Mouse VCAM‑1/CD106 Biotinylated Antibody

Catalog #: BAM6433 Datasheet

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BAM6433

All VCAM-1/CD106 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse VCAM‑1/CD106 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse VCAM-1/CD106 in ELISAs and Western blots. In ELISAs, no cross-reactivity or interference was observed with recombinant human VCAM‑1, rmICAM-1, rmE-Selectin, rmL-Selectin, or rmP-Selectin.

Source

Monoclonal Rat IgG_2A Clone # 112731

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse VCAM-1
Phe25-Glu698
Accession # P29533

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse VCAM‑1/CD106 Fc Chimera (Catalog # 643-VM)

Mouse VCAM-1/CD106 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Mouse VCAM‑1/CD106 Antibody (Catalog # MAB6432)

Standard: Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein, CF (Catalog # 643-VM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VCAM-1/CD106

VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha ₄ beta ₇. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha ₄ beta ₇ interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

Long Name

Vascular Cell Adhesion Molecule 1

Entrez Gene IDs

7412 (Human); 22329 (Mouse)

Alternate Names

CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1