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Proteome Profiler Human XL Oncology Array

Catalog #: ARY026
28 Citations 7 Reviews Datasheet
Contains 4 membranes-each spotted in duplicate with 84 different cancer-related antibodies
Catalog # Availability Size / Price Qty
ARY026
R&D Systems Proteome Profiler Antibody Array Kits
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Product Details
Procedure
Citations (28)
FAQs
Supplemental Products
Reviews (7)
Summary Product Datasheets Data Images

Proteome Profiler Human XL Oncology Array Summary

Kit Summary

To detect differences in 84 cancer-related proteins between samples. No specialized equipment is necessary.

*If using a LI-COR, additional reagents and protocol modifications are required.  Refer to protocol.

 

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell lysates, tissue lysates, serum, plasma, urine, saliva, or human milk are diluted and incubated with the Human XL Oncology Array overnight. The array is washed to remove unbound proteins, followed by incubation with a cocktail of biotinylated detection antibodies. Streptavidin-HRP and chemiluminescent detection reagents are applied, and a signal is produced at each capture spot corresponding to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.

 

Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Reagents are stable for 12 months from date of receipt when stored in the dark at 2° C to 8°.

 

Simultaneously detect the levels of these cancer-related proteins in a single sample.
AFP ErbB4 MMP-2
Amphiregulin FGF basic MMP-3
Angiopoietin-1 FoxC2  MMP-9
ANGPTL4 FKHR MSP/MST1
ENPP-2/Autotaxin Galectin-3 MUC-1
AXL GM-CSF Nectin-4
BCL-X HCG Osteopontin
CA125/MUC-16 HGF R/c-Met p27/Kip1
E-Cadherin HIF-1alpha p53
VE-Cadherin HNF-3beta PDGF-AA
CAP-G HO-1/HMOX1 CD31/PECAM-1
CA-9 ICAM-1/CD54 Progesterone R
Cathepsin B CD25/IL-2 R alpha Progranulin
Cathepsin D IL-6 Prolactin
Cathepsin S CXCL8/IL-8 Prostasin
CEACAM-5 IL-18 Bpa E-Selectin
Decorin KLK-3/PSA Maspin
DKK-1 KLK-5 PAI-1/Serpin E1
DLL-1 KLK-6 SNAIL
EGF R/ErbB1 Leptin (OB) SPARC
Endoglin/CD105 Lumican Survivin
Endostatin CCL2/MCP-1 Tenascin-C
Enolase 2 CCL8/MCP-2 THBS-1
eNOS CCL7/MCP-3 TIE-2
EpCAM M-CSF UPA-1
ER-alpha Mesothelin VCAM-1
ErbB2 CCL3/MIP-1alpha VEGF
ErbB3 CCL20/MIP-3alpha Vimentin

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Human

Product Datasheets

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Product Datasheet

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, relative expression levels of human cancer-related proteins in samples can be determined using the following procedure:

  • Prepare membrane and incubate with prepared sample
  • Incubate the membrane with Detection Antibody Cocktail
  • Incubate the membrane array with Streptavidin-HRP
  • Develop the membrane array with Chemi Reagents 1 and 2
  • Expose the membrane array to autoradiography film

ARY026 Protocol Graphic Summary
View Printable Protocol

 
Kit Contents
  • Rectangular 4-Well Multi-dish
  • 4 Human XL Oncology Array nitrocellulose membranes spotted with 84 different antibodies to cancer-related proteins
  • Array Buffer 4
  • Array Buffer 6
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Human XL Oncology Array
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

 

Other Supplies Required

Reagents

  • Pipettes and pipette tips
  • Gloves
  • Plastic container with the capacity to hold 50 mL (for washing the arrays)
  • Plastic transparent sheet protector (trimmed to 10 cm x 12 cm and open on three sides)
  • Plastic wrap
  • Absorbent lab wipes (KimWipes® or equivalent)
  • Paper towels
  • X-ray film (Kodak BioMax™ Light-1) or equivalent
  • Flat-tipped tweezers

Equipment

  • Rocking platform shaker
  • Microcentrifuge
  • Autoradiography cassette
  • Film developer
  • Flatbed scanner with transparency adapter capable of transmission mode
  • Computer capable of running image analysis software and Microsoft Excel

Other Supplies Required for Cell Lysate Samples

  • Phosphate-Buffered Saline (PBS)
  • Lysis buffer 17
  • Aprotinin
  • Leupeptin (Catalog # EI002)
  • Pepstatin (Catalog # EI003)

Other Supplies Required for Tissue Lysate Samples

  • Protease Inhibitor Cocktail
  • Igepal® CA-630
  • Sodium deoxycholate
  • Sodium dodecyl sulfate

 

Procedure Overview

R&D Systems Protocol for Multiple Analyte Detection Using the Proteome Profiler™ Human XL Oncology Array Kit, Panel A (Catalog # ARY026)

Add 2 mL of Array Buffer 6 to each well of the supplied 4-Well Multi-dish.

