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Rat CXCL2/GRO beta /MIP-2/CINC-3 Biotinylated Antibody

Catalog #: BAF525
3 Citations Datasheet
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BAF525
Product Details
Citations (3)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat CXCL2/GRO beta /MIP-2/CINC-3 Biotinylated Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat CXCL2/GRO beta /MIP‑2/CINC‑3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant rat (rr) CINC‑2 beta  is observed and less than 0.1% cross-reactivity with recombinant mouse (rm) MIG, rmMIP-2, rrCINC-1, and rrCINC-2 alpha  is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat CXCL2/GRO beta /MIP‑2/CINC‑3
Ser32-Asn100
Accession # P30348
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat CXCL2/GRO beta /MIP‑2/CINC‑3 (Catalog # 525-C3)

Rat CXCL2/CINC-3 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Rat CXCL2/GRO beta /MIP‑2/CINC‑3 Antibody (Catalog # MAB525)

Standard: Recombinant Rat CXCL2/CINC-3 Protein (Catalog # 525-C3)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL2/GRO beta/MIP-2/CINC-3

Cytokine-induced neutrophil chemoattractant 3 (CINC-3) has been found to be expressed by cytokine-stimulated rat alveolar macrophages and fibroblasts. Based on its protein and DNA sequences, CINC-3 (also known as rat MIP-2, GRO beta and CXCL2) is a member of the alpha (CXC) subfamily of chemokines.

CINC-3 cDNA encodes a 100 amino acid (aa) residue precursor protein with a 31 aa signal peptide that is cleaved to yield a 69 aa mature secreted protein. The protein sequence of rat CINC-3 shares approximately 88% identity with murine MIP-2. Characteristic of ELR containing CXC chemokines, CINC-3 is known to be a potent chemotactic factor for rat neutrophils in vitro and in vivo. On the basis of cross-desensitization results of the various CINC proteins, it has been postulated that rat neutrophils have at least two classes of CINC receptors: a class of CINC-3 specific receptors as well as a second common receptor shared by all CINCs.

References
  1. Nakagawa, H. et al. (1994) Biochem. J. 301:545.
  2. Huang, S. et al. (1992) Am. J. Pathol. 141:981.
Entrez Gene IDs
2920 (Human); 20310 (Mouse); 114105 (Rat)
Alternate Names
chemokine (C-X-C motif) ligand 2; CINC-2a; CINC3; CINC-3; C-X-C Motif Chemokine 2; CXCL2; GRO beta; GRO2 oncogene; GRO2; GROB; Gro-beta; Growth-regulated protein beta; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MGSA-beta; MIP2; MIP-2; MIP2A; MIP-2a; MIP2-alpha; SCYB2

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Citations for Rat CXCL2/GRO beta /MIP-2/CINC-3 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Development and comparison of a minimally-invasive model of autologous clot pulmonary embolism in Sprague-Dawley and Copenhagen rats
    Authors: Michael S Runyon, Michael A Gellar, Nina Sanapareddy, Jeffrey A Kline, John A Watts
    Thrombosis Journal
  2. Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines.
    Authors: Miura TA, Wang J, Holmes KV, Mason RJ
    Virology, 2007-09-05;369(2):288-98.
    Species: Rat
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  3. Cardiac inflammation contributes to right ventricular dysfunction following experimental pulmonary embolism in rats.
    Authors: Watts JA, Zagorski J, Gellar MA, Stevinson BG, Kline JA
    J. Mol. Cell. Cardiol., 2006-06-30;41(2):296-307.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: ELISA Development

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