Human CD117/c-kit Antibody

Catalog #: AF332
17 Citations 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
AF332
AF332-SP

Cell Proliferation Induced by SCF/c‑kit Ligand and Neutralization by Human CD117/c-kit Antibody.

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All CD117/c-kit Antibodies

Product Details

Citations (17)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD117/c-kit Antibody Summary

Species Reactivity

Human

Specificity

Detects human and mouse CD117/c-kit in direct ELISAs and Western blots. In Western blots, approximately 2% cross-reactivity with recombinant human PDGF sR alpha is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD117/c-kit
Gln26-Thr520
Accession # P10721

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Simple Western

5 µg/mL

See below

Flow Cytometry

2.5 µg/10⁶ cells

TF‑1 human erythroleukemic cell line

Immunohistochemistry

5-15 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Neutralization

Measured by its ability to neutralize SCF/c‑kit Ligand-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.The Neutralization Dose (ND₅₀) is typically 0.06-0.36 μg/mL in the presence of 20 ng/mL Recombinant Human SCF/c‑kit Ligand.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by SCF/c‑kit Ligand and Neutralization by Human CD117/c-kit Antibody. Recombinant Human SCF/c-kit Ligand (Catalog # 255-SC) induces cell proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by SCF/c-kit Ligand is neutralized (green line) by increasing concentrations of Goat Anti-Human CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF332). The ND₅₀ is typically 0.06-0.36 µg/mL in the presence of 20 ng/mL of Recombinant Human SCF/c-kit Ligand.

Immunohistochemistry

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CD117/c-kit in Human Brain. CD117/c-kit was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF332) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot

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Detection of Human CD117/c-kit by Western Blot. Western blot shows lysates of MO7e human megakaryocytic leukemic cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF332) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD117/c-kit at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human CD117/c-kit by Simple Western^TM. Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit at approximately 172 kDa (as indicated) using 5 µg/mL of Goat Anti-Human CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF332) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD117/c-kit

Stem cell factor receptor (CD117, the gene product of the c-kit proto-oncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. The human stem cell factor receptor cDNA encodes a 972 amino acid (aa) residue precursor membrane protein with a 25 aa residue signal peptide (experimentally determined), a 495 aa residue extracellular domain, a 23 aa residue transmembrane segment and a 429 aa residue cytoplasmic domain. Stem cell factor receptor is a member of the type III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, Flt-3, PDGF, and VEGF. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. SCF binding induces receptor homodimerization and signal transduction. SCF receptor is expressed in hematopoietic progenitor cells, normal B- and T-lymphocyte progenitor cells, mast cells, germ cells, melanocytes, neurons, glial cells, placenta, kidney, lung, and gut. In addition, SCF receptor expression has also been reported in a number of human tumor cell lines. SCF receptor can be proteolytically cleaved from the cell surface and high levels of soluble SCF receptor has been detected in cell conditioned medium and human plasma. Recombinant soluble SCF receptor binds SCF with high affinity and is a potent SCF antagonist.

References

Broudy, V. (1997) Blood 90:1345.
Vliagoftis, H. et al. (1997) J. Allergy Clin. Immunol. 100:435.

Long Name

Stem Cell Factor Receptor

Entrez Gene IDs

3815 (Human); 16590 (Mouse); 64030 (Rat)

