matsusaka, M;Koga, S;Masaki, K;Miyata, J;Araki, Y;Kikawada, T;Kabe, Y;Suematsu, M;Yamagishi, M;Uemura, S;Moro, K;Fukunaga, K;
    Cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunoassay
  3. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012-01-25;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  4. Eosinophils promote resolution of acute peritonitis by producing proresolving mediators in mice.
    Authors: Yamada T, Tani Y, Nakanishi H
    FASEB J., 2010-10-19;25(2):561-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  5. Evidence that 13-14 di-hydro, 15-keto prostaglandin D(2)-induced airway eosinophilia in guinea-pigs is independent of interleukin-5.
    Inflamm. Res., 2009-02-01;58(2):103-8.
    Species: Guinea Pig
    Sample Types: In Vivo
    Applications: Neutralization
  6. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
    Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
    Clin. Chem., 2007-11-01;53(11):2010-2.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  7. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007-10-22;329(1):214-8.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  8. Ectopic lung transplantation induces the accumulation of eosinophil progenitors in the recipients' lungs through an allergen- and interleukin-5-dependent mechanism.
    Authors: Xavier-Elsas P, Santos-Maximiano E, Queto T, Mendonca-Sales S, Joseph D, Gaspar-Elsas MI, Vargaftig BB
    Clin. Exp. Allergy, 2007-01-01;37(1):29-38.
    Species: Mouse
    Sample Types: Peritoneal Fluid
    Applications: ELISA Development
  9. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006-06-16;120(2):220-31.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  10. Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
    Authors: Wen H, Hogaboam CM, Gauldie J, Kunkel SL
    Am. J. Pathol., 2006-06-01;168(6):1940-50.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  11. The IgE adjuvant effect of particles: characterisation of the primary cellular response in the draining lymph node.
    Authors: Nygaard UC, Ormstad H, Aase A, Lovik M
    Toxicology, 2005-01-15;206(2):181-93.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  12. Arthropod-derived histamine-binding protein prevents murine allergic asthma.
    Authors: Couillin I, Maillet I, Jacobs M, Paesen GC, Moser R, Weston-Davies W
    J. Immunol., 2004-09-01;173(5):3281-6.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development
  13. Marked airway eosinophilia prevents development of airway hyper-responsiveness during an allergic response in IL-5 transgenic mice.
    Authors: Kobayashi T, Iijima K, Kita H
    J. Immunol., 2003-06-01;170(11):5756-63.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  14. AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
    Authors: Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ
    Am. J. Pathol., 2002-04-01;160(4):1353-60.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  15. Comparison of effects of anti-IL-3, IL-5 and GM-CSF treatments on eosinophilopoiesis and airway eosinophilia induced by allergen.
    Authors: Tomaki M, Zhao LL, Sjostrand M, Linden A, Ichinose M, Lotvall J
    Pulm Pharmacol Ther, 2002-01-01;15(2):161-8.
    Species: Mouse
    Sample Types:
    Applications: Neutralization

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Reviews for Human/Mouse IL-5 Antibody

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Human/Mouse IL-5 Antibody
By Anonymous on 09/18/2019
Application: Func Sample Tested: IEC-18 rat small intestinal epithelial cell line Species: Rat
Human/Mouse IL-5 Antibody
By Leslie Priddy on 04/01/2018
Application: Flow Sample Tested: Tumor cell lyastes Species: Mouse