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Recombinant Rat Jagged 1 Fc Chimera Protein, CF

Catalog #: 599-JG
28 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
599-JG-100
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Citations (28)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Rat Jagged 1 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 0.5-2.0 µg/mL.
Source
Mouse myeloma cell line, NS0-derived rat Jagged 1 protein
Rat Jagged 1
Ser32-Asp1067
with substitutions GGARN56-60AE and DR63-64TLVRPY
Accession # Q63722.2		 IEGRMD Human IgG1
(Pro100-Lys330) 		6-His tag
N-terminus C-terminus
Accession #
Q63722.2
N-terminal Sequence
Analysis
Ser32
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
141.7 kDa (monomer)
SDS-PAGE
180 kDa, reducing conditions

Product Datasheets

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599-JG

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

599-JG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Jagged 1

Rat Jagged1 was the first ligand for Notch identified in mammals. Since both the ligands and receptors are transmembrane proteins, direct cell-cell interactions are thought to be required for activating Notch signaling. Jagged1 is synthesized as a precursor protein that contains a 21 aa signal sequence, a 1048 aa extracellular region, a 25 aa transmembrane (TM) segment and a short, 226 aa cytoplasmic domain. The large extracellular region has a DSL (Delta, Serrate, Lag-2 consensus sequence) domain followed by 16 EGF-like repeats, and a cysteine-rich (CR) region (1). The extracellular region of rJagged1 binds to multiple Notch receptors on the cell surface as well as in solid phase binding studies. The DSL motif is necessary for binding to Notch receptors and the EGF repeats modulate the affinity of the interaction with Notch receptors (2). Notch signaling is implicated in many developmental processes in a variety of cell types. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation. Some specific areas where Jagged is involved include hematopoiesis, myogenesis, neurogenesis and development of the vasculature (3). For instance soluble, non-transmembrane forms of Jagged1 influence behavior in fibroblast cells leading to characteristics exhibited by endothelial cells during angiogenesis (4). Soluble Jagged1 is also capable of maintaining the survival and enhancing the expansion of human stem cells that are capable of reconstituting hematopoietic lineages in vivo (5). Furthermore, Jagged1 is implicated in human disease: Alagille syndrome, an autosomal dominant disorder characterized by defects in liver, heart, eye, skeletal, craniofacial tissues, and kidney, is caused by mutations in Jagged1 (6). Depending on cell types and how soluble forms of the ligand are presented, ligand binding can result in activation or inhibition of Notch signaling (7). Rat Jagged1 shows 98% and 99% aa identity to human and mouse Jagged1 extracellular domains respectively. Relative to the extracellular region of rat Jagged2, the aa identity is 58%.

References
  1. Lindsell, C.E. et al. (1995) Cell 80:909.
  2. Shimizu, K. et al. (1999) J. Biol. Chem. 274:32961.
  3. Lewis, J. (1998) Stem Cell & Dev. Biol. 9:583.
  4. Small, D. et al. (2001) J. Biol. Chem. 276:32022.
  5. Karanu, F. et al. (2000) J. Exp. Med. 192:1365.
  6. Joutel, A. and E. Tounier-Lasserve (1998) Stem Cell & Dev. Biol. 9:619.
  7. Hicks, C. et al. (2002) J Neurosci. Res. 68:655.
Entrez Gene IDs
182 (Human); 16449 (Mouse); 29146 (Rat)
Alternate Names
AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1

