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Human ErbB2/Her2 Biotinylated Antibody

Catalog #: BAF1129
13 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
BAF1129
ErbB2/Her2 in MCF-7 Human Cell Line.
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Citations (13)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ErbB2/Her2 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ErbB2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant human (rh) ErbB3, rhErbB4, and rhEGF R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ErbB2
Thr23-Thr652
Accession # P04626
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human ErbB2/Her2 Fc Chimera (Catalog # 1129-ER)
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Human ErbB2/Her2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human ErbB2/Her2 Antibody (Catalog # MAB1129)

Standard: Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF (Catalog # 1129-ER)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry ErbB2/Her2 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

ErbB2/Her2 in MCF-7 Human Cell Line. ErbB2/Her2 was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human ErbB2/Her2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1129) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry ErbB2/Her2 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

ErbB2/Her2 in Human Breast Cancer Tissue. ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human ErbB2/Her2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1129) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ErbB2/Her2

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGF R), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation (1-10).

References
  1. Coussens, L. et al. (1985) Science 230:1132.
  2. Yamamoto, T. et al. (1986) Nature 319:230.
  3. Kanai, Y. et al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
  4. Codony-Servat, J. et al. (1999) Cancer Res. 59:1196.
  5. Carraway, K.L. 3rd et al.  (1994) J. Biol. Chem. 269:14303.
  6. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  7. Schaefer, G. et al. (1999) J. Biol. Chem. 274:859.
  8. Schlessinger, J. (2000) Cell 103:211.
  9. Hellyer, N.J. et al. (2001) J. Biol. Chem. 276:42153.
  10. Daly, R.J. (1999) Growth Factors 16:255.
Long Name
Receptor Tyrosine Protein Kinase ErbB2
Entrez Gene IDs
2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)
Alternate Names
CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

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Citations for Human ErbB2/Her2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Electrochemical Cellulase-Linked ELASA for Rapid Liquid Biopsy Testing of Serum HER-2/neu
    Authors: Ana Díaz-Fernández, Alexey Ferapontov, Mikkel Holm Vendelbo, Elena E. Ferapontova
    ACS Measurement Science Au
  2. Covalent Hemin/G4 complex-linked sandwich bioassay on magnetic beads for femtomolar HER-2/neu detection in human serum via direct electrocatalytic reduction of oxygen
    Authors: A Díaz-Ferná, EE Ferapontov
    Oncogene, 2022-06-06;1219(0):340049.
    Species: N/A
    Sample Types: Beads
    Applications: Functional Assay
  3. Immuno-Acoustic Sorting of Disease-Specific Extracellular Vesicles by Acoustophoretic Force
    Authors: J Liu, Y Qu, H Wang
    Micromachines, 2021-12-09;12(12):.
    Species: Human
    Sample Types: Protein
    Applications: ELISA Capture
  4. Receptor-Driven ERK Pulses Reconfigure MAPK Signaling and Enable Persistence of Drug-Adapted BRAF-Mutant Melanoma Cells
    Authors: Luca Gerosa, Christopher Chidley, Fabian Fröhlich, Gabriela Sanchez, Sang Kyun Lim, Jeremy Muhlich et al.
    Cell Systems
  5. Femtomolar electroanalysis of a breast cancer biomarker HER-2/neu protein in human serum by the cellulase-linked sandwich assay on magnetic beads
    Authors: K Malecka, D Pankratov, EE Ferapontov
    Anal. Chim. Acta, 2019-05-25;1077(0):140-149.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture, ELISA Detection
  6. Systems Modeling Identifies Divergent Receptor Tyrosine Kinase Reprogramming to MAPK Pathway Inhibition
    Authors: Allison M. Claas, Lyla Atta, Simon Gordonov, Aaron S. Meyer, Douglas A. Lauffenburger
    Cellular and Molecular Bioengineering
  7. Multiplatform Biomarker Discovery for Bladder Cancer Recurrence Diagnosis
    Authors: Marine De Paoli, Selma Gogalic, Ursula Sauer, Claudia Preininger, Hardev Pandha, Guy Simpson et al.
    Disease Markers
  8. MAPK7 Regulates EMT Features and Modulates the Generation of CTCs
    Authors: Sarah Javaid, Jianmin Zhang, Gromoslaw A. Smolen, Min Yu, Ben S. Wittner, Anurag Singh et al.
    Molecular Cancer Research
  9. Molecular nanoshearing: an innovative approach to shear off molecules with AC-induced nanoscopic fluid flow.
    Authors: Shiddiky, Muhammad, Vaidyanathan, Ramanath, Rauf, Sakandar, Tay, Zhikai, Trau, Matt
    Sci Rep, 2014-01-16;4(0):3716.
    Species: Human
    Sample Types: Serum
    Applications: Functional Assay
  10. Ultrasensitive On-Chip Immunoassays with a Nanoparticle-Assembled Photonic Crystal
    Authors: Jin-Hee Han, L. Sudheendra, Hee-Joo Kim, Shirley J. Gee, Bruce D. Hammock, Ian M. Kennedy
    ACS Nano
  11. Microbead arrays for the analysis of ErbB receptor tyrosine kinase activation and dimerization in breast cancer cells.
    Authors: Khan IH, Zhao J, Ghosh P, Ziman M, Sweeney C, Kung HJ, Luciw PA
    Assay Drug Dev Technol, 2010-02-01;8(1):27-36.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Luminex Development, Western Blot
  12. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008-04-19;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  13. ELISA assay employing epitope-specific monoclonal antibodies to quantify circulating HER2 with potential application in monitoring cancer�patients undergoing therapy with trastuzumab
    Authors: V Agnolon, A Contato, A Meneghello, E Tagliabue, G Toffoli, M Gion, F Polo, ASC Fabricio
    Sci Rep, 2020-02-20;10(1):3016.

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Human ErbB2/Her2 Biotinylated Antibody
By Zhixin Wang on 02/03/2016
Application: Immunocytochemistry/Immunofluorescence Sample Tested: SK-BR-3 human breast cancer cell line Species: Goat
Immunocytochemistry/Immunofluorescence Human ErbB2/Her2 Biotinylated Antibody BAF1129