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Recombinant Human Axl Fc Chimera Protein, CF

Catalog #: 154-AL
12 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
154-AL-100
R&D Systems Recombinant Proteins and Enzymes
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Citations (12)
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Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Axl Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Axl Fc Chimera is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Human Gas6 (Catalog # 885-GS) that produces 50% of the optimal binding response is approximately 2.5‑15 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human Axl protein
Human Axl
(Glu33-Pro440)
Accession # AAA61243		 DIEGRMD Human IgG1
(Pro100-Lys330) 		6-His tag
N-terminus C-terminus
Accession #
AAA61243
N-terminal Sequence
Analysis
Glu33
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
71.7 kDa (monomer)
SDS-PAGE
100-110 kDa, reducing conditions

Product Datasheets

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154-AL

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

154-AL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Axl

Axl, also known as Ufo and Ark, is a widely expressed 140 kDa glycoprotein in the TAM receptor tyrosine kinase family. TAM family receptors (Dtk/Tyro3, Axl, and Mer) are involved in regulation of the inflammatory response, cell survival and migration, and tumorigenesis (1). Mature human Axl consists of a 426 aa extracellular domain (ECD) that contains two Ig-like domains and two fibronectin type III domains, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain that includes the tyrosine kinase domain (2). Within the ECD, human Axl shares approximately 82% aa sequence identity with mouse and rat Axl. A short alternately spliced form of human Axl has a 9 aa deletion in the extracellular juxtamembrane region (2). Axl binds the vitamin K-dependent protein Gas6 which triggers tyrosine autophosphorylation of the Axl cytoplasmic domain (3). Activation of Axl induces a broad range of activities including platelet aggregation and thrombus formation (4), macrophage and dendritic cell phagocytosis of apoptotic cells (5), NK cell development from hematopoietic progenitor cells (6), and in vivo angiogenesis (7). Axl is highly expressed in solid cancers and promotes in vivo tumorigenesis and tumor cell invasiveness (7, 8). It contributes to vascular remodeling and inflammatory cell infiltration in response to hypertension and restricted blood flow (9). It also functions as a cellular entry receptor for Gas6-opsonized lentiviruses (10). A 70-80 kDa soluble portion of the Axl ECD can be shed by proteolytic cleavage, and this fragment retains the ability to bind Gas6 (11, 12).

References
  1. Linger, R.M.A. et al. (2011) Adv. Cancer Res. 100:35.
  2. O’Bryan, J.P. et al. (1991) Mol. Cell. Biol. 11:5016.
  3. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  4. Cosemans, J.M.E.M. et al. (2010) J. Thromb. Haemost. 8:1797.
  5. Seitz, H.M. et al. (2007) J. Immunol. 178:5635.
  6. Park, I.-K. et al. (2009) Blood 113:2470.
  7. Holland, S. et al. (2005) Cancer Res. 65:9294.
  8. Rankin, E.B. et al. (2010) Cancer Res. 70:7570.
  9. Korshunov, V.A. et al. (2006) Circ. Res. 98:1446.
  10. Morizono, K. et al. (2011) Cell Host Microbe 9:286.
  11. O’Bryan, J.P. et al. (1995) J. Biol. Chem. 270:551.
  12. Ekman, C. et al. (2010) J. Thromb. Haemost. 8:838.
Long Name
Axl Receptor Tyrosine Kinase
Entrez Gene IDs
558 (Human); 26362 (Mouse); 102126361 (Cynomolgus Monkey)
Alternate Names
AI323647; ARK; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; Axl; EC 2.7.10; EC 2.7.10.1; JTK11; Tyro7; tyrosine-protein kinase receptor UFO; UFO; UFOoncogene AXL

