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Human CD117/c-kit Biotinylated Antibody

Catalog #: BAF332
2 Citations Datasheet
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BAF332
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD117/c-kit Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD117/c-kit in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant mouse CD117, recombinant human (rh) PDGF R alpha, and rhPDGF R beta is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD117/c-kit
Gln26-Thr520
Accession # P10721
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CD117/c-kit (Catalog # 332-SR/CF)
Flow Cytometry
2.5 µg/106 cells
TF‑1 human erythroleukemic cell line

Human CD117/c-kit Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human CD117/c-kit Antibody (Catalog # MAB332)

Standard: Recombinant Human SCF R/c-kit (Catalog # 332-SR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD117/c-kit

CD117, the gene product of the c-kit proto-oncogen and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. The human stem cell factor receptor cDNA encodes a 972 amino acid (aa) residue precursor membrane protein with a 25 aa residue signal peptide (experimentally determined), a 495 aa residue extracellular domain, a 23 aa residue transmembrane segment and a 429 aa residue cytoplasmic domain. Stem cell factor receptor is a member of the type III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, Flt-3, PDGF, and VEGF. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. SCF binding induces receptor homodimerization and signal transduction. SCF receptor is expressed in hematopoietic progenitor cells, normal B- and T-lymphocyte progenitor cells, mast cells, germ cells, melanocytes, neurons, glial cells, placenta, kidney, lung, and gut. In addition, SCF receptor expression has also been reported in a number of human tumor cell lines. SCF receptor can be proteolytically cleaved from the cell surface and high levels of soluble SCF receptor has been detected in cell conditioned medium and human plasma. Recombinant soluble SCF receptor binds SCF with high affinity and is a potent SCF antagonist.

References
  1. Broudy, V. (1997) Blood 90:1345.
  2. Vliagoftis, H. et al. (1997) J. Allergy Clin. Immunol. 100:435.
Long Name
Stem Cell Factor Receptor
Entrez Gene IDs
3815 (Human); 16590 (Mouse); 64030 (Rat)
Alternate Names
CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFR; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

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Citations for Human CD117/c-kit Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Multiple cellular antigen detection by ICP-MS.
    Authors: Ornatsky O, Baranov VI, Bandura DR, Tanner SD, Dick J
    J. Immunol. Methods, 2005-11-21;308(1):68-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Pro-angiogenic cytokines for prediction of outcomes in patients with advanced hepatocellular carcinoma
    Authors: K Miyahara, K for the Okayama Liver Cancer Group, K Morimoto, Y Morimoto, Y Takeuchi, H Hagihara et al.
    British Journal of Cancer

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