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Mouse VEGFR3/Flt-4 Biotinylated Antibody

Catalog #: BAF743
4 Citations Datasheet
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BAF743
Product Details
Citations (4)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse VEGFR3/Flt-4 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse VEGFR3 in ELISAs and Western blots. In sandwich ELISAs, less than 7% cross-reactivity with recombinant human VEGFR3 is observed and 0.2% cross-reactivity with recombinant mouse (rm) VEGFR1 and rmVEGFR2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse VEGFR3/Flt-4
Tyr25-Asp770
Accession # P35917
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse VEGFR3/Flt‑4 Fc Chimera (Catalog # 743-R3)
Flow Cytometry
2.5 µg/106 cells
MS‑1 mouse pancreatic islet endothelial cell line

Mouse VEGFR3/Flt-4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse VEGFR3/Flt-4 Antibody (Catalog # MAB7431)

Standard: Recombinant Mouse VEGFR3/Flt-4 Fc Chimera Protein, CF (Catalog # 743-R3)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR3/Flt-4

VEGFR3 (Flt-4), together with VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1), belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of these receptors is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

In adults, VEGFR3 expression is restricted to the endothelial cells of the lymphatic vessels. Mouse VEGFR3 cDNA encodes a 1363 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGFR3 has a 751 aa residue extracellular domain, a 22 aa residue hydrophobic transmembrane domain, and a 565 aa residue cytoplasmic domain. The polypeptide sequences of murine Flt-4 is 88% identical to the human homologue. VEGFR3 has been reported to serve as the receptors for VEGF-C and VEGF-D.

References
  1. Finnerty, H. et al. (1993) Oncogene 8:2293.
  2. Joukov, V. et al. (1996) EMBO J. 15:290.
  3. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
Long Name
Vascular Endothelial Growth Factor Receptor 3
Entrez Gene IDs
2324 (Human); 14257 (Mouse)
Alternate Names
EC 2.7.10; EC 2.7.10.1; FLT4; Flt-4; fms-related tyrosine kinase 4; LMPH1A; PCLFLT41; soluble VEGFR3 variant 1; soluble VEGFR3 variant 2; soluble VEGFR3 variant 3; Tyrosine-protein kinase receptor FLT4; vascular endothelial growth factor receptor 3; VEGF R3; VEGFR3; VEGFR-3; VEGFR3Fms-like tyrosine kinase 4

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Citations for Mouse VEGFR3/Flt-4 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Complementary Wnt Sources Regulate Lymphatic Vascular Development via PROX1-Dependent Wnt/?-Catenin Signaling
    Authors: B Cha, X Geng, MR Mahamud, JY Zhang, L Chen, W Kim, EH Jho, Y Kim, D Choi, JB Dixon, H Chen, YK Hong, L Olson, TH Kim, BJ Merrill, MJ Davis, RS Srinivasan
    Cell Rep, 2018-10-16;25(3):571-584.e5.
    Species: Mouse
    Sample Types: Whole Tissue
  2. Mesenchymal Stromal Cells Inhibit Inflammatory Lymphangiogenesis in the Cornea by Suppressing Macrophage in a TSG-6-Dependent Manner
    Authors: HB Song, SY Park, JH Ko, JW Park, CH Yoon, DH Kim, JH Kim, MK Kim, RH Lee, DJ Prockop, JY Oh
    Mol. Ther., 2017-10-05;26(1):162-172.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Normal lymphatic development and function in mice deficient for the lymphatic hyaluronan receptor LYVE-1.
    Authors: Gale NW, Prevo R, Espinosa J, Ferguson DJ, Dominguez MG, Yancopoulos GD, Thurston G, Jackson DG
    Mol. Cell. Biol., 2006-11-13;27(2):595-604.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Endothelial VEGFR Coreceptors Neuropilin-1 and Neuropilin-2 Are Essential for Tumor Angiogenesis
    Authors: Christopher J. Benwell, Robert T. Johnson, James A.G.E. Taylor, Christopher A. Price, Stephen D. Robinson
    Cancer Research Communications

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