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Mouse Pro-MMP‑9 Antibody

Catalog #: MAB9092
5 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB9092
MAB9092-SP
Product Details
Citations (5)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Pro-MMP‑9 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Pro-MMP-9 in ELISAs. This antibody does not recognize the mature form of Pro-MMP-9.
Source
Monoclonal Rat IgG2A Clone # 116134
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MMP‑9
Ala20-Pro730
Accession # P41245.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample

Mouse Pro-MMP-9 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Pro-MMP‑9 Biotinylated Antibody (Catalog # BAM909)

Standard: Recombinant Mouse MMP-9 Protein, CF (Catalog # 909-MM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-9

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. Structurally, MMP-9 may be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin-binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline-rich linker region, and a carboxyl terminal hemopexin-like domain. Compared to the human MMP-9 (Catalog # 911-MP), the mouse enzyme contains extra sequences in the linker region and in the hemopexin-like domain, respectively.

Long Name
Matrix Metalloproteinase 9
Entrez Gene IDs
4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)
Alternate Names
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase

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Citations for Mouse Pro-MMP‑9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. 89Zr-pro-MMP-9 F(ab')2 detects colitis induced intestinal and kidney fibrosis
    Authors: N Dmochowska, W Tieu, MD Keller, CA Hollis, MA Campaniell, C Mavrangelo, P Takhar, PA Hughes
    Sci Rep, 2020-11-23;10(1):20372.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  2. G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
    Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
    J Immunol, 2013-04-15;190(10):5329-36.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Capture)
  3. Brucella abortus induces TNF-alpha-dependent astroglial MMP-9 secretion through mitogen-activated protein kinases.
    Authors: Miraglia M, Scian R, Samartino C, Barrionuevo P, Rodriguez A, Ibanez A, Coria L, Velasquez L, Baldi P, Cassataro J, Delpino M, Giambartolomei G
    J Neuroinflammation, 2013-04-12;10(0):47.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  4. Matrix metalloproteinase 9 facilitates West Nile virus entry into the brain.
    Authors: Wang P, Dai J, Bai F, Kong KF, Wong SJ, Montgomery RR, Madri JA, Fikrig E
    J. Virol., 2008-07-16;82(18):8978-85.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Matrix metalloproteinase-8 facilitates neutrophil migration through the corneal stromal matrix by collagen degradation and production of the chemotactic peptide Pro-Gly-Pro.
    Authors: Lin M, Jackson P, Tester AM, Diaconu E, Overall CM, Blalock JE, Pearlman E
    Am. J. Pathol., 2008-06-13;173(1):144-53.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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