Citations for Recombinant Human Axl Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. AXL-Receptor Targeted 14FN3 Based Single Domain Proteins (Pronectins�) from 3 Synthetic Human Libraries as Components for Exploring Novel Bispecific Constructs against Solid Tumors
    Authors: CA Hokanson, E Zacco, G Cappuccill, T Odineca, R Crea
    Biomedicines, 2022-12-08;10(12):.
    Species: Mouse
    Sample Types: Whole Cell
    Applications: Bioassay
  2. Ligand-dependent kinase activity of MERTK drives efferocytosis in human iPSC-derived macrophages
    Authors: F Wanke, S Gutbier, A Rümmelin, M Steinberg, LD Hughes, M Koenen, J Komuczki, D Regan-Komi, S Wagage, J Hesselmann, R Thoma, D Brugger, T Christopei, H Wang, F Point, R Hallet, S Ghosh, CV Rothlin, C Patsch, B Geering
    Cell Death & Disease, 2021-05-25;12(6):538.
    Species: Human
    Sample Types: Protein
    Applications: SPR
  3. In vivo microscopy reveals macrophage polarization locally promotes coherent microtubule dynamics in migrating cancer cells
    Authors: G Luthria, R Li, S Wang, M Prytyskach, RH Kohler, DA Lauffenbur, TJ Mitchison, R Weissleder, MA Miller
    Nat Commun, 2020-07-14;11(1):3521.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Efficient blockade of locally reciprocated tumor-macrophage signaling using a TAM-avid nanotherapy
    Authors: SJ Wang, R Li, TSC Ng, G Luthria, MJ Oudin, M Prytyskach, RH Kohler, R Weissleder, DA Lauffenbur, MA Miller
    Sci Adv, 2020-05-22;6(21):eaaz8521.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Hematopoietic niche drives FLT3-ITD acute myeloid leukemia resistance to quizartinib via STAT5- and hypoxia- dependent up-regulation of AXL
    Authors: PY Dumas, C Naudin, S Martin-Lan, B Izac, L Casetti, O Mansier, B Rousseau, A Artus, M Dufossée, A Giese, P Dubus, A Pigneux, V Praloran, A Bidet, A Villacrece, AV Guitart, N Milpied, O Kosmider, I Vigon, V Desplat, I Dusanter-F, JM Pasquet
    Haematologica, 2019-03-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Engineering Axl specific CAR and SynNotch receptor for cancer therapy.
    Authors: Cho J, Okuma A, Al-Rubaye D, Intisar E, Junghans R, Wong W
    Sci Rep, 2018-03-01;8(1):3846.
    Species: Human
    Sample Types: Whole Cells
    Applications: CAR-T
  7. Receptor tyrosine kinase Axl is required for resistance of leukemic cells to FLT3-targeted therapy in acute myeloid leukemia.
    Authors: Park I, Mundy-Bosse B, Whitman S, Zhang X, Warner S, Bearss D, Blum W, Marcucci G, Caligiuri M
    Leukemia, 2015-06-19;29(12):2382-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Circulating levels of soluble MER in lupus reflect M2c activation of monocytes/macrophages, autoantibody specificities and disease activity.
    Authors: Zizzo G, Guerrieri J, Dittman L, Merrill J, Cohen P
    Arthritis Res Ther, 2013-01-01;15(6):R212.
    Species: Human
    Sample Types: Plasma
  9. Targeting Axl with an high-affinity inhibitory aptamer.
    Authors: Cerchia, Laura, Esposito, Carla L, Camorani, Simona, Rienzo, Anna, Stasio, Loredana, Insabato, Luigi, Affuso, Andrea, de Franciscis, Vittorio
    Mol Ther, 2012-08-21;20(12):2291-303.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Axl is essential for VEGF-A-dependent activation of PI3K/Akt.
    Authors: Ruan GX, Kazlauskas A
    EMBO J., 2012-02-10;31(7):1692-703.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry.
    Authors: Shimojima M, Stroher U, Ebihara H, Feldmann H, Kawaoka Y
    J. Virol., 2011-12-07;86(4):2067-78.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay
  12. Human cutaneous melanomas lacking MITF and melanocyte differentiation antigens express a functional Axl receptor kinase.
    Authors: Sensi M, Catani M, Castellano G
    J. Invest. Dermatol., 2011-07-28;131(12):2448-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human Axl Fc Chimera Protein, CF
By Anonymous on 06/07/2017
Application: In vivo study