Recombinant Mouse IL-5 Protein Summary
Product Specifications
Met21-Gly133
Analysis
Product Datasheets
405-ML (with carrier)
405-ML/CF (carrier free)
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
405-ML
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Reconstitution | Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
405-ML/CF
Formulation | Supplied as a 0.2 μm filtered solution in PBS. |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IL-5
Interleukin-5 (IL-5) is a secreted glycoprotein that belongs to the alpha -helical group of cytokines (1 ‑ 3). Unlike other family members, it is present as a covalently linked antiparallel dimer (4, 5). The cDNA for mouse IL‑5 encodes a signal peptide and a 113 amino acid (aa) mature protein. Mature mouse IL‑5 shares 70%, 94%, 58%, 66%, 59% and 63%, aa sequence identity with human, rat, canine, equine, feline and porcine IL-5, respectively, and shows cross-reactivity with human IL-5 receptor. IL-5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in Hodgkin’s disease, and IL-2-stimulated invariant natural killer T cells (iNKT) (1 ‑ 3, 6 ‑ 8). IL-5 increases production and mobilization of eosinophils and CD34+ progenitors from the bone marrow and causes maturation of eosinophil precursors outside the bone marrow (1, 6, 9, 10). The receptor for human IL-5, mainly expressed by eosinophils, but also found on basophils and mast cells, consists of a unique ligand-binding subunit (IL-5 R alpha ) and a shared signal‑transducing subunit, beta c (3, 6, 11). IL-5 R alpha first binds IL-5 at low affinity, then associates with preformed beta c dimers, forming a high-affinity receptor (12). IL-5 also binds proteoglycans, potentially enhancing its activity (13). Soluble forms of IL-5 R alpha antagonize IL-5 and can be found in vivo (10, 14). In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells (1 ‑ 3, 9, 10, 15, 16). IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release (17).
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- Kopf, M. et al. (1996) Immunity 4:15.
- Horikawa, K. and K. Takatsu (2006) Immunology 118:497.
- Denburg, J. A. et al. (1991) Blood 77:1462.
Citations for Recombinant Mouse IL-5 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Regulatory T-cells and IL-5 mediate pain outcomes in a preclinical model chronic muscle pain
Authors: ME Lenert, TA Szabo-Pard, MDD Burton
Oncogene, 2023-01-01;0(0):1744806922111.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Non-redundant functions of group 2 innate lymphoid cells
Authors: KJ Jarick, PM Topczewska, MO Jakob, H Yano, M Arifuzzama, X Gao, S Boulekou, V Stokic-Trt, PS Leclère, A Preu beta er, ZA Rompe, A Stamm, AM Tsou, C Chu, FR Heinrich, GM Guerra, P Durek, A Ivanov, D Beule, S Helfrich, CU Duerr, AA Kühl, C Stehle, C Romagnani, MF Mashreghi, A Diefenbach, D Artis, CSN Klose
Nature, 2022-11-02;0(0):.
Species: Transgenic Mouse
Sample Types: In Vivo
Applications: In Vivo -
Phosphatidylinositol 3-Kinase (PI3K) Orchestrates Aspergillus fumigatus-Induced Eosinophil Activation Independently of Canonical Toll-Like Receptor (TLR)/C-Type-Lectin Receptor (CLR) Signaling
Authors: A Dietschman, S Schruefer, S Westermann, F Henkel, K Castiglion, R Willebrand, J Adam, J Ruland, R Lang, DC Sheppard, J Esser-von-, D Radtke, S Krappmann, D Voehringer
MBio, 2022-06-13;0(0):e0123922.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Targeting BMI-1 in B cells restores effective humoral immune responses and controls chronic viral infection
Authors: A Di Pietro, J Polmear, L Cooper, T Damelang, T Hussain, L Hailes, K O'Donnell, V Udupa, T Mi, S Preston, A Shtewe, U Hershberg, SJ Turner, NL La Gruta, AW Chung, DM Tarlinton, CD Scharer, KL Good-Jacob
Nature Immunology, 2021-11-29;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Fam72a enforces error-prone DNA repair during antibody diversification
Authors: M Rogier, J Moritz, I Robert, C Lescale, V Heyer, A Abello, O Martin, K Capitani, M Thomas, AS Thomas-Cla, B Laffleur, F Jouan, E Pinaud, K Tarte, M Cogné, SG Conticello, E Soutoglou, L Deriano, B Reina-San-
Nature, 2021-11-24;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Monoacylglycerol lipase deficiency in the tumor microenvironment slows tumor growth in non-small cell lung cancer.
