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Human Axl Antibody

Catalog #: MAB154
31 Citations 2 Reviews Datasheet
Catalog # Availability Size / Price Qty
MAB154-SP
MAB154-100
MAB154-500
Detection of Axl in HeLa Human Cell Line by Flow Cytometry.
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Product Details
Citations (31)
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Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Axl Antibody Summary

Species Reactivity
Human
Specificity
Detects human Axl in direct ELISAs. In direct ELISAs, this antibody shows no cross-reactivity with recombinant mouse Axl, recombinant human (rh) Dtk or rhMer.
Source
Monoclonal Mouse IgG1 Clone # 108724
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Axl
Met1-Pro440
Accession # AAA61243
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Knockout Validated
Axl is specifically detected in A431 human epithelial carcinoma parental cell line but is not detectable in Axl knockout A431 cell line.
 

Human Axl Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Axl Biotinylated Antibody (Catalog # BAF154)

Standard: Recombinant Human Axl Fc Chimera Protein, CF (Catalog # 154-AL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Axl antibody in HeLa Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Axl in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Axl Monoclonal Antibody (Catalog # MAB154, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Axl antibody in A431 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Axl in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Axl Monoclonal Antibody (Catalog # MAB154, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Knockout Validated Axl Antibody Specificity is Shown by Flow Cytometry in Knockout Cell Line. View Larger

Axl Specificity is Shown by Flow Cytometry in Knockout Cell Line. Axl knockout A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Axl Monoclonal Antibody (Catalog # MAB154, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). No staining in the Axl knockout A431 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Human Human Axl Antibody by Flow Cytometry View Larger

Detection of Human Human Axl Antibody by Flow Cytometry MR766 infection of unstimulated and decidualized T-HESC cell line.(a) T-HESC (left) and decidualized (d) T-HESC (right) were stained with ZIKV anti-dsRNA or E protein mAbs whereas the nuclei were stained with Hoechst. (b) Surface expression of AXL (red) and MER (blue) in T-HESC (left) and dT-HESC (right) was determined in uninfected cells by flow cytometry. The histograms of one experiment representative of 3 independently performed are shown. Double immunostaining for ZIKV E protein and calreticulin (c) or dsRNA and vimentin (d) in dT-HESC either uninfected or infected with MR766 at 72 h post-infection; Hoechst was used to stain nuclei. Arrows indicate localization of E protein in areas occupied by calreticulin. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28281680), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

References
  1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
  2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  3. Holland, S. et al. (2005) Canc. Res. 65:9294.
Long Name
Axl Receptor Tyrosine Kinase
Entrez Gene IDs
558 (Human); 26362 (Mouse); 102126361 (Cynomolgus Monkey)
Alternate Names
AI323647; ARK; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; Axl; EC 2.7.10; EC 2.7.10.1; JTK11; Tyro7; tyrosine-protein kinase receptor UFO; UFO; UFOoncogene AXL

