Human/Mouse CD117/c-kit Antibody Summary
Gln25-Thr519 (Ala207Glu)
Accession # P05532
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
CD117/c-kit in Mouse Embryo. CD117/c-kit was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Human and Mouse CD117/c-kit by Western Blot. Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9-2 mouse mast cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for CD117/c-kit at approximately 135, 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Mouse CD117/c-kit by Simple WesternTM. Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line and MC/9-2 mouse mast cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit at approximately 150-165 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Mouse CD117/c-kit by Immunocytochemistry/Immunofluorescence Localization of MAFB in mouse testes.(A) Localization of MAFB in E18.5 mouse testes. Double immunostaining of MAFB with E-cadherin (ECAD), GATA4, or STAR is shown. Nuclei were counterstained with DAPI. The color of each marker is indicated above the images. G; germ cells. S; Sertoli cells. L; Leydig cells. MAFB was specifically detected in Leydig cells and Sertoli cells. (B) Localization of MAFB in adult mouse testes. Double immunostaining of MAFB with E-cadherin (ECAD), KIT, SCP3, PNA Lectin, or vimentin is shown. Nuclei were counterstained with DAPI. The color of each marker is indicated above the images. All seminiferous tubules shown represent stage VII. US; undifferentiated spermatogonia. DS; differentiated spermatogonia. P; pachytene spermatocytes. Sp; spermatids. S; Sertoli cells. L; Leydig cells. MAFB was specifically detected in Leydig cells, Sertoli cells, and pachytene spermatocytes. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0190800), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse CD117/c-kit by Immunocytochemistry/Immunofluorescence Spermatogenesis development of adult Mafb-cKO mice.Six-week-old mice were injected with tamoxifen and testes of cKO mice at 3- and 8-months of age were examined compared to those of age-matched controls (n = 4 for each group). (A) Testicular sections stained with Periodic acid-Schiff (PAS). (B) Immunostaining with various testicular cell types; undifferentiated spermatogonia (GFRA1 and PLZF), differentiated spermatogonia (KIT+ inside the tubule), spermatocytes (SCP3), spermatids (PNA-Lectin), Sertoli cells (SOX9 and Vimentin), and Leydig cells (KIT+ outside the tubule and STAR). The color of markers is indicated in the left boxes in panels. Colored arrows corresponding to the boxed markers are shown. (C) The expression level of the genes involved in testis function and germ cell development. The marker genes representing undifferentiated spermatogonia (Nanos3), differentiated spermatogonia (c-Kit), undifferentiated and differentiated spermatogonia (Sohlh1), differentiated spermatogonia and preleptotene spermatocytes (Stra8), spermatids (Prm2), Leydig cells (Hsd3b1), and Sertoli cells (Sox9) were analyzed by qRT-PCR. Each reaction was performed in duplicate for each gene. The data represent the means±SEM and are shown as relative mRNA expression after normalization to Hprt. *P<0.05. (D) The proportion of seminiferous stages: I-III, IV-VI, VII-VIII, and IX-XII from each genotype. *P<0.05. M; Month. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0190800), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse CD117/c-kit by Immunocytochemistry/Immunofluorescence ANXA8+ve/c-kit+ve luminal progenitor cells are mostly Ki67−ve.Co-immunofluorescence staining for ANXA8 (green), c-kit (red), and Ki67 (purple) in mouse mammary gland from virgin and mid-pregnant mice shows that ANXA8+ve cells express c-kit, but not Ki67. The Ki67 staining has been coloured purple for easier visualisation in the triple-merged image. These are representative images of at least three independent mice for each time point. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25803307), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CD117/c-kit in Human Mouse Embryos. Formalin-fixed paraffin-embedded tissue sections of mouse embryo were probed for CD117 mRNA (ACD RNAScope Probe, catalog # 314158; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-mouse CD117 polyclonal antibody (R&D Systems catalog # AF1356) at 5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in esophagus in mouse embryo.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD117/c-kit
Stem cell factor receptor (CD117, the gene product of the c-kit protooncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. It is a transmembrane tyrosine kinase that is expressed on endothelial cells, mast cells, megakaryocytes, stem cells and multiple embryonic cells, such as melanoblasts and primordial germ cells.
Product Datasheets
Citations for Human/Mouse CD117/c-kit Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Streptozotocin?induced diabetic mice exhibit reduced experimental choroidal neovascularization but not corneal neovascularization
Authors: G Liu, L Chen, Q Cai, H Wu, Z Chen, X Zhang, P Lu
Mol Med Rep, 2018-09-03;0(0):.
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Yeokwisan, a Standardized Herbal Formula, Enhances Gastric Emptying via Modulation of the Ghrelin Pathway in a Loperamide-induced Functional Dyspepsia Mouse Model
Authors: Seung-Ju Hwang, Jing-Hua Wang, Jin-Seok Lee, Hwa-Dong Lee, Tae-Joon Choi, Seo-Hyung Choi et al.
Frontiers in Pharmacology
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The germ cell-specific RNA binding protein RBM46 is essential for spermatogonial differentiation in mice
Authors: Peart NJ, Johnson TA, Lee S et al.
