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Recombinant Mouse VEGFR3/Flt-4 Fc Chimera Protein, CF

Catalog #: 743-R3
9 Citations Datasheet
Catalog # Availability Size / Price Qty
743-R3-100
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Citations (9)
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Recombinant Mouse VEGFR3/Flt-4 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse VEGFR3/Flt-4 Fc Chimera (Catalog # 743-R3) at 5 μg/mL (100 μL/well) can bind Recombinant Mouse VEGF‑D (Catalog # 469-VD) with an apparent Kd <50 nM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse VEGFR3/Flt-4 protein
MetMouse VEGFR3
(Tyr25-Asp770)
Accession # P35917		IEGRDIDHuman IgG1
(Pro100-Lys330)
6-His tag
N-terminusC-terminus
Accession #
P35917
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
112 kDa (monomer)
SDS-PAGE
123 kDa with two additional minor bands at 73 kDa and 60 kDa, reducing conditions

Product Datasheets

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743-R3

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

743-R3

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR3/Flt-4

VEGFR3 (Flt-4), together with VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1), belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of these receptors is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

In adults, VEGFR3 expression is restricted to the endothelial cells of the lymphatic vessels. Mouse VEGFR3 cDNA encodes a 1363 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGFR3 has a 751 aa residue extracellular domain, a 22 aa residue hydrophobic transmembrane domain and a 565 aa residue cytoplasmic domain. The polypeptide sequences of murine Flt-4 is 88% identical to the human homologue. VEGFR3 has been reported to serve as the receptors for VEGF-C and VEGF-D.

References
  1. Finnerty, H. et al. (1993) Oncogene 8:2293.
  2. Joukov, V. et al. (1996) EMBO J. 15:290.
  3. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA. 95:548.
Long Name
Vascular Endothelial Growth Factor Receptor 3
Entrez Gene IDs
2324 (Human); 14257 (Mouse)
Alternate Names
EC 2.7.10; EC 2.7.10.1; FLT4; Flt-4; fms-related tyrosine kinase 4; LMPH1A; PCLFLT41; soluble VEGFR3 variant 1; soluble VEGFR3 variant 2; soluble VEGFR3 variant 3; Tyrosine-protein kinase receptor FLT4; vascular endothelial growth factor receptor 3; VEGF R3; VEGFR3; VEGFR-3; VEGFR3Fms-like tyrosine kinase 4

Citations for Recombinant Mouse VEGFR3/Flt-4 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Blockade of VEGF-C signaling inhibits lymphatic malformations driven by oncogenic PIK3CA mutation
    Authors: I Martinez-C, Y Zhang, M Petkova, H Ortsäter, S Sjöberg, SD Castillo, P Brouillard, L Libbrecht, D Saur, M Graupera, K Alitalo, L Boon, M Vikkula, T Mäkinen
    Nat Commun, 2020-06-08;11(1):2869.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Western Blot Control
  2. Structural and functional features of central nervous system lymphatic vessels.
    Authors: Louveau A, Smirnov I, Keyes T, Eccles J, Rouhani S, Peske J, Derecki N, Castle D, Mandell J, Lee K, Harris T, Kipnis J
    Nature, 2015-06-01;523(7560):337-41.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Antibody Absorption
  3. Vascular endothelial growth factor c promotes ovarian carcinoma progression through paracrine and autocrine mechanisms.
    Authors: Decio A, Taraboletti G, Patton V, Alzani R, Perego P, Fruscio R, Jurgensmeier J, Giavazzi R, Belotti D
    Am J Pathol, 2014-02-06;184(4):1050-61.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. CD8+ T cells suppress viral replication in the cornea but contribute to VEGF-C-induced lymphatic vessel genesis.
    Authors: Conrady CD, Zheng M, Stone DU
    J. Immunol., 2012-05-30;189(1):425-32.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Notch-dependent VEGFR3 upregulation allows angiogenesis without VEGF-VEGFR2 signalling.
    Authors: Benedito R, Rocha S, Woeste M, Zamykal M, Radtke F, Casanovas O, Duarte A, Pytowski B, Adams R
    Nature, 2012-03-18;484(7392):110-4.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  6. VEGF-A expression by HSV-1-infected cells drives corneal lymphangiogenesis.
    Authors: Wuest TR, Carr DJ
    J. Exp. Med., 2009-12-21;207(1):101-15, S1-2.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. VEGF-A produced by chronically inflamed tissue induces lymphangiogenesis in draining lymph nodes.
    Authors: Halin C, Tobler NE, Vigl B, Brown LF, Detmar M
    Blood, 2007-07-11;110(9):3158-67.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. Nonvascular VEGF receptor 3 expression by corneal epithelium maintains avascularity and vision.
    Authors: Cursiefen C, Chen L, Saint-Geniez M, Hamrah P, Jin Y, Rashid S, Pytowski B, Persaud K, Wu Y, Streilein JW, Dana R
    Proc. Natl. Acad. Sci. U.S.A., 2006-07-18;103(30):11405-10.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  9. Hepatocyte growth factor is a lymphangiogenic factor with an indirect mechanism of action.
    Authors: Cao R, Bjorndahl MA, Gallego MI, Chen S, Religa P, Hansen AJ, Cao Y
    Blood, 2006-01-19;107(9):3531-6.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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