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Mouse CD117/c-kit Biotinylated Antibody

Catalog #: BAM1356
1 Citation Datasheet
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BAM1356
Product Details
Citations (1)
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Mouse CD117/c-kit Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse SCF R/c‑kit in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 180627
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit
Met1-Thr519
Accession # P05532
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse SCF R/c-kit under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
Lineage depleted mouse bone marrow
Immunohistochemistry
8-25 µg/mL
Perfusion fixed frozen sections of mouse brain (cerebellum)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD117/c-kit

Stem cell factor receptor (CD117, the gene product of the c-kit protooncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. It is a transmembrane tyrosine kinase that is expressed on endothelial cells, mast cells, megakaryocytes, stem cells and multiple embryonic cells, such as melanoblasts and primordial germ cells.

Long Name
Stem Cell Factor Receptor
Entrez Gene IDs
3815 (Human); 16590 (Mouse); 64030 (Rat)
Alternate Names
CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFR; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

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Citation for Mouse CD117/c-kit Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Role of stem cell factor and bone marrow-derived fibroblasts in airway remodeling.
    Authors: Dolgachev VA, Ullenbruch MR, Lukacs NW, Phan SH
    Am. J. Pathol., 2009-01-15;174(2):390-400.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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