PLOS Genetics
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WNK1 is required during male pachynema to sustain fertility
Authors: Ru-pin Alicia Chi, Xiaojiang Xu, Jian-Liang Li, Xin Xu, Guang Hu, Paula Brown et al.
iScience
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Different niches for stem cells carrying the same oncogenic driver affect pathogenesis and therapy response in myeloproliferative neoplasms
Authors: Elodie Grockowiak, Claudia Korn, Justyna Rak, Veronika Lysenko, Adrien Hallou, Francesca M. Panvini et al.
Nature Cancer
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Novel Designed Proteolytically Resistant VEGF-B186R127S Promotes Angiogenesis in Mouse Heart by Recruiting Endothelial Progenitor Cells
Authors: Rahul Mallick, Erika Gurzeler, Pyry I. Toivanen, Tiina Nieminen, Seppo Ylä-Herttuala
Frontiers in Bioengineering and Biotechnology
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Critical role of SDF-1 alpha -induced progenitor cell recruitment and macrophage VEGF production in the experimental corneal neovascularization
Authors: Gaoqin Liu, Peirong Lu, Longbiao Li, Hui Jin, Xuefei He, Naofumi Mukaida et al.
Mol Vis
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Lipid phosphatase SHIP-1 regulates chondrocyte hypertrophy and skeletal development
Authors: So EY, Sun C, Wu KQ et al.
J. Cell. Physiol.
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NANOS2 is a sequence-specific mRNA-binding protein that promotes transcript degradation in spermatogonial stem cells
Authors: Azzurra Codino, Tomasz Turowski, Louie N. van de Lagemaat, Ivayla Ivanova, Andrea Tavosanis, Christian Much et al.
iScience
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Identification of dynamic undifferentiated cell states within the male germline
Authors: HM La, JA Mäkelä, AL Chan, FJ Rossello, CM Nefzger, JMD Legrand, M De Seram, JM Polo, RM Hobbs
Nat Commun, 2018-07-19;9(1):2819.
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Transduction of Systemically Administered Adeno-Associated Virus in the Colonic Enteric Nervous System and c-Kit Cells of Adult Mice
Authors: Lixin Wang, Pu-Qing Yuan, Collin Challis, Sripriya Ravindra Kumar, Yvette Taché
Frontiers in Neuroanatomy
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RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis
Authors: Liu C, Wang L, Li Y et al.
Nucleic acids research
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A Novel Population of Myeloid Cells Responding to Coxsackievirus Infection Assists in the Dissemination of Virus within the Neonatal CNS
Authors: Jenna M. Tabor-Godwin, Chelsea M. Ruller, Nolan Bagalso, Naili An, Robb R. Pagarigan, Stephanie Harkins et al.
Journal of Neuroscience
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The combined action of CTCF and its testis-specific paralog BORIS is essential for spermatogenesis
Authors: Rivero-Hinojosa S, Pugacheva EM, Kang S et al.
Nature Communications
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Differential RA responsiveness among subsets of mouse late progenitor spermatogonia
Authors: Shinnosuke Suzuki, John R. McCarrey, Brian P. Hermann
Reproduction
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The Impact of Activin A on Fetal Gonocytes: Chronic Versus Acute Exposure Outcomes
Authors: Sarah C. Moody, Penny A. F. Whiley, Patrick S. Western, Kate L. Loveland
Front Endocrinol (Lausanne)
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Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis
Authors: Hirano K, Nonami Y, Nakamura Y et al.
Communications Biology
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Oocytes can efficiently repair DNA double-strand breaks to restore genetic integrity and protect offspring health
Authors: Jessica M. Stringer, Amy Winship, Nadeen Zerafa, Matthew Wakefield, Karla Hutt
Proceedings of the National Academy of Sciences
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KDM1A/LSD1 regulates the differentiation and maintenance of spermatogonia in mice
Authors: Dexter A. Myrick, Michael A. Christopher, Alyssa M. Scott, Ashley K. Simon, Paul G. Donlin-Asp, William G. Kelly et al.
PLOS ONE
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Dystrophin Deficiency Causes Progressive Depletion of Cardiovascular Progenitor Cells in the Heart
Authors: Jelinkova S, Sleiman Y, FojtIk P et al.
International Journal of Molecular Sciences
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WNT signaling in pre-granulosa cells is required for ovarian folliculogenesis and female fertility
Authors: Okiko Habara, Catriona Y. Logan, Masami Kanai-Azuma, Roeland Nusse, Hinako M. Takase
Development
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Defective germline reprogramming rewires the spermatogonial transcriptome
Authors: Lina Vasiliauskaitė, Rebecca V. Berrens, Ivayla Ivanova, Claudia Carrieri, Wolf Reik, Anton J. Enright et al.
Nature Structural & Molecular Biology
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Distinct expression patterns of Notch ligands, Dll1 and Dll4, in normal and inflamed mice intestine
Authors: Hiromichi Shimizu, Ryuichi Okamoto, Go Ito, Satoru Fujii, Toru Nakata, Kohei Suzuki et al.
