HBC 530
Chemical Name: 4-Cyano-α-[[4-[(2-hydroxyethyl)methylamino]phenyl]methylene]benzeneacetonitrile
Purity: ≥98%
Biological Activity
HBC 530 is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in live cells. Fluoresces when bound to Pepper aptamer; displays high affinity binding (Kd = 3.5 nM). Suitable for confocal and two-photon microscopy. Confocal excitation maximum = 488 nm, emission maximum = 495 - 595 nm. Two-photon excitation maximum = 976 nm, emission maximum = 475 - 575 nm.To make a 1000x stock solution, add 200 μL DMSO (Cat. No. 3176).
Optical Data for HBC 530
| λabs | 485 nm |
|---|---|
| λem | 530 nm |
| Extinction Coefficient (ε) | 65300 M-1cm-1 |
| Quantum Yield (φ) | 0.66 |
| Closest Laser line | 488 nm |
| Application | Fluorogen, used with RNA Light-up Aptamer, Confocal Microscopy |
| Alternative For | Cat. No. 6434 |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs.
Chen et al.
Nat.Biotechnol., 2019;37:1287
Product Datasheets
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