Authors: Kienzl M, Hasenoehrl C, Maitz K, Sarsembayeva A, Taschler U, Valadez-Cosmes P, Kindler O, Ristic D, Raftopoulou S, Santiso A, Barnthaler T, Brcic L, Hahnefeld L, Gurke R, Thomas D, Geisslinger G, Kargl J, Schicho R
Oncoimmunology, 2021-09-11;10(1):1965319.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Airway-Associated Macrophages in Homeostasis and Repair
Authors: AE Engler, AB Ysasi, RMF Pihl, C Villacorta, HM Heston, HMK Richardson, BR Thapa, NR Moniz, AC Belkina, SA Mazzilli, JR Rock
Cell Reports, 2020-12-29;33(13):108553.
Species: Transgenic Mouse
Sample Types: Spheroid
Applications: Bioassay -
Chromatin Protein PC4 Orchestrates B Cell Differentiation by Collaborating with IKAROS and IRF4
Authors: K Ochiai, M Yamaoka, A Swaminatha, H Shima, H Hiura, M Matsumoto, D Kurotaki, J Nakabayash, R Funayama, K Nakayama, T Arima, T Ikawa, T Tamura, R Sciammas, P Bouvet, TK Kundu, K Igarashi
Cell Reports, 2020-12-22;33(12):108517.
Species: Mouse, Transgenic Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Eosinophils improve cardiac function after myocardial infarction
Authors: J Liu, C Yang, T Liu, Z Deng, W Fang, X Zhang, J Li, Q Huang, C Liu, Y Wang, D Yang, GK Sukhova, JS Lindholt, A Diederichs, LM Rasmussen, D Li, G Newton, FW Luscinskas, L Liu, P Libby, J Wang, J Guo, GP Shi
Nature Communications, 2020-12-16;11(1):6396.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
The Histone Methyltransferase DOT1L Is Essential for Humoral Immune Responses
Authors: L Kealy, A Di Pietro, L Hailes, S Scheer, L Dalit, JR Groom, C Zaph, KL Good-Jacob
Cell Reports, 2020-12-15;33(11):108504.
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Bioassay -
An Erg-driven transcriptional program controls B cell lymphopoiesis
Authors: AP Ng, HD Coughlan, S Hediyeh-Za, K Behrens, TM Johanson, MSY Low, CC Bell, O Gilan, YC Chan, AJ Kueh, T Boudier, R Feltham, A Gabrielyan, L DiRago, CD Hyland, H Ierino, S Mifsud, E Viney, T Willson, MA Dawson, RS Allan, MJ Herold, K Rogers, DM Tarlinton, GK Smyth, MJ Davis, SL Nutt, WS Alexander
Nat Commun, 2020-06-15;11(1):3013.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
B cell Sirt1 deacetylates histone and non-histone proteins for epigenetic modulation of AID expression and the antibody response
Authors: H Gan, T Shen, DP Chupp, JR Taylor, HN Sanchez, X Li, Z Xu, H Zan, P Casali
Sci Adv, 2020-04-01;6(14):eaay2793.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Microbiota-driven interleukin-17-producing cells and eosinophils synergize to accelerate multiple myeloma progression
Authors: A Calcinotto, A Brevi, M Chesi, R Ferrarese, L Garcia Per, M Grioni, S Kumar, VM Garbitt, ME Sharik, KJ Henderson, G Tonon, M Tomura, Y Miwa, E Esplugues, RA Flavell, S Huber, F Canducci, VS Rajkumar, PL Bergsagel, M Bellone
Nat Commun, 2018-12-03;9(1):4832.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Murine eosinophil development and allergic lung eosinophilia are largely dependent on the signaling adaptor GRB2
Authors: R Willebrand, A Dietschman, L Nitschke, S Krappmann, D Voehringer
Eur. J. Immunol., 2018-09-06;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Mining the Plasma Cell Transcriptome for Novel Cell Surface Proteins
Authors: S Trezise, A Karnowski, PL Fedele, S Mithraprab, Y Liao, K D'Costa, AJ Kueh, MP Hardy, CM Owczarek, MJ Herold, A Spencer, W Shi, SN Willis, SL Nutt, LM Corcoran
Int J Mol Sci, 2018-07-24;19(8):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
B cell activation and plasma cell differentiation are inhibited by de novo DNA methylation
Authors: BG Barwick, CD Scharer, RJ Martinez, MJ Price, AN Wein, RR Haines, APR Bally, JE Kohlmeier, JM Boss
Nat Commun, 2018-05-15;9(1):1900.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
mTOR complexes differentially orchestrates eosinophil development in allergy
Authors: C Zhu, L Xia, F Li, L Zhou, Q Weng, Z Li, Y Wu, Y Mao, C Zhang, Y Wu, M Li, S Ying, Z Chen, H Shen, W Li
Sci Rep, 2018-05-02;8(1):6883.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Plasma cell differentiation is controlled by multiple cell division-coupled epigenetic programs
Authors: CD Scharer, BG Barwick, M Guo, APR Bally, JM Boss
Nat Commun, 2018-04-27;9(1):1698.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The Esophageal Organoid System Reveals Functional Interplay Between Notch and Cytokines in Reactive Epithelial�Changes
Authors: Y Kasagi, PM Chandramou, KA Whelan, K Tanaka, V Giroux, M Sharma, J Wang, AJ Benitez, M DeMarshall, JW Tobias, KE Hamilton, GW Falk, JM Spergel, AJ Klein-Szan, AK Rustgi, AB Muir, H Nakagawa
Cell Mol Gastroenterol Hepatol, 2018-01-03;5(3):333-352.