PeerJ
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Efficient Surface Immobilization of Chemically Modified Hyaluronans for Enhanced Bioactivity and Survival of In Vitro-Cultured Embryonic Salivary Gland Mesenchymal Cells
Authors: Sang-woo Lee, Junchul Kim, Xin Cong, Guang-Yan Yu, Ji Hyun Ryu, Kyungpyo Park
Polymers (Basel)
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A direct role for murine Cdx proteins in the trunk neural crest-gene regulatory network
Authors: Oraly Sanchez-Ferras, Guillaume Bernas, Omar Farnos, Aboubacrine M. Touré, Ouliana Souchkova, Nicolas Pilon
Development
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Mast Cells Are the Trigger of Small Vessel Disease and Diastolic Dysfunction in Diabetic Obese Mice
Authors: Sarah Guimbal, Lauriane Cornuault, Paul Rouault, Pierre-Louis Hollier, Candice Chapouly, Marie-Lise Bats et al.
Arteriosclerosis, Thrombosis, and Vascular Biology
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Rap1 signal modulators control the maintenance of hematopoietic progenitors in bone marrow and adult long‐term hematopoiesis
Authors: Takahiko Imai, Hiroki Tanaka, Yoko Hamazaki, Nagahiro Minato
Cancer Science
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Stimulation of C-Kit+ Retinal Progenitor Cells by Stem Cell Factor Confers Protection Against Retinal Degeneration
Authors: Xi Chen, Shanshan Li, Xiaoli Liu, Jingjie Zhao, Lanting Wu, Ran You et al.
Frontiers in Pharmacology
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Identification of primordial germ cell-like cells as liver metastasis initiating cells in mouse tumour models
Authors: Liu C, Ma Z, Cai Z et Al.
Cell Discov
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GILZ-dependent modulation of mTORC1 regulates spermatogonial maintenance
Authors: Hue M. La, Ai-Leen Chan, Julien M. D. Legrand, Fernando J. Rossello, Christina G. Gangemi, Antonella Papa et al.
Development
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Sertoli cells are the unique source of stem cell factor for spermatogenesis
Authors: Yi Jacky Peng, Xinyu Thomas Tang, Hui Sophie Shu, Wenjie Dong, Hongfang Shao, Bo O. Zhou
Development
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Vagal Intramuscular Arrays: The Specialized Mechanoreceptor Arbors That Innervate the Smooth Muscle Layers of the Stomach Examined in the Rat
Authors: Terry L. Powley, Cherie N. Hudson, Jennifer L. McAdams, Elizabeth A. Baronowsky, Robert J. Phillips
Journal of Comparative Neurology
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Cellular and subcellular localization of cholecystokinin (CCK)-1 receptors in the pancreas, gallbladder, and stomach of mice
Authors: Kohtarou Konno, Hiromi Takahashi-Iwanaga, Motokazu Uchigashima, Kyoko Miyasaka, Akihiro Funakoshi, Masahiko Watanabe et al.
Histochemistry and Cell Biology
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CENP-A is essential for cardiac progenitor cell proliferation
Authors: Michael McGregor, Nirmala Hariharan, Anya Y Joyo, Robert L Margolis, Mark A Sussman
Cell Cycle
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Neural Crest–Derived Stem Cells Migrate and Differentiate Into Cardiomyocytes After Myocardial Infarction
Authors: Yuichi Tamura, Keisuke Matsumura, Motoaki Sano, Hidenori Tabata, Kensuke Kimura, Masaki Ieda et al.
Arteriosclerosis, Thrombosis, and Vascular Biology
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Adeno-associated virus-9 reverses delayed gastric emptying of solids in diabetic mice.
Authors: Li X, Ji S et al.
