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Rat Jagged 1 Antibody

Catalog #: AF599
25 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
AF599
AF599-SP
Differentiation Induced by Jagged 1 and Neutralization by Rat Jagged 1 Antibody.
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Citations (25)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat Jagged 1 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Jagged 1 in ELISAs and Western blots. In sandwich ELISAs, approximately 15% cross-reactivity with recombinant human (rh) Jagged 1 is observed, and less than 0.3% cross-reactivity with rhJagged 2 and recombinant mouse Jagged 2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Jagged 1
Ser32-Asp1068 (Gly57-Arg59 del, Asp63Thr, Arg64Leu, and Val-Arg-Pro-Tyr ins before Lys69)
Accession # Q63722
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat Jagged 1 Fc Chimera (Catalog # 599-JG)
under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
Rat cortical stem cells
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Rat Jagged 1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Rat Jagged 1 Biotinylated Antibody (Catalog # BAF599)

Standard: Recombinant Rat Jagged 1 Fc Chimera Protein, CF (Catalog # 599-JG)

Neutralization
Measured by its ability to neutralize Jagged 1-induced differentiation in the C3H10T1/2 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 3-15 µg/mL in the presence of 3 µg/mL Recombinant Rat Jagged 1 Fc Chimera and 150 ng/mL Recombinant Human BMP‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Differentiation Induced by Jagged 1 and Neutralization by Rat Jagged 1 Antibody. View Larger

Differentiation Induced by Jagged 1 and Neutralization by Rat Jagged 1 Antibody. Recombinant Rat Jagged 1 Fc Chimera (Catalog # 599-JG) induces differentiation in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human BMP-2 (Catalog # 355-BM) in a dose-dependent manner (orange line), as measured by alkaline phosphatase activity. Differentiation elicited by Recombinant Rat Jagged 1 Fc Chimera (3 µg/mL) in the presence of Recombinant Human BMP-2 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF599). The ND50 is typically 3-15 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Jagged 1

Rat Jagged 1 was the first ligand for Notch identified in mammals. Since both the ligands and receptors are transmembrane proteins, direct cell-cell interactions are thought to be required for activating Notch signaling. Jagged 1 is synthesized as a precursor protein that contains a 21 aa signal sequence, a 1048 aa extracellular region, a 25 aa transmembrane (TM) segment and a short, 226 aa cytoplasmic domain. The large extracellular region has a DSL (Delta, Serrate, Lag-2 consensus sequence) domain followed by 16 EGF-like repeats, and a cysteine-rich (CR) region (1). The extracellular region of rJagged 1 binds to multiple Notch receptors on the cell surface as well as in solid phase binding studies. The DSL motif is necessary for binding to Notch receptors and the EGF repeats modulate the affinity of the interaction with Notch receptors (2). Notch signaling is implicated in many developmental processes in a variety of cell types. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation. Some specific areas where Jagged is involved include hematopoiesis, myogenesis, neurogenesis and development of the vasculature (3). For instance soluble, non-transmembrane forms of Jagged 1 influence behavior in fibroblast cells leading to characteristics exhibited by endothelial cells during angiogenesis (4). Soluble Jagged 1 is also capable of maintaining the survival and enhancing the expansion of human stem cells that are capable of reconstituting hematopoietic lineages in vivo (5). Furthermore, Jagged 1 is implicated in human disease: Alagille syndrome, an autosomal dominant disorder characterized by defects in liver, heart, eye, skeletal, craniofacial tissues, and kidney, is caused by mutations in Jagged 1 (6). Depending on cell types and how soluble forms of the ligand are presented, ligand binding can result in activation or inhibition of Notch signaling (7). Rat Jagged 1 shows 98% and 99% aa identity to human and mouse Jagged 1 extracellular domains respectively. Relative to the extracellular region of rat Jagged 2, the aa identity is 58%.