Species: Mouse
Sample Types: Organoids
Applications: Bioassay -
Elevating adipose eosinophils in obese mice to physiologically normal levels does not rescue metabolic impairments
Authors: WR Bolus, KR Peterson, MJ Hubler, AJ Kennedy, ML Gruen, AH Hasty
Mol Metab, 2017-12-16;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
The Small Rho GTPase TC10 Modulates B Cell Immune Responses
Authors: M Burbage, SJ Keppler, B Montaner, PK Mattila, FD Batista
J. Immunol., 2017-07-26;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
IL33-mediated ILC2 activation and neutrophil IL5 production in the lung response after severe trauma: A reverse translation study from a human cohort to a mouse trauma model
Authors: J Xu, J Guardado, R Hoffman, H Xu, R Namas, Y Vodovotz, L Xu, M Ramadan, J Brown, HR Turnquist, TR Billiar
PLoS Med., 2017-07-25;14(7):e1002365.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Rad52 competes with Ku70/Ku86 for binding to S-region DSB ends to modulate antibody class-switch DNA recombination
Authors: H Zan, C Tat, Z Qiu, JR Taylor, JA Guerrero, T Shen, P Casali
Nat Commun, 2017-02-08;8(0):14244.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3(+)CD49b(-)Foxp3(-) regulatory T�cells
Authors: W Dawicki, C Li, J Town, X Zhang, JR Gordon
J. Allergy Clin. Immunol., 2016-10-26;139(5):1608-1620.e3.
Species: Mouse
Sample Types: Serum
Applications: ELISA (Standard) -
Eosinophil differentiation in the bone marrow is promoted by protein tyrosine phosphatase SHP2
Authors: LX Xia, W Hua, Y Jin, BP Tian, ZW Qiu, C Zhang, LQ Che, HB Zhou, YF Wu, HQ Huang, F Lan, YH Ke, JJ Lee, W Li, SM Ying, ZH Chen, HH Shen
Cell Death Dis, 2016-04-07;7(0):e2175.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Eosinophil-specific deletion of IkappaBalpha in mice reveals a critical role of NF-kappaB-induced Bcl-xL for inhibition of apoptosis.
Authors: Schwartz C, Willebrand R, Huber S, Rupec R, Wu D, Locksley R, Voehringer D
Blood, 2015-04-10;125(25):3896-904.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
IL-25 inhibits atherosclerosis development in apolipoprotein E deficient mice.
Authors: Mantani P, Duner P, Bengtsson E, Alm R, Ljungcrantz I, Soderberg I, Sundius L, To F, Nilsson J, Bjorkbacka H, Fredrikson G
PLoS ONE, 2015-01-28;10(1):e0117255.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
The generation of neutrophils in the bone marrow is controlled by autophagy.
Authors: Rozman S, Yousefi S, Oberson K, Kaufmann T, Benarafa C, Simon H
Cell Death Differ, 2014-10-17;22(3):445-56.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Bcl-xL protein protects from C/EBP homologous protein (CHOP)-dependent apoptosis during plasma cell differentiation.
Authors: Gaudette B, Iwakoshi N, Boise L
J Biol Chem, 2014-07-14;289(34):23629-40.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Expression profiling of differentiating eosinophils in bone marrow cultures predicts functional links between microRNAs and their target mRNAs.
Authors: Yang M, Eyers F, Xiang Y, Guo M, Young I, Rosenberg H, Foster P
PLoS ONE, 2014-05-13;9(5):e97537.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Alternative splice variants of AID are not stoichiometrically present at the protein level in chronic lymphocytic leukemia.
Authors: Rebhandl S, Huemer M, Zaborsky N, Gassner F, Catakovic K, Felder T, Greil R, Geisberger R
Eur J Immunol, 2014-04-15;44(7):2175-87.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Basophils regulate the recruitment of eosinophils in a murine model of irritant contact dermatitis.