Neurogastroenterol Motil
Species: Mouse
Sample Types:
Applications: Simple Western -
Conditional loss of Brca1 in oocytes causes reduced litter size, ovarian reserve depletion and impaired oocyte in vitro maturation with advanced reproductive age in mice
Authors: Winship, AL;Alesi, LR;Stringer, JM;Cao, Y;Lewis, YM;Tu, L;Swindells, EOK;Giridharan, S;Cai, X;Griffiths, MJ;Zerafa, N;Gilham, L;Hickey, M;Hutt, KJ;
EBioMedicine
Species: Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Inhibition of EZH2 Reduces Aging-Related Decline in Interstitial Cells of Cajal of the Mouse Stomach
Authors: Taheri, N;Choi, EL;Thuy Nguyen, VT;Zhang, Y;Huynh, NM;Kellogg, TA;van Wijnen, AJ;Ordog, T;Hayashi, Y;
Cellular and molecular gastroenterology and hepatology
Species: Transgenic Mouse
Sample Types: Cell Lysates, Tissue Homogenates
Applications: Western Blot -
Spatial heterogeneity of bone marrow endothelial cells unveils a distinct subtype in the epiphysis
Authors: Iga, T;Kobayashi, H;Kusumoto, D;Sanosaka, T;Fujita, N;Tai-Nagara, I;Ando, T;Takahashi, T;Matsuo, K;Hozumi, K;Ito, K;Ema, M;Miyamoto, T;Matsumoto, M;Nakamura, M;Okano, H;Shibata, S;Kohyama, J;Kim, KK;Takubo, K;Kubota, Y;
Nature cell biology
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
SRSF1-mediated alternative splicing is required for spermatogenesis
Authors: Lei, WL;Li, YY;Du, Z;Su, R;Meng, TG;Ning, Y;Hou, G;Schatten, H;Wang, ZB;Han, Z;Sun, F;Qian, WP;Liu, C;Sun, QY;
International journal of biological sciences
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
An organoid-based CRISPR-Cas9 screen for regulators of intestinal epithelial maturation and cell fate
Authors: Hansen, SL;Larsen, HL;Pikkupeura, LM;Maciag, G;Guiu, J;Müller, I;Clement, DL;Mueller, C;Johansen, JV;Helin, K;Lerdrup, M;Jensen, KB;
Science advances
Species: Mouse
Sample Types: Organoids
Applications: IHC -
Transcriptional metabolic reprogramming implements meiotic fate decision in mouse testicular germ cells
Authors: Zhang, X;Liu, Y;Sosa, F;Gunewardena, S;Crawford, PA;Zielen, AC;Orwig, KE;Wang, N;
Cell reports
Species: Mouse
Sample Types: Protein, Whole Cells, Whole Tissue
Applications: ICC/IF, IHC, Western Blot -
RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis
Authors: Liu C, Wang L, Li Y et al.
Nucleic acids research
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Insulin-like growth factor1 preserves gastric pacemaker cells and motor function in aging via ERK1/2 activation
Authors: Nguyen, VTT;Taheri, N;Choi, EL;Kellogg, TA;Linden, DR;Hayashi, Y;
Cellular and molecular gastroenterology and hepatology
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Polycomb repressive complex 1.1 coordinates homeostatic and emergency myelopoiesis
Authors: Nakajima-Takagi, Y;Oshima, M;Takano, J;Koide, S;Itokawa, N;Uemura, S;Yamashita, M;Andoh, S;Aoyama, K;Isshiki, Y;Shinoda, D;Saraya, A;Arai, F;Yamaguchi, K;Furukawa, Y;Koseki, H;Ikawa, T;Iwama, A;
eLife
Species: Transgenic Mouse, Mouse
Sample Types: Whole Tissue
Applications: IHC -
Osteoprogenitor-GMP crosstalk underpins solid tumor-induced systemic immunosuppression and persists after tumor removal
Authors: Hao, X;Shen, Y;Chen, N;Zhang, W;Valverde, E;Wu, L;Chan, HL;Xu, Z;Yu, L;Gao, Y;Bado, I;Michie, LN;Rivas, CH;Dominguez, LB;Aguirre, S;Pingel, BC;Wu, YH;Liu, F;Ding, Y;Edwards, DG;Liu, J;Alexander, A;Ueno, NT;Hsueh, PR;Tu, CY;Liu, LC;Chen, SH;Hung, MC;Lim, B;Zhang, XH;
Cell stem cell
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse
Authors: A Uchida, K Imaimatsu, H Suzuki, X Han, H Ushioda, M Uemura, K Imura-Kish, R Hiramatsu, HM Takase, Y Hirate, A Ogura, M Kanai-Azum, A Kudo, Y Kanai
Nature Communications, 2022-12-21;13(1):7860.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
New C3H KitN824K/WT cancer mouse model develops late-onset malignant mammary tumors with high penetrance
Authors: T Klein-Rode, K Micklich, A Sanz-Moren, M Tost, J Calzada-Wa, T Adler, M Klaften, S Sabrautzki, B Aigner, M Kraiger, V Gailus-Dur, H Fuchs, German Mou, A Gründer, H Pahl, E Wolf, M Hrabe de A, B Rathkolb
Scientific Reports, 2022-11-17;12(1):19793.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Uncovering the emergence of HSCs in the human fetal bone marrow by single-cell RNA-seq analysis
Authors: Z Zheng, H He, XT Tang, H Zhang, F Gou, H Yang, J Cao, S Shi, Z Yang, G Sun, X Xie, Y Zeng, A Wen, Y Lan, J Zhou, B Liu, BO Zhou, T Cheng, H Cheng
Cell Stem Cell, 2022-11-03;29(11):1562-1579.e7.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The germ cell-specific RNA binding protein RBM46 is essential for spermatogonial differentiation in mice
Authors: Peart NJ, Johnson TA, Lee S et al.
PLOS Genetics
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Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis
Authors: Hirano K, Nonami Y, Nakamura Y et al.
