Human Phospho-CD117/c-kit (Y730) Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Phospho-CD117/ c‑kit (Y730) by Western Blot. Western blot shows lysates of MO7e human megakaryocytic leukemic cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human SCF/c-kit Ligand (Catalog # 255-SC) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-CD117/c-kit (Y730) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3527), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-CD117/c-kit (Y730) at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human CD117/c-kit by Simple WesternTM. Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit at approximately 177 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-CD117/c-kit (Y730) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3527). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD117/c-kit
Stem cell factor receptor (CD117, the gene product of the c-kit proto-oncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. The human stem cell factor receptor cDNA encodes a 972 amino acid (aa) residue precursor membrane protein with a 25 aa residue signal peptide (experimentally determined), a 495 aa residue extracellular domain, a 23 aa residue transmembrane segment and a 429 aa residue cytoplasmic domain. Stem cell factor receptor is a member of the type III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, Flt-3, PDGF and VEGF. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. SCF binding induces receptor homodimerization and signal transduction. SCF receptor is expressed in hematopoietic progenitor cells, normal B- and T-lymphocyte progenitor cells, mast cells, germ cells, melanocytes, neurons, glial cells, placenta, kidney, lung and gut. In addition, SCF receptor expression has also been reported in a number of human tumor cell lines. SCF receptor can be proteolytically cleaved from the cell surface and high levels of soluble SCF receptor has been detected in cell conditioned medium and human plasma. Recombinant soluble SCF receptor binds SCF with high affinity and is a potent SCF antagonist.
- Broudy, V. (1997) Blood 90:1345.
- Vliagoftis, H. et al. (1997) J. Allergy Clin. Immunol. 100:435.
Product Datasheets
Citations for Human Phospho-CD117/c-kit (Y730) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Transmembrane stem cell factor protein therapeutics enhance revascularization in ischemia without mast cell activation
Authors: E Takematsu, M Massidda, J Auster, PC Chen, B Im, S Srinath, S Canga, A Singh, M Majid, M Sherman, A Dunn, A Graham, P Martin, AB Baker
Nature Communications, 2022-05-06;13(1):2497.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Engineering Stem Cell Factor Ligands with Different c-Kit Agonistic Potencies
Authors: T Tilayov, T Hingaly, Y Greenshpan, S Cohen, B Akabayov, R Gazit, N Papo
Molecules, 2020-10-21;25(20):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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