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Human ErbB2/Her2 Antibody

Catalog #: MAB1129
15 Citations 2 Reviews Datasheet
Catalog # Availability Size / Price Qty
MAB1129-SP
MAB1129-100
MAB1129-500
Detection of ErbB2/Her2 in MCF‑7 Human Cell Line by Flow Cytometry.
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Product Details
Citations (15)
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Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ErbB2/Her2 Antibody Summary

Species Reactivity
Human
Source
Monoclonal Mouse IgG2B Clone # 191924
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ErbB2/Her2
Thr23-Thr652
Accession # P04626
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human ErbB2/Her2 Fc Chimera (Catalog # 1129-ER)
under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Human ErbB2/Her2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human ErbB2/Her2 Biotinylated Antibody (Catalog # BAF1129)

Standard: Recombinant Human ErbB2/Her2 Fc Chimera Protein, CF (Catalog # 1129-ER)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ErbB2/Her2 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of ErbB2/Her2 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB1129, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of ErbB2/Her2 antibody in MDA-MB-453 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of ErbB2/Her2 in MDA-MB-453 Human Cell Line by Flow Cytometry. MDA-MB-453 human breast cancer cell line was stained with Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB1129, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry ErbB2/Her2 antibody in MDA-MB-231 Human Cell Line by Immunocytochemistry (ICC). View Larger

ErbB2/Her2 in MDA‑MB‑231 Human Cell Line. ErbB2/Her2 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB1129) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry ErbB2/Her2 antibody in Human Stomach by Immunohistochemistry (IHC-P). View Larger

ErbB2/Her2 in Human Stomach. ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB1129) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane/cytoplasm in glans and villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry ErbB2/Her2 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

ErbB2/Her2 in Human Breast Cancer Tissue. ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB1129) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ErbB2/Her2

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGF R), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation (1-10).

References
  1. Coussens, L. et. al. (1985) Science 230:1132.
  2. Yamamoto, T. et. al. (1986) Nature 319:230.
  3. Kanai, Y. et. al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
  4. Codony-Servat, J. et. al. (1999) Cancer Res. 59:1196.
  5. Carraway, K.L. 3rd et. al.  (1994) J. Biol. Chem. 269:14303.
  6. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  7. Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
  8. Schlessinger, J. (2000) Cell 103:211.
  9. Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
  10. Daly, R.J. (1999) Growth Factors 16:255.
Long Name
Receptor Tyrosine Protein Kinase ErbB2
Entrez Gene IDs
2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)
Alternate Names
CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

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Citations for Human ErbB2/Her2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. HER2-selective and reversible tyrosine kinase inhibitor tucatinib potentiates the activity of T-DM1 in preclinical models of HER2-positive breast cancer
    Authors: Devra Olson, Janelle Taylor, Kelsi Willis, Kelly Hensley, Sean Allred, Margo Zaval et al.
    Cancer Res Commun
  2. SERS characterization of colorectal cancer cell surface markers upon anti‐EGFR treatment
    Authors: Nana Lyu, Bernadette Pedersen, Elena Shklovskaya, Helen Rizos, Mark P. Molloy, Yuling Wang
    Exploration (Beijing)
  3. Contextual reprogramming of CAR-T cells for treatment of HER2+ cancers
    Authors: Z Yang, L Li, A Turkoz, P Chen, R Harari-Ste, M Bobbin, O Stefanson, H Choi, V Pietrobon, B Alphson, A Goswami, V Balan, A Kearney, D Patel, J Yang, D Inel, V Vinod, A Cesano, B Wang, KH Roh, LS Qi, FM Marincola
    Journal of Translational Medicine, 2021-11-07;19(1):459.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Cellphone enabled point-of-care assessment of breast tumor cytology and molecular HER2 expression from fine-needle aspirates
    Authors: Daniel Y. Joh, Jacob T. Heggestad, Shengwei Zhang, Gray R. Anderson, Jayanta Bhattacharyya, Suzanne E. Wardell et al.
    npj Breast Cancer
  5. HER2-mediated enhancement of Ebola virus entry
    Authors: M Kuroda, P Halfmann, Y Kawaoka
    PLoS Pathog, 2020-10-14;16(10):e1008900.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  6. Preclinical Activity of HER2-Selective Tyrosine Kinase Inhibitor Tucatinib as a Single Agent or in Combination with Trastuzumab or Docetaxel in Solid Tumor Models
    Authors: A Kulukian, P Lee, J Taylor, R Rosler, P de Vries, D Watson, A Forero-Tor, S Peterson
    Mol. Cancer Ther., 2020-04-01;19(4):976-987.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Antibody-drug conjugate T-DM1 treatment for HER2+ breast cancer induces ROR1 and confers resistance through activation of Hippo transcriptional coactivator YAP1
    Authors: SS Islam, M Uddin, ASM Noman, H Akter, NJ Dity, M Basiruzzma, F Uddin, J Ahsan, S Annoor, AA Alaiya, M Al-Alwan, H Yeger, WA Farhat
    EBioMedicine, 2019-05-10;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  8. Systems Modeling Identifies Divergent Receptor Tyrosine Kinase Reprogramming to MAPK Pathway Inhibition
    Authors: Allison M. Claas, Lyla Atta, Simon Gordonov, Aaron S. Meyer, Douglas A. Lauffenburger
    Cellular and Molecular Bioengineering
  9. CRISPR-Cas9 epigenome editing enables high-throughput screening for functional regulatory elements in the human genome
    Authors: TS Klann, JB Black, M Chellappan, A Safi, L Song, IB Hilton, GE Crawford, TE Reddy, CA Gersbach
    Nat. Biotechnol., 2017-04-03;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Detection of Circulating Tumour Cells in Urothelial Cancers and Clinical Correlations: Comparison of Two Methods
    Authors: E Fina, A Necchi, S Bottelli, C Reduzzi, S Pizzamigli, C Iacona, MG Daidone, P Verderio, V Cappellett
    Dis. Markers, 2017-02-22;2017(0):3414910.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. HER2 and EGFR overexpression support metastatic progression of prostate cancer to bone
    Cancer Res., 2016-10-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. High-Throughput Screening for Internalizing Antibodies by Homogeneous Fluorescence Imaging of a pH-Activated Probe
    Authors: Thilo Riedl, Egon van Boxtel, Martijn Bosch, Paul W. H. I. Parren, Arnout F. Gerritsen
    J Biomol Screen
  13. Ultrasensitive On-Chip Immunoassays with a Nanoparticle-Assembled Photonic Crystal
    Authors: Jin-Hee Han, L. Sudheendra, Hee-Joo Kim, Shirley J. Gee, Bruce D. Hammock, Ian M. Kennedy
    ACS Nano
  14. Multiplexed protein analysis using encoded antibody-conjugated microbeads
    Authors: Nora Theilacker, Eric E. Roller, Kristopher D. Barbee, Matthias Franzreb, Xiaohua Huang
    Journal of The Royal Society Interface
  15. Microbead arrays for the analysis of ErbB receptor tyrosine kinase activation and dimerization in breast cancer cells.
    Authors: Khan IH, Zhao J, Ghosh P, Ziman M, Sweeney C, Kung HJ, Luciw PA
    Assay Drug Dev Technol, 2010-02-01;8(1):27-36.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Luminex Development

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Human ErbB2/Her2 Antibody
By Adrianne Pittman on 05/19/2017
Application: Flow Sample Tested: A431 human epithelial carcinoma cell line Species: Human
Human ErbB2/Her2 Antibody
By Adrianne Pittman on 04/24/2017