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Citations for Human Axl Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. Complex Autoinflammatory Syndrome Unveils Fundamental Principles of JAK1 Kinase Transcriptional and Biochemical Function
    Authors: Conor N. Gruber, Jorg J.A. Calis, Sofija Buta, Gilad Evrony, Jerome C. Martin, Skyler A. Uhl et al.
    Immunity
  2. Differential IRF8 Transcription Factor Requirement Defines Two Pathways of Dendritic Cell Development in Humans
    Authors: Urszula Cytlak, Anastasia Resteu, Sarah Pagan, Kile Green, Paul Milne, Sheetal Maisuria et al.
    Immunity
  3. Axl receptor tyrosine kinase expression in breast cancer
    Authors: Timothy M D'Alfonso, Jeffrey Hannah, Zhengming Chen, Yifang Liu, Pengbo Zhou, Sandra J Shin
    Journal of Clinical Pathology
  4. The AXL Receptor Is a Sensor of Ligand Spatial Heterogeneity
    Authors: Aaron S. Meyer, Annelien J.M. Zweemer, Douglas A. Lauffenburger
    Cell Systems
  5. AXL Targeting Abrogates Autophagic Flux and Induces Immunogenic Cell Death in Drug-Resistant Cancer Cells
    Authors: Maria L. Lotsberg, Katarzyna Wnuk-Lipinska, Stéphane Terry, Tuan Zea Tan, Ning Lu, Laura Trachsel-Moncho et al.
    Journal of Thoracic Oncology
  6. The First-In-Class Anti-AXL×CD3epsilon Pronectin™-Based Bispecific T-Cell Engager Is Active in Preclinical Models of Human Soft Tissue and Bone Sarcomas
    Authors: N Polerà, A Mancuso, C Riillo, D Caracciolo, S Signorelli, K Grillone, S Ascrizzi, CA Hokanson, F Conforti, N Staropoli, L Gervasi, MT Di Martino, M Arbitrio, G Nisticò, R Crea, P Tagliaferr, G Juli, P Tassone
    Cancers, 2023-03-08;15(6):.
  7. Vimentin regulates EMT induction by Slug and oncogenic H-Ras and migration by governing Axl expression in breast cancer
    Authors: K Vuoriluoto, H Haugen, S Kiviluoto, J-P Mpindi, J Nevo, C Gjerdrum et al.
    Oncogene
  8. Human Endometrial Stromal Cells Are Highly Permissive To Productive Infection by Zika Virus
    Authors: Isabel Pagani, Silvia Ghezzi, Adele Ulisse, Alicia Rubio, Filippo Turrini, Elisabetta Garavaglia et al.
    Scientific Reports
  9. Dissecting signaling regulators driving AXL-mediated bypass resistance and associated phenotypes by phosphosite perturbations
    Authors: Creixell, M;Taylor, SD;Gerritsen, J;Bae, SY;Jiang, M;Augustin, T;Loui, M;Boixo, C;Creixell, P;White, FM;Meyer, AS;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA Capture
  10. Transitional dendritic cells are distinct from conventional DC2 precursors and mediate proinflammatory antiviral responses
    Authors: Sulczewski, FB;Maqueda-Alfaro, RA;Alcántara-Hernández, M;Perez, OA;Saravanan, S;Yun, TJ;Seong, D;Arroyo Hornero, R;Raquer-McKay, HM;Esteva, E;Lanzar, ZR;Leylek, RA;Adams, NM;Das, A;Rahman, AH;Gottfried-Blackmore, A;Reizis, B;Idoyaga, J;
    Nature immunology
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Single cell analysis in head and neck cancer reveals potential immune evasion mechanisms during early metastasis
    Authors: HS Quah, EY Cao, L Suteja, CH Li, HS Leong, FT Chong, S Gupta, C Arcinas, JF Ouyang, V Ang, T Celhar, Y Zhao, HC Tay, J Chan, T Takahashi, DSW Tan, SK Biswas, OJL Rackham, NG Iyer
    Nature Communications, 2023-03-27;14(1):1680.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity
    Authors: R Leylek, M Alcántara-, JM Granja, M Chavez, K Perez, OR Diaz, R Li, AT Satpathy, HY Chang, J Idoyaga
    Cell Rep, 2020-09-22;32(12):108180.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Characterization of zika virus infection of human fetal cardiac mesenchymal stromal cells
    Authors: F Rossi, B Josey, EC Sayitoglu, R Potens, T Sultu, AD Duru, V Beljanski
    PLoS ONE, 2020-09-17;15(9):e0239238.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Systemic Type I IFN Inflammation in Human ISG15 Deficiency Leads to Necrotizing Skin Lesions
    Authors: M Martin-Fer, M Bravo Garc, C Gruber, S Murias Loz, MNH Malik, F Alsohime, A Alakeel, R Valdez, S Buta, G Buda, MA Marti, M Larralde, B Boisson, M Feito Rodr, X Qiu, M Chrabieh, M Al Ayed, S Al Muhsen, JV Desai, EMN Ferre, SD Rosenzweig, B Amador-Bor, LY Bravo-Gall, R Olmer, S Merkert, M Bret, AK Sood, A Al-Rabiaah, MH Temsah, R Halwani, M Hernandez, F Pessler, JL Casanova, J Bustamante, MS Lionakis, D Bogunovic
    Cell Rep, 2020-05-12;31(6):107633.
    Species: Human
    Sample Types:
    Applications: CyTof
  15. Comprehensive Immunoprofiling of Pediatric Zika Reveals Key Role for Monocytes in the Acute Phase and No Effect of Prior Dengue Virus Infection
    Authors: D Michlmayr, EY Kim, AH Rahman, R Raghunatha, S Kim-Schulz, Y Che, S Kalayci, ZH Gümü?, G Kuan, A Balmaseda, A Kasarskis, SM Wolinsky, M Suaréz-Far, E Harris
