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Mouse VEGFR2/KDR/Flk-1 Antibody

Catalog #: MAB4431
6 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB4431-SP
MAB4431-100
MAB4431-500
VEGFR2/KDR/Flk‑1 Inhibition of VEGF-dependent Cell Proliferation and Neutralization by Mouse VEGFR2/KDR/Flk‑1 Antibody.
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Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse VEGFR2/KDR/Flk-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse VEGFR2 in Western blots.
Source
Monoclonal Rat IgG2A Clone # 91202
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VEGFR2
Ala20-Glu762
Accession # P35918
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample

Mouse VEGFR2/Flk-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse VEGFR2/KDR/Flk-1 Biotinylated Antibody (Catalog # BAF644)

Standard: Recombinant Mouse VEGFR2/Flk-1 Fc Chimera Protein (Catalog # 443-KD)

Neutralization
Measured by its ability to neutralize VEGFR2/KDR/Flk‑1-mediated inhibition of proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 0.05-0.15 µg/mL in the presence of 150 ng/mL Recombinant Mouse VEGFR2/KDR/Flk‑1 Fc Chimera and 5 ng/mL Recombinant Mouse VEGF164.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization VEGFR2/KDR/Flk‑1 Inhibition of VEGF-dependent Cell Proliferation and Neutralization by Mouse VEGFR2/KDR/Flk‑1 Antibody. View Larger

VEGFR2/KDR/Flk‑1 Inhibition of VEGF-dependent Cell Proliferation and Neutralization by Mouse VEGFR2/KDR/Flk‑1 Antibody. Recombinant Mouse VEGFR2/KDR/Flk-1 Fc Chimera (Catalog # 443-KD) inhibits Recombinant Mouse VEGF164(Catalog # 493-MV) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse VEGF164(5 ng/mL) activity elicited by Recombinant Mouse VEGFR2/KDR/Flk-1 Fc Chimera (150 ng/mL) is neutralized (green line) by increasing concentrations of Mouse VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB4431). The ND50 is typically 0.05-0.15 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR2/KDR/Flk-1

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mouse VEGFR2 cDNA encodes a 1367 amino acid (aa) precursor protein with a 19 aa signal peptide. Mature VEGFR2 is composed of a 743 aa extracellular domain, a 22 aa transmembrane domain, and a 583 aa cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

References
  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
  2. Achen, M.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
Long Name
Vascular Endothelial Growth Factor Receptor 2
Entrez Gene IDs
3791 (Human); 16542 (Mouse)
Alternate Names
CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2

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Citations for Mouse VEGFR2/KDR/Flk-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. A glycan-based approach to therapeutic angiogenesis
    Authors: Jie Shi Chua, Vy M. Tran, Mausam Kalita, Maritza V. Quintero, Orlando Antelope, Geethu Muruganandam et al.
    PLOS ONE
  2. Fibrotic Changes and Endothelial-to-Mesenchymal Transition Promoted by VEGFR2 Antagonism Alter the Therapeutic Effects of VEGFA Pathway Blockage in a Mouse Model of Choroidal Neovascularization
    Authors: FA Rossato, Y Su, A Mackey, YSE Ng
    Cells, 2020-09-09;9(9):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. Mycobacterium tuberculosis exploits the formation of new blood vessels for its dissemination
    Sci Rep, 2016-09-12;6(0):33162.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. VEGF-A modulates expression of inhibitory checkpoints on CD8+ T cells in tumors.
    Authors: Voron T, Colussi O, Marcheteau E, Pernot S, Nizard M, Pointet A, Latreche S, Bergaya S, Benhamouda N, Tanchot C, Stockmann C, Combe P, Berger A, Zinzindohoue F, Yagita H, Tartour E, Taieb J, Terme M
    J Exp Med, 2015-01-19;212(2):139-48.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Spontaneous CNV in a novel mutant mouse is associated with early VEGF-A-driven angiogenesis and late-stage focal edema, neural cell loss, and dysfunction.
    Authors: Nagai, Norihiro, Lundh von Leithner, Pete, Izumi-Nagai, Kanako, Hosking, Brett, Chang, Bo, Hurd, Ron, Adamson, Peter, Adamis, Anthony, Foxton, Richard, Ng, Yin Shan, Shima, David T
    Invest Ophthalmol Vis Sci, 2014-05-20;55(6):3709-19.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  6. alpha2beta1 integrin expression in the tumor microenvironment enhances tumor angiogenesis in a tumor cell-specific manner.
    Authors: Zhang Z, Ramirez NE, Yankeelov TE, Li Z, Ford LE, Qi Y, Pozzi A, Zutter MM
    Blood, 2007-11-27;111(4):1980-8.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr, Neutralization, Western Blot

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