ARY026 Step 1

Place each array membrane in a separate well of the 4-Well Multi-dish

Incubate for one hour on a rocking platform shaker.

ARY026 Step 2

Add 0.5 mL Array Buffer 4 to each sample.

Adjust volume of each sample to final volume of 1.5 mL with Array Buffer 6.

ARY026 Step 3

Replace the Array Buffer 6 in each well of the 4-Well Multi-dish with prepared samples.

Incubate overnight at 2 °C to 8 °C on a rocking platform.

Wash each array membrane 3 times with 1X Wash Buffer in a separate container.

Wash each well of the 4-Well Multi-dish with 1X Wash Buffer.

ARY026 Step 4

Add 30 µL of Detection Antibody Cocktail to 1.5 mL of Array Buffer 4/6 for each array.

ARY026 Step 5

Pipette 1.5 mL diluted Detection Antibody Cocktail into each well of the 4-Well Multi-dish.

ARY026 Step 6

Return each array membrane to the 4-Well Multi-dish containing diluted Detection Antibody Cocktail.

Incubate for one hour on a rocking platform shaker.

Wash each array membrane 3 times with 1X Wash Buffer in a separate container.

Wash each well of the 4-Well Multi-dish with 1X Wash Buffer.

ARY026 Step 7

Add 2 mL of diluted Streptavidin-HRP to each well of the 4-Well Multi-dish.

Place the array membrane in the diluted Streptavidin-HRP solution.

Incubate for 30 minutes on a rocking platform shaker.

Wash each array membrane 3 times with 1X Wash Buffer in a separate container.

ARY026 Step 8

Place the array membrane on a plastic sheet protector.

Pipette 1 mL of the prepared Chemi Reagent Mix evenly onto the membrane.

Cover the membrane with the top sheet of the plastic protector

Incubate for 1 minute.

Blot off excess Chemi Reagent Mix.

Wrap the membrane and sheet protector in plastic wrap.

Place the wrapped array membrane in an autoradiography film cassette and expose to X-ray film.

