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Mouse Pro-MMP‑9 Biotinylated Antibody

Catalog #: BAM909
3 Citations Datasheet
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BAM909
Product Details
Citations (3)
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Mouse Pro-MMP‑9 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Pro-MMP-9 in direct ELISAs. Does not detect the mature form of MMP-9. In sandwich ELISAs, no cross-reactivity was observed with recombinant human (rh) MMP-1, rhMMP-2, rhMMP-3, rhMMP-8, rhMMP-10, rhMMP-12, and rhMMP‑13.
Source
Monoclonal Rat IgG2A Clone # 116125
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MMP‑9
Ala20-Pro730
Accession # P41245.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample

Mouse Pro-MMP-9 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Mouse Pro-MMP‑9 Antibody (Catalog # MAB9092)

Standard: Recombinant Mouse MMP-9 Protein, CF (Catalog # 909-MM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-9

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 may be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain. Compared to the human MMP-9 (R&D Systems, Catalog # 911-MP), the mouse enzyme contains extra sequences in the linker region and in the hemopexin-like domain, respectively.

Long Name
Matrix Metalloproteinase 9
Entrez Gene IDs
4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)
Alternate Names
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase

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Citations for Mouse Pro-MMP‑9 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Gelatinolytic activity of autocrine matrix metalloproteinase-9 leads to endothelial de-arrangement in Moyamoya disease
    Authors: KG Blecharz-L, V Prinz, M Burek, D Frey, T Schenkel, SM Krug, M Fromm, P Vajkoczy
    J. Cereb. Blood Flow Metab., 2018-04-10;0(0):271678X187684.
    Species: Human
    Sample Types: Tissue Lysates
    Applications: Western Blot
  2. Brucella abortus induces TNF-alpha-dependent astroglial MMP-9 secretion through mitogen-activated protein kinases.
    Authors: Miraglia M, Scian R, Samartino C, Barrionuevo P, Rodriguez A, Ibanez A, Coria L, Velasquez L, Baldi P, Cassataro J, Delpino M, Giambartolomei G
    J Neuroinflammation, 2013-04-12;10(0):47.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  3. Compartment-specific remodeling of splenic micro-architecture during experimental visceral leishmaniasis.
    Authors: Yurdakul P, Dalton J, Beattie L, Brown N, Erguven S, Maroof A, Kaye PM
    Am. J. Pathol., 2011-04-30;179(1):23-9.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-P

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