Communications Biology
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Disruption of the crypt niche promotes outgrowth of mutated colorectal tumor stem cells
Authors: S Klingler, KS Hsu, G Hua, ML Martin, M Adileh, T Baslan, Z Zhang, PB Paty, Z Fuks, AM Brown, R Kolesnick
JCI Insight, 2022-03-08;7(5):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Bone marrow sinusoidal endothelium controls terminal erythroid differentiation and reticulocyte maturation
Authors: J Heil, V Olsavszky, K Busch, K Klapproth, C de la Torr, C Sticht, K Sandorski, J Hoffmann, H Schönhaber, J Zierow, M Winkler, CD Schmid, T Staniczek, DE Daniels, J Frayne, G Metzgeroth, D Nowak, S Schneider, M Neumaier, V Weyer, C Groden, HJ Gröne, K Richter, C Mogler, MM Taketo, K Schledzews, C Géraud, S Goerdt, PS Koch
Nature Communications, 2021-11-29;12(1):6963.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Differentiation of fetal hematopoietic stem cells requires ARID4B to restrict autocrine KITLG/KIT-Src signaling
Authors: IC Young, B Wu, J Andricovic, ST Chuang, R Li, A Tzatsos, RC Wu, MY Wu
Cell Reports, 2021-11-23;37(8):110036.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Cdc42 activity in Sertoli cells is essential for maintenance of spermatogenesis
Authors: A Heinrich, B Bhandary, SJ Potter, N Ratner, T DeFalco
Cell Reports, 2021-10-26;37(4):109885.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Regulation of intestinal immunity and tissue repair by enteric glia
Authors: F Progatzky, M Shapiro, SH Chng, B Garcia-Cas, CH Classon, S Sevgi, A Laddach, AC Bon-Frauch, R Lasrado, M Rahim, EM Amaniti, S Boeing, K Shah, LJ Entwistle, A Suárez-Bon, MS Wilson, B Stockinger, V Pachnis
Nature, 2021-10-20;599(7883):125-130.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Bone marrow CD73+ mesenchymal stem cells display increased stemness in vitro and promote fracture healing in vivo
Authors: K Kimura, M Breitbach, FA Schildberg, M Hesse, BK Fleischman
Bone Reports, 2021-09-29;15(0):101133.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Telocytes in the human ascending aorta: Characterization and exosome-related KLF-4/VEGF-A expression
Authors: T Aschacher, K Schmidt, O Aschacher, E Eichmair, U Baranyi, B Winkler, M Grabenwoeg, A Spittler, F Enzmann, B Messner, J Riebandt, G Laufer, M Bergmann, M Ehrlich
Journal of Cellular and Molecular Medicine, 2021-09-25;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The combined action of CTCF and its testis-specific paralog BORIS is essential for spermatogenesis
Authors: Rivero-Hinojosa S, Pugacheva EM, Kang S et al.
Nature Communications
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Indispensable role of Galectin-3 in promoting quiescence of hematopoietic stem cells
Authors: W Jia, L Kong, H Kidoya, H Naito, F Muramatsu, Y Hayashi, HY Hsieh, D Yamakawa, DK Hsu, FT Liu, N Takakura
Nature Communications, 2021-04-09;12(1):2118.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Role of transient receptor potential vanilloid subtype 2 in lower oesophageal sphincter in rat acid reflux oesophagitis
Authors: K Matsumoto, M Suenaga, Y Mizutani, K Matsui, A Yoshida, T Nakamoto, S Kato
Journal of pharmacological sciences, 2021-04-07;146(3):125-135.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
An mTORC1-dependent switch orchestrates the transition between mouse spermatogonial stem cells and clones of progenitor spermatogonia
Authors: S Suzuki, JR McCarrey, BP Hermann
Cell Reports, 2021-02-16;34(7):108752.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Low retinoic acid levels mediate regionalization of the Sertoli valve in the terminal segment of mouse seminiferous tubules
Authors: K Imura-Kish, A Uchida, N Tsunekawa, H Suzuki, HM Takase, Y Hirate, M Kanai-Azum, R Hiramatsu, M Kurohmaru, Y Kanai
Scientific Reports, 2021-01-13;11(1):1110.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Single-cell transcriptomics reveals zone-specific alterations of liver sinusoidal endothelial cells in cirrhosis
Authors: T Su, Y Yang, S Lai, J Jeong, Y Jung, M McConnell, T Utsumi, Y Iwakiri
Cellular and Molecular Gastroenterology and Hepatology, 2020-12-16;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer
Authors: N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit
Nat Commun, 2020-10-14;11(1):5171.
Species: Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development
Authors: EI Crosse, S Gordon-Key, S Rybtsov, A Binagui-Ca, H Felchle, NC Nnadi, K Kirschner, T Chandra, S Tamagno, DJ Webb, F Rossi, RA Anderson, A Medvinsky
Cell Stem Cell, 2020-09-17;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Defective development and microcirculation of intestine in Npr2 mutant mice
Authors: C Sogawa-Fuj, A Hanagata, Y Fujiwara, Y Ishida, H Tomiyasu, T Kunieda, H Nakatomi, M Hori
Sci Rep, 2020-09-08;10(1):14761.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
PIM1 Promotes Survival of Cardiomyocytes by Upregulating c-Kit Protein Expression
Authors: DE Ebeid, F Firouzi, CY Esquer, JM Navarrete, BJ Wang, NA Gude, MA Sussman
Cells, 2020-08-31;9(9):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Wnt-induced, TRP53-mediated Cell Cycle Arrest of Precursors Underlies Interstitial Cell of Cajal Depletion during Aging
Authors: Y Hayashi, DT Asuzu, MR Bardsley, GB Gajdos, SM Kvasha, DR Linden, RA Nagy, SA Saravanape, SA Syed, Y Toyomasu, H Yan, EN Chini, SJ Gibbons, TA Kellogg, K Khazaie, M Kuro-O, JM Espindola, MP Singh, JG Tidball, M Wehling-He, G Farrugia, T Ordog
Cell Mol Gastroenterol Hepatol, 2020-08-07;0(0):.
