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Mouse VEGFR2/KDR/Flk-1 Biotinylated Antibody

Catalog #: BAF644
2 Citations Datasheet
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BAF644
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse VEGFR2/KDR/Flk-1 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse VEGFR2/KDR/Flk-1 in ELISAs and Western blots. In sandwich ELISAs, less than 0.5% cross-reactivity with recombinant human VEGFR2, recombinant mouse (rm) VEGF164, rmVEGFR1, rmVEGFR3, rmVEGF-B, and rmVEGF-D is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VEGFR2/KDR/Flk-1
Ala20-Glu762
Accession # P35918
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse VEGFR2/KDR/Flk‑1 Fc Chimera (Catalog # 443-KD)
Flow Cytometry
2.5 µg/106 cells
bEnd.3 mouse endothelioma cell line

Mouse VEGFR2/Flk-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse VEGFR2/KDR/Flk-1 Antibody (Catalog # MAB4431)

Standard: Recombinant Mouse VEGFR2/Flk-1 Fc Chimera Protein (Catalog # 443-KD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR2/KDR/Flk-1

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.

Mouse VEGFR2 cDNA encodes a 1367 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGFR2 is composed of a 743 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 583 aa residue cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

References
  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
  2. Achen, M.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
Long Name
Vascular Endothelial Growth Factor Receptor 2
Entrez Gene IDs
3791 (Human); 16542 (Mouse)
Alternate Names
CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2

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Citations for Mouse VEGFR2/KDR/Flk-1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. TBX2-positive cells represent a multi-potent mesenchymal progenitor pool in the developing lung
    Authors: I Wojahn, TH Lüdtke, VM Christoffe, MO Trowe, A Kispert
    Respir. Res., 2019-12-23;20(1):292.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. A novel two-step procedure to expand cardiac Sca-1+ cells clonally.
    Authors: Tang YL, Shen L, Qian K, Phillips MI
    Biochem. Biophys. Res. Commun., 2007-06-11;359(4):877-83.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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