    Cell Rep, 2020-04-28;31(4):107569.
    Species: Human
    Sample Types: Plasma
    Applications: CyTof
  16. MERTK acts as a costimulatory receptor on human CD8+ T cells
    Authors: MJW Peeters, D Dulkeviciu, A Draghi, C Ritter, A Rahbech, SK Skadborg, T Seremet, AM Carnaz Sim, E Martinenai, HR Halldórsdó, MH Andersen, G Holmen Olo, IM Svane, LJ Rasmussen, O Met, JC Becker, M Donia, C Desler, P Thor Strat
    Cancer Immunol Res, 2019-07-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection.
    Authors: Cerny D, Haniffa M, Shin A, Bigliardi P, Tan B, Lee B, Poidinger M, Tan E, Ginhoux F, Fink K
    PLoS Pathog, 2014-12-04;10(12):e1004548.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Flow cytometry-based functional selection of RNA interference triggers for efficient epi-allelic analysis of therapeutic targets.
    Authors: Micklem D, Blo M, Bergstrom P, Hodneland E, Tiron C, Hoiby T, Gjerdrum C, Hammarsten O, Lorens J
    BMC Biotechnol, 2014-06-21;14(0):57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Circulating levels of soluble MER in lupus reflect M2c activation of monocytes/macrophages, autoantibody specificities and disease activity.
    Authors: Zizzo G, Guerrieri J, Dittman L, Merrill J, Cohen P
    Arthritis Res Ther, 2013-01-01;15(6):R212.
    Species: Human
    Sample Types: Plasma
  20. Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis.
    J. Exp. Med., 2012-10-15;209(11):2033-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry.
    Authors: Shimojima M, Stroher U, Ebihara H, Feldmann H, Kawaoka Y
    J. Virol., 2011-12-07;86(4):2067-78.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor.
    Authors: Zhu D, Wang Y, Singh I
    Blood, 2010-03-26;115(23):4963-72.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Survival and migration of human dendritic cells are regulated by an IFN-alpha-inducible Axl/Gas6 pathway.
    Authors: Scutera S, Fraone T, Musso T, Cappello P, Rossi S, Pierobon D, Orinska Z, Paus R, Bulfone-Paus S, Giovarelli M
    J. Immunol., 2009-08-05;183(5):3004-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Selection
  24. Up-regulation of soluble Axl and Mer receptor tyrosine kinases negatively correlates with Gas6 in established multiple sclerosis lesions.
    Authors: Weinger JG, Omari KM, Marsden K, Raine CS, Shafit-Zagardo B
    Am. J. Pathol., 2009-06-18;175(1):283-93.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  25. Multiple roles for the receptor tyrosine kinase axl in tumor formation.
    Authors: Holland SJ, Powell MJ, Franci C, Chan EW, Friera AM, Atchison RE, McLaughlin J, Swift SE, Pali ES, Yam G, Wong S, Lasaga J, Shen MR, Yu S, Xu W, Hitoshi Y, Bogenberger J, Nör JE, Payan DG, Lorens JB
    Cancer Res., 2005-10-15;65(20):9294-303.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  26. A Potent and Selective Dual Inhibitor of AXL and MERTK Possesses Both Immunomodulatory and Tumor-Targeted Activity
    Authors: Jonathan Rios-Doria, Margaret Favata, Kerri Lasky, Patricia Feldman, Yvonne Lo, Gengjie Yang et al.
    Frontiers in Oncology
  27. Targeting of the AXL receptor tyrosine kinase by small molecule inhibitor leads to AXL cell surface accumulation by impairing the ubiquitin-dependent receptor degradation
    Authors: Markus Lauter, Anja Weber, Robert Torka
    Cell Communication and Signaling
  28. Systems Modeling Identifies Divergent Receptor Tyrosine Kinase Reprogramming to MAPK Pathway Inhibition
    Authors: Allison M. Claas, Lyla Atta, Simon Gordonov, Aaron S. Meyer, Douglas A. Lauffenburger
    Cellular and Molecular Bioengineering
  29. Apoptotic Bodies Elicit Gas6-Mediated Migration of AXL-Expressing Tumor Cells
    Authors: Annelien J.M. Zweemer, Cory B. French, Joshua Mesfin, Simon Gordonov, Aaron S. Meyer, Douglas A. Lauffenburger
    Molecular Cancer Research
  30. Quantitative Proteomics Analysis of Lytic KSHV Infection in Human Endothelial Cells Reveals Targets of Viral Immune Modulation
    Authors: Ildar Gabaev, James C. Williamson, Thomas W.M. Crozier, Thomas F. Schulz, Paul J. Lehner
    Cell Reports
  31. Harnessing MerTK agonism for targeted therapeutics
    Authors: Vivekananda Kedage, Diego Ellerman, Yongmei Chen, Wei-Ching Liang, Joven Borneo, Yan Wu et al.
    mAbs

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Human Axl Antibody
By Anonymous on 07/31/2020
Application: ELISA Sample Tested: Serum Species: Human
ELISA Human Axl Antibody MAB154
Human Axl Antibody
By Anonymous on 06/07/2016
Application: Func Sample Tested: NCI-H1299 Species: Human
Functional Assay Human Axl Antibody MAB154