Home / CXCL2/GRO beta/MIP-2/CINC-3 / Rat CXCL2/GRO beta /MIP-2/CINC-3 Antibody

Rat CXCL2/GRO beta /MIP-2/CINC-3 Antibody

Catalog #: MAB525
4 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB525
MAB525-SP
Chemotaxis Induced by CXCL2/CINC‑3 and Neutral-ization by Rat CXCL2/ CINC‑3 Antibody.
1 Image
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat CXCL2/GRO beta /MIP-2/CINC-3 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat CXCL2/GRO beta /MIP‑2/CINC‑3 in ELISAs and Western blots. In sandwich ELISAs, less than 0.5% cross-reactivity with recombinant rat (rr) CXCL3 and less than 0.1% cross-reactivity with rrCXCL1, rrCINC-2 alpha, recombinant mouse (rm) CXCL9 or rmCXCL2 is observed. In Western blots, less than 3% cross-reactivity with recombinant human (rh) CXCL8, recombinant porcine CXCL8, or rrCINC-2 alpha and no cross‑reactivity with rrCXCL1, 3, rmCXCL1, 2, 6, 9, 10, 12, rhCXLC1, 2, 3, 5, 6, 7, 9,10, 11, 12 or 13 is observed.
Source
Monoclonal Mouse IgG2B Clone # 123802
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant rat CXCL2/GRO beta /MIP‑2/CINC‑3
Ser32-Asn100
Accession # P30348
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Rat CXCL2/GRO beta /MIP‑2/CINC‑3 (Catalog # 525-C3)

Rat CXCL2/CINC-3 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Rat CXCL2/GRO beta /MIP‑2/CINC‑3 Biotinylated Antibody (Catalog # BAF525)

Standard: Recombinant Rat CXCL2/CINC-3 Protein (Catalog # 525-C3)

Neutralization
Measured by its ability to neutralize CXCL2/GRO beta /MIP‑2/CINC‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.4-2.0 µg/mL in the presence of 0.1 µg/mL Recombinant Rat CXCL2/GRO beta /MIP‑2/CINC‑3.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CXCL2/CINC‑3 and Neutral-ization by Rat CXCL2/ CINC‑3 Antibody. View Larger

Chemotaxis Induced by CXCL2/CINC‑3 and Neutral-ization by Rat CXCL2/ CINC‑3 Antibody. Recombinant Rat CXCL2/CINC-3 (Catalog # 525-C3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL2/ CINC-3 (0.1 µg/mL) is neutral-ized (green line) by increasing concentrations of Mouse Anti-Rat CXCL2/CINC-3 Monoclonal Antibody (Catalog # MAB525). The ND50 is typically 0.4-2.0 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL2/GRO beta/MIP-2/CINC-3

Cytokine-induced neutrophil chemoattractant 3 (CINC-3) has been found to be expressed by cytokine-stimulated rat alveolar macrophages and fibroblasts. Based on its protein and DNA sequences, CINC-3 (also known as rat MIP-2, GRO beta and CXCL2) is a member of the alpha (CXC) subfamily of chemokines.

CINC-3 cDNA encodes a 100 amino acid (aa) residue precursor protein with a 31 aa signal peptide that is cleaved to yield a 69 aa mature secreted protein. The protein sequence of rat CINC-3 shares approximately 88% identity with murine MIP-2. Characteristic of ELR containing CXC chemokines, CINC-3 is known to be a potent chemotactic factor for rat neutrophils in vitro and in vivo. On the basis of cross-desensitization results of the various CINC proteins, it has been postulated that rat neutrophils have at least two classes of CINC receptors: a class of CINC-3 specific receptors as well as a second common receptor shared by all CINCs.

References
  1. Nakagawa, H. et al. (1994) Biochem. J. 301:545.
  2. Huang, S. et al. (1992) Am. J. Pathol. 141:981.
Entrez Gene IDs
2920 (Human); 20310 (Mouse); 114105 (Rat)
Alternate Names
chemokine (C-X-C motif) ligand 2; CINC-2a; CINC3; CINC-3; C-X-C Motif Chemokine 2; CXCL2; GRO beta; GRO2 oncogene; GRO2; GROB; Gro-beta; Growth-regulated protein beta; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MGSA-beta; MIP2; MIP-2; MIP2A; MIP-2a; MIP2-alpha; SCYB2

Product Datasheets

You must select a language.

x
Product Datasheet

Citations for Rat CXCL2/GRO beta /MIP-2/CINC-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

  1. Pharmacological Blockade of Spinal CXCL3/CXCR2 Signaling by NVP CXCR2 20, a Selective CXCR2 Antagonist, Reduces Neuropathic Pain Following Peripheral Nerve Injury
    Authors: Piotrowska A, Rojewska E, Pawlik K et al.
    Front Immunol
  2. Development and comparison of a minimally-invasive model of autologous clot pulmonary embolism in Sprague-Dawley and Copenhagen rats
    Authors: Michael S Runyon, Michael A Gellar, Nina Sanapareddy, Jeffrey A Kline, John A Watts
    Thrombosis Journal
  3. Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines.
    Authors: Miura TA, Wang J, Holmes KV, Mason RJ
    Virology, 2007-09-05;369(2):288-98.
    Species: Rat
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  4. Cardiac inflammation contributes to right ventricular dysfunction following experimental pulmonary embolism in rats.
    Authors: Watts JA, Zagorski J, Gellar MA, Stevinson BG, Kline JA
    J. Mol. Cell. Cardiol., 2006-06-30;41(2):296-307.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: ELISA Development

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Rat CXCL2/GRO beta /MIP-2/CINC-3 Antibody

There are currently no reviews for this product. Be the first to review Rat CXCL2/GRO beta /MIP-2/CINC-3 Antibody and earn rewards!

Have you used Rat CXCL2/GRO beta /MIP-2/CINC-3 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review