Species: Human, Mouse
Sample Types: Cell Lysates, Tissue Homogenates
Applications: Western Blot -
DAZL mediates a broad translational program regulating expansion and differentiation of spermatogonial progenitors
Authors: MM Mikedis, Y Fan, PK Nicholls, T Endo, EK Jackson, SA Cobb, DG de Rooij, DC Page
Elife, 2020-07-20;9(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Distinct Roles for Rac1 in Sertoli Cell Function during Testicular Development and Spermatogenesis
Authors: A Heinrich, SJ Potter, L Guo, N Ratner, T DeFalco
Cell Rep, 2020-04-14;31(2):107513.
Species: Mouse
Sample Types: Testes
Applications: IF -
Multipotency of mouse trophoblast stem cells
Authors: M Hou, J Han, G Li, MY Kwon, J Jiang, S Emani, ES Taglauer, JA Park, EB Choi, M Vodnala, YW Fong, SM Emani, IO Rosas, MA Perrella, X Liu
Stem Cell Res Ther, 2020-02-13;11(1):55.
Species: Human, Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Mesenchymal Niche-Specific Expression of Cxcl12 Controls Quiescence of Treatment-Resistant Leukemia Stem Cells
Authors: P Agarwal, S Isringhaus, H Li, AJ Paterson, J He, Á Gomariz, T Nagasawa, C Nombela-Ar, R Bhatia
Cell Stem Cell, 2019-03-21;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Regnase-1-mediated post-transcriptional regulation is essential for hematopoietic stem and progenitor cell homeostasis
Authors: H Kidoya, F Muramatsu, T Shimamura, W Jia, T Satoh, Y Hayashi, H Naito, Y Kunisaki, F Arai, M Seki, Y Suzuki, T Osawa, S Akira, N Takakura
Nat Commun, 2019-03-06;10(1):1072.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
DDX5 plays essential transcriptional and post-transcriptional roles in the maintenance and function of spermatogonia
Authors: JMD Legrand, AL Chan, HM La, FJ Rossello, ML Änkö, FV Fuller-Pac, RM Hobbs
Nat Commun, 2019;10(1):2278.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Esrp1 is a marker of mouse fetal germ cells and differentially expressed during spermatogenesis
Authors: S Saeidi, F Shapouri, RU de Iongh, F Casagranda, JM Sutherland, PS Western, EA McLaughlin, M Familari, GR Hime
PLoS ONE, 2018-01-11;13(1):e0190925.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis
Authors: Y Kawai, A Oda, Y Kanai, R Goitsuka
PLoS ONE, 2018-01-02;13(1):e0190702.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Cardiac Nestin+ Cells Derived from Early Stage of Dilated Cardiomyopathy Enhanced the Survival of the Doxorubicin-Injured Cardiac Muscle HL-1 Cells
Authors: D Xie, Y Liao, B Wu, Y Chen, W Lin, D Lu, S Gao, S Zhu, C Peng, MH Jiang
Int Heart J, 2018-01-01;59(1):180-189.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Estrogen induces c-Kit and an aggressive phenotype in a model of invasive lobular breast cancer
Authors: JC Harrell, TM Shroka, BM Jacobsen
Oncogenesis, 2017-11-27;6(11):2.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: Western Blot -
Multicolor quantitative confocal imaging cytometry
Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
Nat. Methods, 2017-11-13;15(1):39-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Live cell screening platform identifies PPAR? as a regulator of cardiomyocyte proliferation and cardiac repair
Authors: A Magadum, Y Ding, L He, T Kim, MD Vasudevara, Q Long, K Yang, N Wickramasi, HV Renikunta, N Dubois, G Weidinger, Q Yang, FB Engel
Cell Res., 2017-06-16;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
A transit-amplifying population underpins the efficient regenerative capacity of the testis
Authors: C Carrieri, S Comazzetto, A Grover, M Morgan, A Buness, C Nerlov, D O'Carroll
J. Exp. Med., 2017-05-01;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Concurrent Isolation of Three Distinct Cardiac Stem Cell Populations from a Single Human Heart Biopsy
Authors: MM Monsanto, KS White, T Kim, BJ Wang, K Fisher, K Ilves, FG Khalafalla, A Casillas, K Broughton, S Mohsin, WP Dembitsky, MA Sussman
Circ. Res., 2017-04-26;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Transcriptome of interstitial cells of Cajal reveals unique and selective gene signatures
Authors: MY Lee, SE Ha, C Park, PJ Park, R Fuchs, L Wei, BG Jorgensen, D Redelman, SM Ward, KM Sanders, S Ro
PLoS ONE, 2017-04-20;12(4):e0176031.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Fabrication of Synthetic Mesenchymal Stem Cells for the Treatment of Acute Myocardial Infarction in Mice
Authors: L Luo, J Tang, K Nishi, C Yan, PU Dinh, J Cores, T Kudo, J Zhang, TS Li, K Cheng
Circ. Res, 2017-03-15;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Paracrine GABA and insulin regulate pancreatic alpha cell proliferation in a mouse model of type 1 diabetes
Authors: AL Feng, YY Xiang, L Gui, G Kaltsidis, Q Feng, WY Lu
Diabetologia, 2017-03-09;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Radiation Exposure Decreases the Quantity and Quality of Cardiac Stem Cells in Mice
PLoS ONE, 2016-05-19;11(5):e0152179.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC -
Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes.