References
  1. Lindsell, C.E. et al. (1995) Cell 80:909.
  2. Shimizu, K. et al. (1999) J. Biol. Chem. 274:32961.
  3. Lewis, J. (1998) Stem Cell & Dev. Biol. 9:583.
  4. Small, D. et al. (2001) J. Biol. Chem. 276:32022.
  5. Karanu, F. et al. (2000) J. Exp. Med. 192:1365.
  6. Joutel, A. and E. Tounier-Lasserve (1998) Stem Cell & Dev. Biol. 9:619.
  7. Hicks, C. et al. (2002) J Neurosci. Res. 68:655.
Entrez Gene IDs
182 (Human); 16449 (Mouse); 29146 (Rat)
Alternate Names
AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1

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Citations for Rat Jagged 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. Human iPSC-derived renal organoids engineered to report oxidative stress can predict drug-induced toxicity
    Authors: Lawrence M, Elhendawi M, Morlock M et al.
    iScience
  2. Stepwise developmental mimicry generates proximal-biased kidney organoids
    Authors: Schnell, J;Miao, Z;Achieng, M;Fausto, CC;Wang, V;Kuyper, F;Thornton, ME;Grubbs, B;Kim, J;Lindström, NO;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Organoid
    Applications: Immunohistochemistry
  3. Measurement of adhesion and traction of cells at high yield (MATCHY) reveals an energetic ratchet driving nephron condensation
    Authors: Liu, J;Prahl, LS;Huang, A;Hughes, AJ;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  4. ?-cell Jagged1 is sufficient but not necessary for islet Notch activity and insulin secretory defects in obese mice
    Authors: Suda, N;Bartolomé, A;Liang, J;Son, J;Yagishita, Y;Siebel, C;Accili, D;Ding, H;Pajvani, UB;
    Molecular metabolism
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. CXCL12 regulates coronary artery dominance in diverse populations and links development to disease
    Authors: Rios Coronado, PE;Zanetti, D;Zhou, J;Naftaly, JA;Prabala, P;Kho, PF;Martínez Jaimes, AM;Hilliard, AT;Pyarajan, S;Dochtermann, D;Million Veteran Program, ;Chang, KM;Winn, VD;Pa?ca, AM;Plomondon, ME;Waldo, SW;Tsao, PS;Clarke, SL;Red-Horse, K;Assimes, TL;
    medRxiv : the preprint server for health sciences
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  6. Bmp9 regulates Notch signaling and the temporal dynamics of angiogenesis via Lunatic Fringe
    Authors: Ristori, T;Thuret, R;Hooker, E;Quicke, P;Lanthier, K;Ntumba, K;Aspalter, IM;Uroz, M;Herbert, SP;Chen, CS;Larrivée, B;Bentley, K;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types:
  7. The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition
    Authors: E Azzoni, V Frontera, G Anselmi, C Rode, C James, EM Deltcheva, AS Demian, J Brown, C Barone, A Patelli, JR Harman, M Nicholls, SJ Conway, E Morrissey, SEW Jacobsen, DB Sparrow, AL Harris, T Enver, MFTR de Bruijn
    Cell Reports, 2021-12-14;37(11):110103.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Hepatocyte TLR4 triggers inter-hepatocyte Jagged1/Notch signaling to determine NASH-induced fibrosis
    Authors: Junjie Yu, Changyu Zhu, Xiaobo Wang, KyeongJin Kim, Alberto Bartolome, Paola Dongiovanni et al.
    Science Translational Medicine
  9. A beta -catenin-driven switch in TCF/LEF transcription factor binding to DNA target sites promotes commitment of mammalian nephron progenitor cells
    Authors: Qiuyu Guo, Albert Kim, Bin Li, Andrew Ransick, Helena Bugacov, Xi Chen et al.
    eLife
  10. BMP9 signaling promotes the normalization of tumor blood vessels
    Authors: C Viallard, C Audiger, N Popovic, N Akla, K Lanthier, I Legault-Na, H Melichar, S Costantino, S Lesage, B Larrivée
    Oncogene, 2020-02-10;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration
    Authors: S Wiszniak, Q Schwarz
    BMC Neurosci, 2019-04-29;20(1):21.
    Species: Mouse
    Sample Types: WholeTissue
    Applications: IHC-F