Authors: Nakashima C, Otsuka A, Kitoh A, Honda T, Egawa G, Nakajima S, Nakamizo S, Arita M, Kubo M, Miyachi Y, Kabashima K
J Allergy Clin Immunol, 2014-04-06;134(1):100-7.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Follicular dendritic cell secreted protein FDC-SP controls IgA production.
Authors: Hou S, Landego I, Jayachandran N, Miller A, Gibson I, Ambrose C, Marshall A
Mucosal Immunol, 2014-01-08;7(4):948-57.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity.
Authors: Palm N, Rosenstein R, Yu S, Schenten D, Florsheim E, Medzhitov R
Immunity, 2013-10-24;39(5):976-85.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Homologous recombination into the eosinophil peroxidase locus generates a strain of mice expressing Cre recombinase exclusively in eosinophils.
Authors: Doyle, Alfred D, Jacobsen, Elizabet, Ochkur, Sergei I, Willetts, Lian, Shim, Kelly, Neely, Joseph, Kloeber, Jake, Lesuer, Will E, Pero, Ralph S, Lacy, Paige, Moqbel, Redwan, Lee, Nancy A, Lee, James J
J Leukoc Biol, 2013-04-29;94(1):17-24.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Regulation of innate CD8+ T-cell activation mediated by cytokines.
Proc Natl Acad Sci U S A, 2012-06-04;109(25):9971-6.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Innate lymphoid cells promote lung-tissue homeostasis after infection with influenza virus.
Authors: Monticelli LA, Sonnenberg GF, Abt MC
Nat. Immunol., 2011-11-01;12(11):1045-54.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Defective eosinophil hematopoiesis ex vivo in inbred Rocky Mountain White (IRW) mice.
Authors: Dyer KD, Garcia-Crespo KE, Percopo CM, Bowen AB, Ito T, Peterson KE, Gilfillan AM, Rosenberg HF
J. Leukoc. Biol., 2011-08-30;90(6):1101-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Impaired basophil induction leads to an age-dependent innate defect in type 2 immunity during helminth infection in mice.
Authors: Nel HJ, Hams E, Saunders SP
J. Immunol., 2011-03-11;186(8):4631-9.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus.
Authors: Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S
J. Virol., 2010-09-29;84(24):12703-12.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut.
Authors: Suzuki K, Maruya M, Kawamoto S
Immunity, 2010-07-23;33(1):71-83.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Sphingosine 1-phosphate regulates the egress of IgA plasmablasts from Peyer's patches for intestinal IgA responses.
Authors: Gohda M, Kunisawa J, Miura F, Kagiyama Y, Kurashima Y, Higuchi M, Ishikawa I, Ogahara I, Kiyono H
J. Immunol., 2008-04-15;180(8):5335-43.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
B7-DC induced by IL-13 works as a feedback regulator in the effector phase of allergic asthma.
Authors: Matsumoto K, Fukuyama S, Eguchi-Tsuda M, Nakano T, Matsumoto T, Matsumura M, Moriwaki A, Kan-o K, Wada Y, Yagita H, Shin T, Pardoll DM, Patcharee R, Azuma M, Nakanishi Y, Inoue H
Biochem. Biophys. Res. Commun., 2007-11-05;365(1):170-5.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
Authors: Wen H, Hogaboam CM, Gauldie J, Kunkel SL
Am. J. Pathol., 2006-06-01;168(6):1940-50.
Applications: ELISA (Standard) -
The IgE adjuvant effect of particles: characterisation of the primary cellular response in the draining lymph node.
Authors: Nygaard UC, Ormstad H, Aase A, Lovik M
Toxicology, 2005-01-15;206(2):181-93.
Applications: ELISA (Standard) -
Arthropod-derived histamine-binding protein prevents murine allergic asthma.
Authors: Couillin I, Maillet I, Jacobs M, Paesen GC, Moser R, Weston-Davies W
J. Immunol., 2004-09-01;173(5):3281-6.
Applications: ELISA (Standard) -
Marked airway eosinophilia prevents development of airway hyper-responsiveness during an allergic response in IL-5 transgenic mice.
Authors: Kobayashi T, Iijima K, Kita H
J. Immunol., 2003-06-01;170(11):5756-63.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The effects of intranasal budesonide on allergen-induced production of interleukin-5 and eotaxin, airways, blood, and bone marrow eosinophilia, and eosinophil progenitor expansion in sensitized mice.
Authors: O'Byrne PM
Am. J. Respir. Crit. Care Med., 2002-07-15;166(2):146-53.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
Authors: Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ
Am. J. Pathol., 2002-04-01;160(4):1353-60.
Applications: ELISA (Standard)
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