Authors: Liu Q, Yang R, Huang X, Zhang H, He L, Zhang L, Tian X, Nie Y, Hu S, Yan Y, Zhang L, Qiao Z, Wang Q, Lui K, Zhou B
Cell Res, 2015-12-04;26(1):119-30.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Embryonic Hematopoietic Progenitor Cells Reside in Muscle before Bone Marrow Hematopoiesis.
Authors: Tanaka Y, Inoue-Yokoo T, Kulkeaw K, Yanagi-Mizuochi C, Shirasawa S, Nakanishi Y, Sugiyama D
PLoS ONE, 2015-09-21;10(9):e0138621.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Nuclear Calcium/Calmodulin-dependent Protein Kinase II Signaling Enhances Cardiac Progenitor Cell Survival and Cardiac Lineage Commitment.
Authors: Quijada P, Hariharan N, Cubillo J, Bala K, Emathinger J, Wang B, Ormachea L, Bers D, Sussman M, Poizat C
J Biol Chem, 2015-08-31;290(42):25411-26.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Annexin A8 identifies a subpopulation of transiently quiescent c-kit positive luminal progenitor cells of the ductal mammary epithelium.
Authors: Iglesias J, Cairney C, Ferrier R, McDonald L, Soady K, Kendrick H, Pringle M, Morgan R, Martin F, Smalley M, Blyth K, Stein T
PLoS ONE, 2015-03-24;10(3):e0119718.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Gastric LTi cells promote lymphoid follicle formation but are limited by IRAK-M and do not alter microbial growth.
Authors: Shiu J, Piazuelo M, Ding H, Czinn S, Drakes M, Banerjee A, Basappa N, Kobayashi K, Fricke W, Blanchard T
Mucosal Immunol, 2015-01-21;8(5):1047-59.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Isolation and characterization of resident endogenous c-Kit+ cardiac stem cells from the adult mouse and rat heart.
Authors: Smith A, Lewis F, Aquila I, Waring C, Nocera A, Agosti V, Nadal-Ginard B, Torella D, Ellison G
Nat Protoc, 2014-06-19;9(7):1662-81.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
c-kit+ cells minimally contribute cardiomyocytes to the heart.
Authors: van Berlo J, Kanisicak O, Maillet M, Vagnozzi R, Karch J, Lin S, Middleton R, Marban E, Molkentin J
Nature, 2014-05-07;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Differential regulation of cellular senescence and differentiation by prolyl isomerase Pin1 in cardiac progenitor cells.
Authors: Toko, Haruhiro, Hariharan, Nirmala, Konstandin, Mathias, Ormachea, Lucia, McGregor, Michael, Gude, Natalie, Sundararaman, Balaji, Joyo, Eri, Joyo, Anya Y, Collins, Brett, Din, Shabana, Mohsin, Sadia, Uchida, Takafumi, Sussman, Mark A
J Biol Chem, 2013-12-27;289(9):5348-56.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Thymosin beta4 increases the potency of transplanted mesenchymal stem cells for myocardial repair.
Authors: Ye L, Zhang P, Duval S, Su L, Xiong Q, Zhang J
Circulation, 2013-09-10;128(11):S32-41.
Species: Porcine
Sample Types: Whole Tissue
Applications: IHC-P -
Generation of Functional Blood Vessels from a Single c-kit+ Adult Vascular Endothelial Stem Cell.
Authors: Fang, Shentong, Wei, Jing, Pentinmikko, Nalle, Leinonen, Hannele, Salven, Petri
PLoS Biol, 2012-10-16;10(10):e1001407.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Extracellular matrix protein tenascin-C is required in the bone marrow microenvironment primed for hematopoietic regeneration.
Authors: Nakamura-Ishizu A, Okuno Y, Omatsu Y, Okabe K, Morimoto J, Uede T, Nagasawa T, Suda T, Kubota Y
Blood, 2012-05-02;119(23):5429-37.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Microenvironmental regulation of inducible nitric oxide synthase expression and nitric oxide production in mouse bone marrow-derived mast cells.