Alternate Names

CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFR; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Human CD117/c-kit Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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N-Cadherin is a prospective cell surface marker of human mesenchymal stem cells that have high ability for cardiomyocyte differentiation
Authors: Hisako Ishimine, Norio Yamakawa, Mari Sasao, Mika Tadokoro, Daisuke Kami, Shinji Komazaki et al.
Biochemical and Biophysical Research Communications
Selective immunocapture reveals neoplastic human mast cells secrete distinct microvesicle- and exosome-like populations of KIT-containing extracellular vesicles
Authors: Pfeiffer A, Petersen JD, Falduto GH et al.
Journal of extracellular vesicles
Oscillating calcium signals in smooth muscle cells underlie the persistent basal tone of internal anal sphincter
Authors: Ping Lu, Jun Chen, Chenghai Zhang, Dieter Saur, Christina E. Baer, Lawrence M. Lifshitz et al.
Journal of Cellular Physiology
Single-Cell RNA Sequencing of Human, Macaque, and Mouse Testes Uncovers Conserved and Divergent Features of Mammalian Spermatogenesis
Authors: Adrienne Niederriter Shami, Xianing Zheng, Sarah K. Munyoki, Qianyi Ma, Gabriel L. Manske, Christopher D. Green et al.
Developmental Cell
Fluorescence- and magnetic-activated cell sorting strategies to isolate and enrich human spermatogonial stem cells
Authors: Hanna Valli, Meena Sukhwani, Serena L. Dovey, Karen A. Peters, Julia Donohue, Carlos A. Castro et al.
Fertility and Sterility
In vitro quantification of pigment production and transfer in 2D co-cultures and 3D skin organotypic
Authors: Rachel Phua, Tracy Zhijun Tien, Gracia Goh, Nikolaos Pantelireis, Kenice Siew Hoon Sim, Jamien Lim et al.
STAR Protocols
Use of Patterned Collagen Coated Slides to Study Normal and Scleroderma Lung Fibroblast Migration
Authors: Bahja Ahmed Abdi, Henry Lopez, Sarah Karrar, Elisabetta Renzoni, Athol Wells, Angela Tam et al.
Scientific Reports
The effects of restricted glycolysis on stem-cell like characteristics of breast cancer cells
Authors: Arindam Banerjee, Pardis Arvinrad, Matthew Darley, Stéphanie A. Laversin, Rachel Parker, Matthew J.J. Rose-Zerilli et al.
Oncotarget
CARF promotes spermatogonial self-renewal and proliferation through Wnt signaling pathway
Authors: W Cui, X He, X Zhai, H Zhang, Y Zhang, F Jin, X Song, D Wu, Q Shi, L Li
Cell Discovery, 2020-11-17;6(1):85.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
The Dynamic Transcriptional Cell Atlas of Testis Development during Human Puberty
Authors: J Guo, X Nie, M Giebler, H Mlcochova, Y Wang, EJ Grow, R Kim, M Tharmaling, G Matilionyt, C Lindskog, DT Carrell, RT Mitchell, A Goriely, JM Hotaling, BR Cairns
Cell Stem Cell, 2020-01-09;26(2):262-276.e4.
Species: Human
Sample Types: Whole Tissue
Applications: IHC

PLZFposc-KITpos-delineated A1-A4-differentiating spermatogonia by subset and stage detection upon Bouin fixation
Authors: RL Tang, LQ Fan
Asian J. Androl., 2019-05-01;21(3):309-318.
Species: Mouse, Mouseq
Sample Types: Whole Tissue
Applications: IHC, IHC-P

Regulation of human skin pigmentation in situ by repetitive UV exposure: molecular characterization of responses to UVA and/or UVB.
Authors: Choi W, Miyamura Y, Wolber R, Smuda C, Reinhold W, Liu H, Kolbe L, Hearing VJ
J. Invest. Dermatol., 2010-02-11;130(6):1685-96.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Chemotherapy-induced thrombocytopenia derives from the selective death of megakaryocyte progenitors and can be rescued by stem cell factor.
Authors: Zeuner A, Signore M, Martinetti D, Bartucci M, Peschle C, De Maria R
Cancer Res., 2007-05-15;67(10):4767-73.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Granulocyte colony-stimulating factor induces the release in the bone marrow of proteases that cleave c-KIT receptor (CD117) from the surface of hematopoietic progenitor cells.
Authors: Levesque JP, Hendy J, Winkler IG, Takamatsu Y, Simmons PJ
Exp. Hematol., 2003-02-01;31(2):109-17.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot

Motesanib inhibits Kit mutations associated with gastrointestinal stromal tumors
Authors: Sean Caenepeel, Lisa Renshaw-Gegg, Angelo Baher, Tammy L Bush, Will Baron, Todd Juan et al.
Journal of Experimental & Clinical Cancer Research

Gender-Related Differences of Tachykinin NK2 Receptor Expression and Activity in Human Colonic Smooth Muscle
Authors: Stelina Drimousis, Irit Markus, Tim V. Murphy, D. Shevy Perera, Kim-Chi Phan-Thien, Li Zhang et al.
Journal of Pharmacology and Experimental Therapeutics

FOXP4 promotes proliferation of human spermatogonial stem cells
Authors: Shu-Wei Luo, Le Tang, Dai Zhou, Hao Bo, Li-Qing Fan
Asian Journal of Andrology