Citations for Recombinant Rat Jagged 1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. Cis inhibition of NOTCH1 through JAGGED1 sustains embryonic hematopoietic stem cell fate
    Authors: Thambyrajah, R;Maqueda, M;Neo, WH;Imbach, K;Guillén, Y;Grases, D;Fadlullah, Z;Gambera, S;Matteini, F;Wang, X;Calero-Nieto, FJ;Esteller, M;Florian, MC;Porta, E;Benedito, R;Göttgens, B;Lacaud, G;Espinosa, L;Bigas, A;
    Nature communications
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. YY1/miR-140-5p/Jagged1/Notch axis mediates cartilage progenitor/stem cells fate reprogramming in knee osteoarthritis
    Authors: Chen, Y;Liao, G;Ma, T;Li, L;Yang, J;Shen, B;Lu, Y;Si, H;
    International immunopharmacology
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  3. CCN1 interacts with integrins to regulate intestinal stem cell proliferation and differentiation
    Authors: JH Won, JS Choi, JI Jun
    Nature Communications, 2022-06-03;13(1):3117.
    Species: Mouse
    Sample Types: Organoids
    Applications: Bioassay
  4. Notch1 activation of Jagged1 contributes to differentiation of mesenchymal stem cells into endothelial cells under cigarette smoke extract exposure
    Authors: Y Cheng, W Gu, G Zhang, X Guo
    BMC pulmonary medicine, 2022-04-11;22(1):139.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  5. GIT1 protects against breast cancer growth through negative regulation of Notch
    Authors: S Zhang, A Miyakawa, M Wickström, C Dyberg, L Louhivuori, M Varas-Godo, K Kemppainen, S Kanatani, D Kaczynska, ID Ellström, L Elfman, P Kronqvist, H Repo, K Mikoshiba, C Sahlgren, JI Johnsen, P Uhlén
    Nature Communications, 2022-03-22;13(1):1537.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Tumor-derived Jagged1 promotes cancer progression through immune evasion
    Authors: J Meng, YZ Jiang, S Zhao, Y Tao, T Zhang, X Wang, Y Zhang, K Sun, M Yuan, J Chen, Y Wei, X Lan, M Chen, CJ David, Z Chang, X Guo, D Pan, M Chen, ZM Shao, Y Kang, H Zheng
    Cell Reports, 2022-03-08;38(10):110492.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  7. Inhibition of Notch Signaling Promotes the Differentiation of Epicardial Progenitor Cells into Adipocytes
    Authors: B Liu, D Wang, T Xiong, Y Liu, X Jing, J Du, Q She
    Stem Cells International, 2021-04-09;2021(0):8859071.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Role of Jagged1-mediated Notch Signaling Activation in the Differentiation and Stratification of the Human Limbal Epithelium
    Authors: S González, M Halabi, D Ju, M Tsai, SX Deng
    Cells, 2020-08-22;9(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2
    Authors: S Kakuda, RK Lopilato, A Ito, RS Haltiwange
    J. Biol. Chem., 2020-08-19;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Downregulation of Notch Signaling in Kras-Induced Gastric Metaplasia
    Authors: WC Chung, Y Zhou, A Atfi, K Xu
    Neoplasia, 2019-07-02;21(8):810-821.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Inhibition of the Notch1 Pathway Promotes the Effects of Nucleus Pulposus Cell-Derived Exosomes on the Differentiation of Mesenchymal Stem Cells into Nucleus Pulposus-Like Cells in Rats
    Authors: WR Lan, S Pan, HY Li, C Sun, X Chang, K Lu, CQ Jiang, R Zuo, Y Zhou, CQ Li
    Stem Cells Int, 2019-05-06;2019(0):8404168.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  12. Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling
    Authors: KB Kaylan, IC Berg, MJ Biehl, A Brougham-C, I Jain, SM Jamil, LH Sargeant, NJ Cornell, LT Raetzman, GH Underhill
    Elife, 2018-12-27;7(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Aberrant activation of Notch-1 signaling inhibits podocyte restoration after islet transplantation in a rat model of diabetic nephropathy
    Authors: Y He, M Zhang, Y Wu, H Jiang, H Fu, Y Cai, Z Xu, C Liu, B Chen, T Yang
    Cell Death Dis, 2018-09-20;9(10):950.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Characterization of smooth muscle cells from human atherosclerotic lesions and their responses to Notch signaling
    Authors: J Davis-Know, JE Turner, A Turner, S Damian-Lor, N Hagler, T Henderson, IF Emery, K Bond, CW Duarte, CPH Vary, J Eldrup-Jor, L Liaw
    Lab. Invest., 2018-05-23;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Notch Ligand Binding Assay Using Flow Cytometry
    Authors: S Varshney, P Stanley
