Recombinant Mouse CD117/c-kit Fc Chimera Protein, CF Summary
Product Specifications
| Mouse CD117/c-kit (Gln26-Thr523) Accession # P05532 |
IEGRMDP | Mouse IgG2A (Glu98-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
1356-SR
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1356-SR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: CD117/c-kit
Stem Cell Factor Receptor (SCF R), also known as c‑Kit and CD117, is a widely expressed 145 kDa receptor tyrosine kinase. It is the cellular homolog of the feline sarcoma virus protein, v‑Kit. Binding of SCF R to SCF, also known as Steel Factor and Kit Ligand, promotes the survival, differentiation, and mobilization of progenitor cells in multiple lineages (1‑4). Mutations or deletions of SCF R cause a wide variety of malignancies as well as pigmentation disorders and sterility (5, 6). Mature mouse SCF R consists of a 503 amino acid (aa) extracellular domain (ECD) with five tandem immunoglobulin‑like domains, a 21 aa transmembrane segment, and a 431 aa cytoplasmic domain with the split tyrosine kinase domain (7). Within the ECD, mouse SCF R shares 73% and 88% aa sequence identity with human and rat SCF R, respectively. Alternative splicing of mouse SCF R generates a truncated intracellular isoform that corresponds to the C‑terminal half of the tyrosine kinase domain (8). SCF is expressed as transmembrane and soluble noncovalent homodimers (9). One SCF dimer binds to two molecules of SCF R, inducing receptor dimerization and activation (9). Transmembrane SCF induces more prolonged signaling through SCF R compared to soluble SCF (10). Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (11). A 100 kDa glycosylated ECD fragment of SCF R can be shed into the circulation by TACE/ADAM17, and this fragment inhibits the interaction of SCF with transmembrane SCF R (12, 13). SCF is a primary growth and activation factor for mast cells and eosinophils (14). SCF R expression on mast cells enables them to infiltrate SCF‑secreting tumors where they promote tumor growth and induce local immune suppression (15). SCF R is up‑regulated on dendritic cells by Th2‑ or Th17‑biasing stimuli, and it is required for subsequent dendritic cell induction of Th2 and Th17 responses (16). SCF R protects vascular smooth muscle cells from apoptosis and assists in the recovery of cardiac function following myocardial infarction (17, 18).
- Kimura, Y. et al. (2011) PLoS ONE 6:e26918.
- Tallini, Y.N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:1808.
- Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039.
- Sun, L. et al. (2004) J. Clin. Invest. 113:1364.
- Pittoni, P. et al. (2011) Oncogene 30:757.
- Sartini, S. et al. (2011) Curr. Med. Chem. 18:2893.
- Qiu, F.H. et al. (1988) EMBO J. 7:1003.
- Zayas, J. et al. (2008) Stem Cells Dev. 17:343.
- Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
- Miyazawa, K. et al. (1995) Blood 85:641.
- Martin, F.H. et al. (1990) Cell 63:203.
- Turner, A.M. et al. (1995) Blood 85:2052.
- Cruz, A.C. et al. (2004) J. Biol. Chem. 279:5612.
- Jamur, M.C. and C. Oliver (2011) Front. Biosci. (School Ed.) 3:1390.
- Huang, B. et al. (2008) Blood 112:1269.
- Krishnamoorthy, N. et al. (2008) Nat. Med. 14:565.
- Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
- Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.
Citation for Recombinant Mouse CD117/c-kit Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Paneth Cells Respond to Inflammation and Contribute to Tissue Regeneration by Acquiring Stem-like Features through SCF/c-Kit Signaling
Authors: M Schmitt, M Schewe, A Sacchetti, D Feijtel, WS van de Gee, M Teeuwssen, HF Sleddens, R Joosten, ME van Royen, HJG van de Wer, J van Es, H Clevers, R Fodde
Cell Rep, 2018-08-28;24(9):2312-2328.e7.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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