Human CD28 Antibody

Catalog #: MAB11412 Datasheet

GMP Version Available: MAB11412-GMP

GMP

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB11412-100
MAB11412-SP

Recombinant Human CD28 Antibody Enhances IL-2 Secretion in Jurkat Cells.

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Human CD28 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD28 in direct ELISAs.

Source

Monoclonal Human IgG1 Clone # 29704-1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.

Applications

Recommended Concentration

Sample

T Cell Stimulation

3 µg/mL

Human PBMCs

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

T Cell Stimulation

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Recombinant Human CD28 Antibody Enhances IL-2 Secretion in Jurkat Cells. Human CD28 recombinant antibody enhances IL-2 secretion in Jurkat cells (immortalized human T cell line) treated with 5 ng/mL phorbol myristate acetate (PMA) and 0.9 µM calcium ionomycin. Antibody-mediated stimulation occurred in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA kit. The ED₅₀ for this effect is 5-50 ng/mL.

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: CD28

The Bio-Techne CD28 GMP antibody is derived from the 15e8 clone. CD28 is a critical protein expressed on the surface of T cells that provides a co-stimulatory signal necessary for T cell activation and proliferation. CD28 is structurally similar to CTLA-4, with both molecules exhibiting structural homology to the immunoglobulin (Ig) gene superfamily. CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant co-stimulatory pathways that regulate T and B cell responses. Both CD28 and CTLA-4 are composed of a single Ig V-like extracellular domain, a transmembrane domain, and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2.

Entrez Gene IDs

940 (Human); 12487 (Mouse); 102146670 (Cynomolgus Monkey)

Alternate Names

CD28 antigen (Tp44); CD28 antigen; CD28 molecule; CD28; MGC138290; T-cell-specific surface glycoprotein CD28; Tp44