Human CD117/c-kit Alexa Fluor® 488-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: CD117/c-kit

Stem cell factor receptor (CD117, the gene product of the c-kit proto-oncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. The human stem cell factor receptor cDNA encodes a 972 amino acid (aa) residue precursor membrane protein with a 25 aa residue signal peptide (experimentally determined), a 495 aa residue extracellular domain, a 23 aa residue transmembrane segment and a 429 aa residue cytoplasmic domain. Stem cell factor receptor is a member of the type III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, Flt-3, PDGF, and VEGF. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. SCF binding induces receptor homodimerization and signal transduction. SCF receptor is expressed in hematopoietic progenitor cells, normal B- and T-lymphocyte progenitor cells, mast cells, germ cells, melanocytes, neurons, glial cells, placenta, kidney, lung, and gut. In addition, SCF receptor expression has also been reported in a number of human tumor cell lines. SCF receptor can be proteolytically cleaved from the cell surface and high levels of soluble SCF receptor has been detected in cell conditioned medium and human plasma. Recombinant soluble SCF receptor binds SCF with high affinity and is a potent SCF antagonist.

Product Datasheets

Related Research Areas

• Acute Myeloid Leukemia
• Airway Inflammation and Remodeling
• B Cell Markers
• Cancer Biomarkers
• Cancer Stem Cell Markers
• Cardiac Stem Cell Markers
• Embryonic and Induced Pluripotent Stem Cell Markers
• Endothelial Cell Development Molecules
• Endothelial Cell Differentiation Markers
• Endothelial Progenitor and Endothelial Cell Markers
• Endothelial Progenitor Cell Markers
• Gametes and Fertilization
• Group 1 ILCs
• Group 2 ILCs
• Hematopoietic Progenitor Cell Markers
• Hematopoietic Stem Cell Markers
• LTi Cells
• MDSC Phenotyping - Negative Markers
• Mesenchymal Stem Cell Markers
• Microglia Development
• Myeloid Lineage Markers
• NCR+ILC3
• NCR-ILC3
• Primordial and Mature Germ Cells
• Receptor Tyrosine Kinases (RTKs) in the Akt Pathway
• Receptors in the Jak/STAT Pathway
• SCF, Flt-3 Ligand and M-CSF