  12. SMAD4 prevents flow induced arterial-venous malformations by inhibiting Casein Kinase 2
    Authors: Roxana Ola, Sandrine H. Künzel, Feng Zhang, Gael Genet, Raja Chakraborty, Laurence Pibouin-Fragner et al.
    Circulation
  13. Muscle Satellite Cell Cross-Talk with a Vascular Niche Maintains Quiescence via VEGF and Notch Signaling
    Authors: M Verma, Y Asakura, BSR Murakonda, T Pengo, C Latroche, B Chazaud, LK McLoon, A Asakura
    Cell Stem Cell, 2018-10-04;23(4):530-543.e9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Conserved and Divergent Molecular and Anatomic Features of Human and Mouse Nephron Patterning
    Authors: Nils O. Lindström, Tracy Tran, Jinjin Guo, Elisabeth Rutledge, Riana K. Parvez, Matthew E. Thornton et al.
    Journal of the American Society of Nephrology
  15. DACH1 stimulates shear stress-guided endothelial cell migration and coronary artery growth through the CXCL12–CXCR4 signaling axis
    Authors: Andrew H. Chang, Brian C. Raftrey, Gaetano D'Amato, Vinay N. Surya, Aruna Poduri, Heidi I. Chen et al.
    Genes & Development
  16. PI3 kinase inhibition improves vascular malformations in mouse models of hereditary haemorrhagic telangiectasia
    Nat Commun, 2016-11-29;7(0):13650.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Hippo signaling interactions with Wnt/ beta -catenin and Notch signaling repress liver tumorigenesis
    Authors: Wantae Kim, Sanjoy Kumar Khan, Jelena Gvozdenovic-Jeremic, Youngeun Kim, Jason Dahlman, Hanjun Kim et al.
    Journal of Clinical Investigation
  18. Vascular Notch proteins and Notch signaling in the peri-implantation mouse uterus
    Authors: Carrie J. Shawber, Lu Lin, Maria Gnarra, Mark V. Sauer, Virginia E. Papaioannou, Jan K. Kitajewski et al.
    Vascular Cell
  19. Pericytes are progenitors for coronary artery smooth muscle
    Authors: Katharina S Volz, Andrew H Jacobs, Heidi I Chen, Aruna Poduri, Andrew S McKay, Daniel P Riordan et al.
    eLife
  20. Cadherin-based adhesions in the apical endfoot are required for active Notch signaling to control neurogenesis in vertebrates.
    Authors: Hatakeyama J, Wakamatsu Y, Nagafuchi A, Kageyama R, Shigemoto R, Shimamura K
    Development, 2014-04-01;141(8):1671-82.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. OX40L/Jagged1 Cosignaling by GM-CSF–Induced Bone Marrow-Derived Dendritic Cells Is Required for the Expansion of Functional Regulatory T Cells
    Authors: Anupama Gopisetty, Palash Bhattacharya, Christine Haddad, Joseph C. Bruno, Chenthamarakshan Vasu, Lucio Miele et al.
    The Journal of Immunology
  22. ALK1 Signaling Inhibits Angiogenesis by Cooperating with the Notch Pathway
    Authors: Bruno Larrivée, Claudia Prahst, Emma Gordon, Raquel del Toro, Thomas Mathivet, Antonio Duarte et al.
    Developmental Cell
  23. Roles of Pofut1 and O-fucose in mammalian Notch signaling.
    Authors: Stahl M, Uemura K, Ge C, Shi S, Tashima Y, Stanley P
    J. Biol. Chem., 2008-03-17;283(20):13638-51.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Intraoperative delivery of the Notch ligand Jagged-1 regenerates appendicular and craniofacial bone defects
    Authors: DW Youngstrom, R Senos, RL Zondervan, JD Brodeur, AR Lints, DR Young, TL Mitchell, ME Moore, MH Myers, WJ Tseng, KM Loomes, KD Hankenson
    NPJ Regen Med, 2017-12-15;2(0):32.
  25. Single-Cell Profiling Reveals Sex, Lineage, and Regional Diversity in the Mouse Kidney.
    Authors: Ransick, A, LindstrOm, N O Et al.
    Dev Cell

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Rat Jagged 1 Antibody
By Tracy Tran on 04/22/2016
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Embryonic kidney Species: Mouse
Immunocytochemistry/Immunofluorescence Rat Jagged 1 Antibody AF599