Authors: Moon TC, Yoshimura T, Parsons T, Befus AD
J. Leukoc. Biol., 2012-01-18;91(4):581-90.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Osteoclasts are dispensable for hematopoietic stem cell maintenance and mobilization.
Authors: Miyamoto K, Yoshida S, Kawasumi M
J. Exp. Med., 2011-10-17;208(11):2175-81.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
CCR1 expression and signal transduction by murine BMMC results in secretion of TNF-alpha, TGFbeta-1 and IL-6.
Authors: Fifadara NH, Aye CC, Raghuwanshi SK, Richardson RM, Ono SJ
Int. Immunol., 2009-07-10;21(8):991-1001.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Harboring of particulate allergens within secretory compartments by mast cells following IgE/FcepsilonRI-lipid raft-mediated phagocytosis.
Authors: Shin JS, Shelburne CP, Jin C, LeFurgey EA, Abraham SN
J. Immunol., 2006-11-01;177(9):5791-800.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
FGF2-dependent mesenchyme and laminin-111 are niche factors in salivary gland organoids
Authors: ZF Hosseini, DA Nelson, N Moskwa, LM Sfakis, J Castracane, M Larsen
J. Cell. Sci., 2018-02-20;0(0):.
-
CD11c regulates hematopoietic stem and progenitor cells under stress
Authors: Lifei Hou, Richard A. Voit, Vijay G. Sankaran, Timothy A. Springer, Koichi Yuki
Blood Advances
-
The Wilms’ tumour suppressor Wt1 is a major regulator of tumour angiogenesis and progression
Authors: Kay-Dietrich Wagner, Julien Cherfils-Vicini, Naoki Hosen, Peter Hohenstein, Eric Gilson, Nicholas D. Hastie et al.
Nature Communications
-
PDGFRa cells in mouse urinary bladder: A new class of interstitial cells.
Authors: Koh BH, Roy R, Hollywood MAet al.
J Cell Mol Med
-
Modeling mammalian spermatogonial differentiation and meiotic initiation in vitro
Authors: Oleksandr Kirsanov, Taylor Johnson, Taylor Malachowski, Bryan A. Niedenberger, Emma A. Gilbert, Debajit Bhowmick et al.
Development
-
Smchd1 is a maternal effect gene required for genomic imprinting
Authors: Iromi Wanigasuriya, Quentin Gouil, Sarah A Kinkel, Andrés Tapia del Fierro, Tamara Beck, Ellise A Roper et al.
eLife
-
Resident c-kit(+) cells in the heart are not cardiac stem cells
Authors: Nishat Sultana, Lu Zhang, Jianyun Yan, Jiqiu Chen, Weibin Cai, Shegufta Razzaque et al.
Nature Communications
-
Characterizing the Spermatogonial Response to Retinoic Acid During the Onset of Spermatogenesis and Following Synchronization in the Neonatal Mouse Testis1
Authors: Kellie S. Agrimson, Jennifer Onken, Debra Mitchell, Traci B. Topping, Hélio Chiarini-Garcia, Cathryn A. Hogarth et al.
Biology of Reproduction
-
Cerebral dopamine neurotrophic factor is essential for enteric neuronal development, maintenance, and regulation of gastrointestinal transit
Authors: Alcmène Chalazonitis, ZhiShan Li, Tuan D. Pham, Jason Chen, Meenakshi Rao, Päivi Lindholm et al.
Journal of Comparative Neurology
-
Vagal sensory innervation of the gastric sling muscle and antral wall: implications for gastro-esophageal reflux disease?
Authors: Terry L. Powley, Jared M. Gilbert, Elizabeth A. Baronowsky, Cherie N. Billingsley, Felecia N. Martin, Robert J. Phillips
Neurogastroenterology & Motility
-
Ca2+ signaling driving pacemaker activity in submucosal interstitial cells of Cajal in the murine colon
Authors: Salah A Baker, Wesley A Leigh, Guillermo Del Valle, Inigo F De Yturriaga, Sean M Ward, Caroline A Cobine et al.
eLife
-
Hypoxia Prevents Mitochondrial Dysfunction and Senescence in Human c-Kit+ Cardiac Progenitor Cells
Authors: KI Korski, DA Kubli, BJ Wang, FG Khalafalla, MM Monsanto, F Firouzi, OH Echeagaray, T Kim, RM Adamson, WP Dembitsky, ÅB Gustafsson, MA Sussman
Stem Cells, 2019-01-30;0(0):.
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Electroacupuncture at ST36 Increases Bone Marrow-Derived Interstitial Cells of Cajal via the SDF-1/CXCR4 and mSCF/Kit-ETV1 Pathways in the Stomach of Diabetic Mice
Authors: Jiao Zhao, Jing An, Shi Liu
Evidence-Based Complementary and Alternative Medicine
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Morphologic and Immunohistochemical Characterization of Spontaneous Lymphoma/Leukemia in NSG Mice
Authors: Heather Tillman, Laura J. Janke, Amy Funk, Peter Vogel, Jerold E. Rehg
Veterinary Pathology
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