Human CD117/c-kit APC-conjugated Antibody Summary
Gln26-Thr520
Accession # P10721
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of CD117/c-kit in TF‑1 Human Cell Line by Flow Cytometry. TF-1 human erythroleukemic cell line was stained with Mouse Anti-Human CD117/c-kit APC-conjugated Monoclonal Antibody (Catalog # FAB332A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD117/c-kit
Stem Cell Factor Receptor (CD117, the gene product of the c-kit proto-oncogene) and its ligand, Stem Cell Factor (SCF), also known as c-kit Ligand, Mast Cell Growth Factor, play essential roles in gametogenesis, melanogenesis, and hematopoiesis. The human SCF Receptor cDNA encodes a 972 amino acid (aa) residue precursor membrane protein with a 25 aa residue signal peptide (experimentally determined), a 495 aa residue extracellular domain, a 23 aa residue transmembrane segment, and a 429 aa residue cytoplasmic domain. SCF Receptor is a member of the type III subfamily of Receptor Tyrosine Kinases (RTK) that also includes the receptors for M-CSF, Flt-3, PDGF, and VEGF. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. SCF binding induces receptor homodimerization and signal transduction. SCF Receptor is expressed in hematopoietic progenitor cells, normal B and T lymphocyte progenitor cells, mast cells, germ cells, melanocytes, neurons, glial cells, placenta, kidney, lung, and gut. In addition, SCF Receptor expression has also been reported in a number of human tumor cell lines. SCF Receptor can be proteolytically cleaved from the cell surface and high levels of soluble SCF Receptor has been detected in cell conditioned medium and human plasma. Recombinant soluble SCF Receptor binds SCF with high affinity and is a potent SCF antagonist.
Product Datasheets
Citations for Human CD117/c-kit APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Natural Cardiac Extracellular Matrix Hydrogels for Cultivation of Human Stem Cell-Derived Cardiomyocytes
Authors: Donald O. Freytes, John D. O’Neill, Yi Duan-Arnold, Emily A. Wrona, Gordana Vunjak-Novakovic
Methods in Molecular Biology
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Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population.
Authors: Yang L, Soonpaa MH, Adler ED, Roepke TK, Kattman SJ, Kennedy M, Henckaerts E, Bonham K, Abbott GW, Linden RM, Field LJ, Keller GM
Nature, 2008-04-23;453(7194):524-8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
CHIR99021 combined with retinoic acid promotes the differentiation of primordial germ cells from human embryonic stem cells
Authors: Tingting Cheng, Kui Zhai, Yan Chang, Guidong Yao, Jiahuan He, Fang Wang et al.
Oncotarget
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