    Bio Protoc, 2017-12-05;7(23):.
    Species: Human, Rat
    Sample Types: Recombinant Protein, Whole Cells
    Applications: Bioassay
  16. Selective regulation of Notch ligands during angiogenesis is mediated by vimentin
    Authors: D Antfolk, M Sjöqvist, F Cheng, K Isoniemi, CL Duran, A Rivero-Mul, C Antila, R Niemi, S Landor, CVC Bouten, KJ Bayless, JE Eriksson, CM Sahlgren
    Proc. Natl. Acad. Sci. U.S.A., 2017-05-22;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  17. Jagged1/Notch3 Signaling Modulates Hemangioma-Derived Pericyte Proliferation and Maturation
    Authors: Yi Ji
    Cell. Physiol. Biochem, 2016-12-07;40(5):895-907.
    Species: Rat
    Sample Types: Protein
    Applications: Bioassay
  18. MicroRNA-199b Modulates Vascular Cell Fate During iPS Cell Differentiation by Targeting the Notch Ligand Jagged1 and Enhancing VEGF Signaling.
    Authors: Chen T, Margariti A, Kelaini S, Cochrane A, Guha S, Hu Y, Stitt A, Zhang L, Xu Q
    Stem Cells, 2015-05-01;33(5):1405-18.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. CCN1 induces hepatic ductular reaction through integrin alphavbeta(5)-mediated activation of NF-kappaB.
    Authors: Kim K, Chen C, Alpini G, Lau L
    J Clin Invest, 2015-03-30;125(5):1886-900.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  20. beta-site amyloid precursor protein cleaving enzyme 1(BACE1) regulates Notch signaling by controlling the cleavage of Jagged 1 (Jag1) and Jagged 2 (Jag2) proteins.
    Authors: He W, Hu J, Xia Y, Yan R
    J Biol Chem, 2014-07-25;289(30):20630-7.
    Species: Rat
    Sample Types: Protein
    Applications: Enzyme Assay Substrate
  21. PKCzeta regulates Notch receptor routing and activity in a Notch signaling-dependent manner.
    Authors: Sjoqvist M, Antfolk D, Ferraris S, Rraklli V, Haga C, Antila C, Mutvei A, Imanishi S, Holmberg J, Jin S, Eriksson J, Lendahl U, Sahlgren C
    Cell Res, 2014-03-25;24(4):433-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. A receptor-specific function for Notch2 in mediating vascular smooth muscle cell growth arrest through cyclin-dependent kinase inhibitor 1B.
    Authors: Boucher, Joshua M, Harrington, Anne, Rostama, Bahman, Lindner, Volkhard, Liaw, Lucy
    Circ Res, 2013-08-21;113(8):975-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Notch1 signaling modulates neuronal progenitor activity in the subventricular zone in response to aging and focal ischemia.
    Authors: Sun F, Mao X, Xie L, Ding M, Shao B, Jin K
    Aging Cell, 2013-08-06;12(6):978-87.
    Species: Rat
    Sample Types: In Vivo
    Applications: Bioassay
  24. Protective astrogenesis from the SVZ niche after injury is controlled by Notch modulator Thbs4.
    Authors: Benner, Eric J, Luciano, Dominic, Jo, Rebecca, Abdi, Khadar, Paez-Gonzalez, Patricia, Sheng, Huaxin, Warner, David S, Liu, Chunlei, Eroglu, Cagla, Kuo, Chay T
    Nature, 2013-04-24;497(7449):369-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Attenuation of Notch signaling promotes the differentiation of neural progenitors into neurons in the hippocampal CA1 region after ischemic injury.
    Authors: Oya S, Yoshikawa G, Takai K, Tanaka JI, Higashiyama S, Saito N, Kirino T, Kawahara N
    Neuroscience, 2008-10-31;158(2):683-92.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  26. Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage.
    Authors: Elkabetz Y, Panagiotakos G, Al Shamy G, Socci ND, Tabar V, Studer L
    Genes Dev., 2008-01-15;22(2):152-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Notch1 engagement by Delta-like-1 promotes differentiation of B lymphocytes to antibody-secreting cells.
    Authors: Santos MA, Sarmento LM, Rebelo M, Doce AA, Maillard I, Dumortier A, Neves H, Radtke F, Pear WS, Parreira L, Demengeot J
    Proc. Natl. Acad. Sci. U.S.A., 2007-09-18;104(39):15454-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Differentiation of ES cells into cerebellar neurons.
    Authors: Salero E, Hatten ME
    Proc. Natl. Acad. Sci. U.S.A., 2007-02-09;104(8):2997-3002.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Rat Jagged 1 Fc Chimera Protein, CF
By Anonymous on 08/25/2021
Application: Stem/Immune cell maintenance or differentiation
Stem/Immune cell maintenance or differentiation Recombinant Rat Jagged 1 Fc Chimera Protein, CF 599-JG