ARY026 Step 9

Citations for Proteome Profiler Human XL Oncology Array

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. Gastric Inhibitory Polypeptide Receptor (GIPR) Overexpression Reduces the Tumorigenic Potential of Retinoblastoma Cells
    Authors: Haase, A;Alefeld, E;Yalinci, F;Meenen, DV;Busch, MA;Dünker, N;
    Cancers  2024-04-25
  2. BAP31 Promotes Angiogenesis via Galectin-3 Upregulation in Neuroblastoma
    Authors: Namusamba, M;Wu, Y;Yang, J;Zhang, Q;Wang, C;Wang, T;Wang, B;
    International journal of molecular sciences  2024-03-03
  3. Contributions of viral oncogenes of HPV-18 and hypoxia to oxidative stress and genetic damage in human keratinocytes
    Authors: Hochmann, J;Millán, M;Hernández, P;Lafon-Hughes, L;Aiuto, N;Silva, A;Llaguno, J;Alonso, J;Fernández, A;Pereira-Prado, V;Sotelo-Silveira, J;Bologna-Molina, R;Arocena, M;
    Scientific reports  2023-10-18
  4. Mutated HRAS activates YAP1-AXL signaling to drive metastasis of head and neck cancer
    Authors: S Jagadeesha, M Prasad, M Badarni, T Ben Lulu, VB Liju, S Mathukkada, C Saunders, AB Shnerb, J Zorea, KM Yegodayev, M Wainer, L Vtorov, I Allon, O Cohen, G Gausdal, D Friedmann-, SC Cheong, AL Ho, AJ Rosenberg, L Kessler, F Burrows, D Kong, JR Grandis, JS Gutkind, M Elkabets
    Cancer Research, 2023-04-04;0(0):.  2023-04-04
  5. A serum protein signature at the time of Uveal Melanoma diagnosis predicts long-term patient survival
    Authors: C Herrspiege, F Plastino, E Lardner, S Seregard, PA Williams, H André, G Stålhammar
    BMC Cancer, 2023-03-27;23(1):277.  2023-03-27
  6. gp120 Envelope Glycoproteins of HIV-1 Group M Subtype A and Subtype B Differentially Affect Gene Expression in Human Vascular Endothelial Cells
    Authors: AJ Suh, DI Suzuki, SG Gychka, TI Brelidze, YJ Suzuki
    International Journal of Molecular Sciences, 2023-02-10;24(4):.  2023-02-10
  7. Extracellular Vesicles Released from Cancer Cells Promote Tumorigenesis by Inducing Epithelial to Mesenchymal Transition via beta-Catenin Signaling
    Authors: V Malyla, KR Paudel, G Rubis, NG Hansbro, PM Hansbro, K Dua
    International Journal of Molecular Sciences, 2023-02-09;24(4):.  2023-02-09
  8. gp120 envelope glycoproteins of HIV-1 Group M Subtype A and Subtype B differentially affect gene expression in human vascular endothelial cells
    Authors: AJ Suh, DI Suzuki, SG Gychka, TI Brelidze, YJ Suzuki
    bioRxiv : the preprint server for biology, 2023-01-03;0(0):.  2023-01-03
  9. Melanoma Mediated Disruption of Brain Endothelial Barrier Integrity Is Not Prevented by the Inhibition of Matrix Metalloproteinases and Proteases
    Authors: A Anchan, G Finlay, CE Angel, JJW Hucklesby, SE Graham
    Biosensors, 2022-08-19;12(8):.  2022-08-19
  10. CARD14 Signalling Ensures Cell Survival and Cancer Associated Gene Expression in Prostate Cancer Cells
    Authors: D Vanneste, J Staal, M Haegman, Y Driege, M Carels, E Van Nuffel, P De Bleser, Y Saeys, R Beyaert, IS Afonina
    Biomedicines, 2022-08-18;10(8):.  2022-08-18
  11. A Hybrid Epithelial to Mesenchymal Transition in Ex Vivo Cutaneous Squamous Cell Carcinoma Tissues
    Authors: CS Pulford, CK Uppalapati, MR Montgomery, RL Averitte, EE Hull, KJ Leyva
    International Journal of Molecular Sciences, 2022-08-16;23(16):.  2022-08-16
  12. Small molecule-based detection of non-canonical RNA G-quadruplex structures that modulate protein translation
    Authors: Y Katsuda, SI Sato, M Inoue, H Tsugawa, T Kamura, T Kida, R Matsumoto, S Asamitsu, N Shioda, S Shiroto, Y Oosawatsu, K Yatsuzuka, Y Kitamura, M Hagihara, T Ihara, M Uesugi
    Nucleic Acids Research, 2022-08-12;0(0):.  2022-08-12
  13. Evaluation of the Cytotoxic Activity and Anti-Migratory Effect of Berberine-Phytantriol Liquid Crystalline Nanoparticle Formulation on Non-Small-Cell Lung Cancer In Vitro
    Authors: AM Alnuqaydan, AG Almutary, M Azam, B Manandhar, GHS Yin, LL Yen, T Madheswara, KR Paudel, PM Hansbro, DK Chellappan, K Dua
    Pharmaceutics, 2022-05-24;14(6):.  2022-05-24
  14. Nuclear Localization of BRAFV600E Is Associated with HMOX-1 Upregulation and Aggressive Behavior of Melanoma Cells
    Authors: M Zerfaoui, E Toraih, E Ruiz, Y Errami, AS Attia, M Krzysztof, ZY Abd Elmage, E Kandil
    Cancers, 2022-01-09;14(2):.  2022-01-09
  15. Transcriptomic identification of HBx-associated hub genes in hepatocellular carcinoma
    Authors: Z Ni, J Lu, W Huang, H Khan, X Wu, D Huang, G Shi, Y Niu, H Huang
    PeerJ, 2021-12-22;9(0):e12697.  2021-12-22
