Recombinant Human CD117/c-kit His-tag Avi-tag Protein, CF

Catalog #: AVI10931 Datasheet

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI10931-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD117/c-kit His-tag Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human SCF (Catalog # 7644-SC) is coated at 1.00 μg/mL, 100 μL/well, Biotinylated Recombinant Human CD117/c-kit His-tag Avi-tag binds with an ED₅₀ of 0.600-3.60 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human CD117/c-kit protein

Human CD117/c-kit (Gln26-Thr520) Accession # P10721.1	HHHHHH	Avi-tag
N-terminus		C-terminus

Accession #

P10721.1

N-terminal Sequence
Analysis

Protein identity confirmed by mass spectrometry.

Structure / Form

Biotinylated via Avi-tag.

Predicted Molecular Mass

58 kDa

SDS-PAGE

85-105 kDa, under reducing conditions.

Product Datasheets

AVI10931

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10931

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human SCF (7644-SC) is coated at 1.00 ug/mL, 100 uL/well, Biotinylated Recombinant Human CD117/c-kit His-tag Avi-tag (Catalog # AVI10931) binds with an ED₅₀ of 0.6-3.6 ug/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human CD117/c-kit His-tag Avi-tag Protein (Catalog # AVI10931) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-105 kDa.

Background: CD117/c-kit

Stem cell factor receptor (SCFR), also known as c-Kit and CD117, is a type III receptor tyrosine kinase that acts as the receptor for the cytokine SCF (1, 2). Mature human SCFR consists of an extracellular domain (ECD) with five tandem immunoglobulin-like domains, a single transmembrane segment, and a cytoplasmic region with a split tyrosine kinase domain. Within the ECD, human SCFR shares 73% and 76% amino acid sequence identity with mouse and rat SCFR, respectively. At least 4 SCFR isoforms, produced by alternative mRNA splicing, have been identified in humans and 2 are characterized by the presence or absence of a tetrapeptide sequence (GNNK) in the juxtamembrane region of the ECD (3, 4). Human SCFR is expressed in embryonic stem cells and is involved in regulation of cell survival and proliferation, hematopoiesis, gametogenesis, and melanogenesis (1, 2, 5, 6). SCF R interacts with noncovalent dimers of transmembrane or soluble SCF to trigger receptor dimerization and initiate signaling (7). Our Avi-tag Biotinylated Recombinant Human SCFR features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Roskoski, R. et al. (2005) Bioche. & Biophy Res Comm. 338:1307.
Ashman, L.K. et al. (1999) Inter Jour of Biochem & Cell Bio. 31:1037.
Lennartsson, J. and Rönnstrand L. (2012) Physiol Rev. 92:1619.
Caruana, G. et al. (1999) Oncogene 18:5573.
Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201.
Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.

Long Name

Stem Cell Factor Receptor

Entrez Gene IDs

3815 (Human); 16590 (Mouse); 64030 (Rat)

Alternate Names

CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFR; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

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