Mouse VCAM-1/CD106 Antibody Summary
Phe25-Glu698 (predicted)
Accession # P29533
Applications
Mouse VCAM-1/CD106 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse VCAM‑1/CD106 by Western Blot. Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse VCAM-1/CD106 Monoclonal Antibody (Catalog # MAB6432) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for VCAM-1/ CD106 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of VCAM‑1/CD106 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse VCAM-1/CD106 Monoclonal Antibody (Catalog # MAB6432, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
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Detection of Mouse VCAM‑1/CD106 by Simple WesternTM. Simple Western lane view shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line, loaded at 0.2 mg/mL. A specific band was detected for VCAM-1/CD106 at approximately 118 kDa (as indicated) using 100 µg/mL of Rat Anti-Mouse VCAM-1/CD106 Monoclonal Antibody (Catalog # MAB6432) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Mouse VCAM-1/CD106 by Flow Cytometry Analysis of IL-4 influence on expression of selected cell membrane proteins in ESCs. (A) Expression of Cdh15, Vcam1, Itga4, and Itgb1 in undifferentiated and differentiating ESCs cultured with or without exogenous IL-4. beta -actin was used as a reference gene. RQ = 1 for the expression level detected in 13.5-day-old mouse embryo. Data are presented as means of three independent experiments with standard deviations; * p < 0.05; ** p < 0.01. (B) Representative histograms presenting percentage of cells synthesizing VCAM1 in unstained control (upper), control (middle), or IL-4-treated (bottom) ESCs after 2 days of treatment, analyzed with flow cytometry. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31412558), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VCAM-1/CD106
VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.
Product Datasheets
Citations for Mouse VCAM-1/CD106 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Interleukin 4 Moderately Affects Competence of Pluripotent Stem Cells for Myogenic Conversion.
Authors: Swierczek-Lasek B, Neska J et al.
Int J Mol Sci
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Stress-Induced Changes in Bone Marrow Stromal Cell Populations Revealed through Single-Cell Protein Expression Mapping
Authors: N Severe, NM Karabacak, K Gustafsson, N Baryawno, G Courties, Y Kfoury, KD Kokkaliari, C Rhee, D Lee, EW Scadden, JE Garcia-Rob, T Brouse, M Nahrendorf, M Toner, DT Scadden
Cell Stem Cell, 2019-07-03;0(0):.
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Diabetic Endothelial Cell Glycogen Synthase Kinase 3? Activation Induces VCAM1 Ectodomain Shedding
Authors: Brishti MA, Raghavan S, Lamar K et al.
Int J Mol Sci
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Immunohistochemical study for the expression of leukocyte adhesion molecules, and FGF23 and ACE2 in P. gingivalis LPS-induced diabetic nephropathy
Authors: K Kajiwara, Y Sawa, T Fujita, S Tamaoki
Bmc Nephrology, 2021-01-06;22(1):3.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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