Human CD28 GMP Antibody

Catalog #: MAB11412-GMP Datasheet

Recombinant Monoclonal Antibody. See GMP Antibody Protocols!

Catalog #	Availability	Size / Price	Qty
MAB11412-GMP-100

Recombinant Human CD28 GMP Antibody Enhances IL-2 Secretion in Jurkat Cells.

1 Image

All CD28 Antibodies

Product Details

Procedure

FAQs

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD28 GMP Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD28 in direct ELISAs

Source

Recombinant Monoclonal Human IgG₁ Clone # 29704-1

Purification

Protein G purified from cell culture supernatant

Immunogen

Human T lymphocytes

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Aggregation

≤10% aggregation when tested by SEC

Host Cell Protein

≤0.500 ng per μg of antibody when tested by ELISA

Host Cell DNA

≤0.0100 ng per μg of antibody when tested by PCR

Mycoplasma

Negative when tested in a ribosomal RNA hybridization assay

Applications

Recommended Concentration

Sample

T Cell Stimulation

3 µg/mL

Human PBMCs

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

T Cell Stimulation

View Larger

Recombinant Human CD28 GMP Antibody Enhances IL-2 Secretion in Jurkat Cells. Human CD28 recombinant antibody enhances IL-2 secretion in Jurkat cells (immortalized human T cell line) treated with 5 ng/mL phorbol myristate acetate (PMA) and 0.9 µM calcium ionomycin. Antibody-mediated stimulation occurred in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA kit. The ED₅₀ for this effect is 5.00-50.0 ng/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

A minimum of 12 months when stored at ≤ -20 °C, as supplied. Refer to lot specific COA for the Use by Date.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: CD28

The Bio-Techne CD28 GMP antibody is derived from the 15e8 clone. CD28 is a critical protein expressed on the surface of T cells that provides a co-stimulatory signal necessary for T cell activation and proliferation. CD28 is structurally similar to CTLA-4, with both molecules exhibiting structural homology to the immunoglobulin (Ig) gene superfamily. CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant co-stimulatory pathways that regulate T and B cell responses. Both CD28 and CTLA-4 are composed of a single Ig V-like extracellular domain, a transmembrane domain, and an intracellular domain. CD28 and CTLA-4 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2.

Entrez Gene IDs

940 (Human); 12487 (Mouse); 102146670 (Cynomolgus Monkey)

Alternate Names

CD28 antigen (Tp44); CD28 antigen; CD28 molecule; CD28; MGC138290; T-cell-specific surface glycoprotein CD28; Tp44

Product Datasheets

Product Datasheet

Manufacturing Specifications

GMP Antibodies

R&D Systems, a Bio-Techne Brand's GMP Antibodies are produced according to relevant sections of the following documents: USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products, and Eu. Ph. 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products.

R&D Systems' quality focus includes:

Manufactured and tested under an ISO 9001:2015 and ISO 13485:2016 certified quality system
Documented processes and QA control of documentation and process changes
Personnel training programs
Raw material inspection and supplier qualification/monitoring
Fully validated equipment, processes and test methods
Equipment calibration monitored and scheduled using a computerized calibration program
Facility maintenance, safety programs and pest control
Variances dispositioned using material review process
Robust product stability program

R&D Systems strives to provide our customers with the analytical characteristics of each product so that customers may determine whether our products are appropriate for their research. The Certificate of Analysis provided contains the following lot specific information:

Purity (SDS-PAGE)
Aggregation (SEC)
Endotoxin (LAL method, tested per USP<85> and Ph. Eur. 2.6.14 guidelines)
Residual Host Cell Protein and Host Cell DNA
Mycoplasma testing
Post-bottling lot-specific bioassay results (compliance with an established range) and results of microbial testing according to USP <71>

Additional testing and documentation requested by the customer can be arranged at an additional cost.

Production records and facilities are available for examination by appropriate personnel on-site at R&D Systems in Minneapolis, Minnesota USA.

R&D Systems sells GMP grade products for preclinical or clinical ex vivo use. They are not for in vivo use. Please read the following End User Terms prior to using this product.