  16. The EGF Domains of MUC4 Oncomucin Mediate HER2 Binding Affinity and Promote Pancreatic Cancer Cell Tumorigenesis
    Authors: N Stoup, M Liberelle, C Schulz, S Cavdarli, R Vasseur, R Magnez, F Lahdaoui, N Skrypek, F Peretti, F Frénois, X Thuru, P Melnyk, N Renault, N Jonckheere, N Lebègue, I Van Seunin
    Cancers, 2021-11-16;13(22):.  2021-11-16
  17. MASTL regulates EGFR signaling to impact pancreatic cancer progression
    Authors: I Fatima, S Barman, J Uppada, S Chauhan, S Rauth, S Rachagani, MP Ponnusamy, L Smith, G Talmon, AB Singh, SK Batra, P Dhawan
    Oncogene, 2021-07-30;0(0):.  2021-07-30
  18. Hypoxia-induced release, nuclear translocation, and signaling activity of a DLK1 intracellular fragment in glioma
    Authors: ES Grassi, V Pantazopou, A Pietras
    Oncogene, 2020-03-24;0(0):.  2020-03-24
  19. Immuno-imaging of ICAM-1 in tumours by SPECT
    Authors: M Mosley, J Baguña Tor, D Allen, B Cornelisse
    Nucl. Med. Biol., 2020-02-25;84(0):73-79.  2020-02-25
  20. New Paradigms to Assess Consequences of Long-Term, Low-Dose Curcumin Exposure in Lung Cancer Cells
    Authors: G Smagurausk, J Mahale, K Brown, AL Thomas, LM Howells
    Molecules, 2020-01-16;25(2):.  2020-01-16
  21. Gene Expression Comparison between the Lymph Node-Positive and -Negative Reveals a Peculiar Immune Microenvironment Signature and a Theranostic Role for WNT Targeting in Pancreatic Ductal Adenocarcinoma: A Pilot Study
    Authors: A Argentiero, S De Summa, R Di Fonte, RM Iacobazzi, L Porcelli, M Da Vià, O Brunetti, A Azzariti, N Silvestris, AG Solimando
    Cancers (Basel), 2019-07-04;11(7):.  2019-07-04
  22. Preclinical evaluation of an unconventional ruthenium-gold-based chemotherapeutic: RANCE-1, in clear cell renal cell carcinoma
    Authors: BT Elie, K Hubbard, Y Pechenyy, B Layek, S Prabha, M Contel
    Cancer Med, 2019-06-13;0(0):.  2019-06-13
  23. MASTL induces Colon Cancer progression and Chemoresistance by promoting Wnt/?-catenin signaling
    Authors: SB Uppada, S Gowrikumar, R Ahmad, B Kumar, B Szeglin, X Chen, JJ Smith, SK Batra, AB Singh, P Dhawan
    Mol. Cancer, 2018-08-01;17(1):111.  2018-08-01
  24. Acquired resistance to AZD9291 as an upfront treatment is dependent on ERK signaling in a preclinical model
    Authors: BM Ku, MK Choi, JM Sun, SH Lee, JS Ahn, K Park, MJ Ahn
    PLoS ONE, 2018-04-11;13(4):e0194730.  2018-04-11
  25. Ferric citrate and ferric EDTA but not ferrous sulfate drive amphiregulin-mediated activation of the MAP kinase ERK in gut epithelial cancer cells
    Authors: NM Scheers, DIA Pereira, N Faria, JJ Powell
    Oncotarget, 2018-03-30;9(24):17066-17077.  2018-03-30
  26. Melatonin attenuates hypoxia-induced epithelial-mesenchymal transition and cell aggressive via Smad7/ CCL20 in glioma.
    Authors: Chen X, Wang Z, Ma H, Zhang S, Yang H, Wang H, Fang Z
    Oncotarget, 2017-08-24;8(55):93580-93592.  2017-08-24
  27. CCL20 mediates RANK/RANKL-induced epithelial-mesenchymal transition in endometrial cancer cells
    Oncotarget, 2016-05-03;7(18):25328-39.  2016-05-03
  28. Small cell lung cancer: Circulating tumor cells of extended stage patients express a mesenchymal-epithelial transition phenotype
    Authors: G Hamilton, M Hochmair, B Rath, L Klameth, R Zeillinger
    Cell Adh Migr, 2016-02-26;10(4):360-7.  2016-02-26

FAQs

  1. Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

    • The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment. 

  2. Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?

    • We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.

View all Proteome Profiler Antibody Array FAQs
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Reviews for Proteome Profiler Human XL Oncology Array

Average Rating: 4.9 (Based on 7 Reviews)

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Proteome Profiler Human XL Oncology Array
By Anonymous on 12/06/2021
Proteome Profiler Human XL Oncology Array
By Anonymous on 03/25/2021
Proteome Profiler Human XL Oncology Array ARY026
Proteome Profiler Human XL Oncology Array
By Anonymous on 01/29/2021
Proteome Profiler Human XL Oncology Array ARY026
Proteome Profiler Human XL Oncology Array
By Anonymous on 04/20/2020
Proteome Profiler Human XL Oncology Array ARY026
Proteome Profiler Human XL Oncology Array
By Elad Prinz on 03/05/2020
Proteome Profiler Human XL Oncology Array ARY026
Proteome Profiler Human XL Oncology Array
By Anonymous on 07/31/2018
Proteome Profiler Human XL Oncology Array ARY026
Proteome Profiler Human XL Oncology Array
By Anonymous on 03/08/2017
Proteome Profiler Human XL Oncology Array ARY026