Home / CD117/c-kit / Recombinant Human CD117/c-kit Fc Chimera Avi-tag Protein, CF

Recombinant Human CD117/c-kit Fc Chimera Avi-tag Protein, CF

Catalog #: AVI10703 Datasheet
Catalog # Availability Size / Price Qty
AVI10703-050
Biotinylated Recombinant Human CD117/c-kit Fc Chimera Avi-tag Binding Activity.
2 Images
Product Details
FAQs
Supplemental Products
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD117/c-kit Fc Chimera Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human SCF (Mammalian-expressed, Catalog # 7644-SC) is coated at 1 μg/mL, 100 μL/well, Biotinylated Recombinant Human CD117/c-kit Fc Chimera Avi-tag binds with an ED50 of 0.05-0.30   μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human CD117/c-kit protein
Human CD117/c-kit
(Gln26-Thr520)
Accession # P10721.1		IEGRMDHuman IgG1
(Pro100-Lys330)		Avi-tag
N-terminusC-terminus
Accession #
P10721.1
N-terminal Sequence
Analysis
Gln26 (Blocked)
Structure / Form
Disulfide-linked homodimer, biotinylated via Avi-tag
Predicted Molecular Mass
84 kDa
SDS-PAGE
100-120 kDa, under reducing conditions

Product Datasheets

You must select a language.

x

AVI10703

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10703

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant Human SCF (Mammalian-expressed) Protein (7466-SC) is coated at 1 ug/mL, 100 uL/well, Biotinylated Recombinant Human CD117/c-kit Fc Chimera Avi-tag binds with an ED50 of 0.05-0.30 μg/mL.

SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human CD117/c-kit Fc Chimera Avi-tag (Catalog # AVI10703) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 100-120 kDa and 200-240 kDa, respectively.

Background: CD117/c-kit

Stem cell factor receptor (SCF R), also known as c-Kit and CD117, is a type III receptor tyrosine kinase that acts as the receptor for the cytokine SCF (1, 2). Mature human SCF R consists of an extracellular domain (ECD) with five tandem immunoglobulin-like domains, a single transmembrane segment, and a cytoplasmic region with a split tyrosine kinase domain. Within the ECD, human SCF R shares 73% and 76% amino acid sequence identity with mouse and rat SCF R, respectively. At least 4 SCF R isoforms, produced by alternative mRNA splicing, have been identified in humans and 2 are characterized by the presence or absence of a tetrapeptide sequence (GNNK) in the juxtamembrane region of the ECD (3, 4). Human SCF R is expressed in embryonic stem cells and is involved in regulation of cell survival and proliferation, hematopoiesis, gametogenesis, and melanogenesis (1, 2, 5, 6). SCF R interacts with noncovalent dimers of transmembrane or soluble SCF to trigger receptor dimerization and initiate signaling (7). Our Avi-tag Biotinylated rhSCF R features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References
  1. Roskoski, R. et al. (2005) Bioche. & Biophy Res Comm. 338:1307.
  2. Ashman, L.K. et al. (1999) Inter Jour of Biochem & Cell Bio. 31:1037.
  3. Lennartsson J. and Rönnstrand L. (2012) Physiol Rev. 92:1619
  4. Caruana, G. et al. (1999) Oncogene 18:5573
  5. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
  6. Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201.
  7. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
Long Name
Stem Cell Factor Receptor
Entrez Gene IDs
3815 (Human); 16590 (Mouse); 64030 (Rat)
Alternate Names
CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFR; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Loading...

Reviews for Recombinant Human CD117/c-kit Fc Chimera Avi-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human CD117/c-kit Fc Chimera Avi-tag Protein, CF and earn rewards!

Have you used Recombinant Human CD117/c-kit Fc Chimera Avi-tag Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review