8-Bromo-cAMP, sodium salt

Biological Activity

8-Bromo-cAMP, sodium salt is a cell-permeable cyclic AMP (cAMP) analog. It activates PKA and has greater resistance to hydrolysis by phosphodiesterase (PDE) than cAMP. It increases differentiation and induces apoptosis in an esophageal cancer cell line. 8-Bromo-cAMP enhances the induction of pluripotency in human fibroblast cells in combination with VPA (valproic acid) (Cat. No. 2815). In combination with IBMX (Cat. No. 2845), it promotes the differentiation of human iPS cell-derived intestinal epithelial cells. It induces cell-based VEGF production for angiogenesis and osteoblastic differentiation in vitro.

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. Human mesenchymal stem/stromal cells cultured as spheroids are self-activated to produce prostaglandin E2 that directs stimulated macrophages into an anti-inflammatory phenotype.
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   Stem Cells, 2012;30(10):2283-96.
2. Protein kinase A activation inhibits DUX4 gene expression in myotubes from patients with facioscapulohumeral muscular dystrophy
   JM Cruz, N Hupper, LS Wilson, JB Concannon, Y Wang, B Oberhauser, K Patora-Kom, Y Zhang, DJ Glass, AU Trendelenb, BA Clarke
   J. Biol. Chem., 2018;0(0):.
3. The role of mislocalized phototransduction in photoreceptor cell death of retinitis pigmentosa.
   Nakao T, Tsujikawa M, Notomi S
   PLoS ONE, 2012;7(4):e32472.
4. Sustained signalling by PTH modulates IP3 accumulation and IP3 receptors through cyclic AMP junctions.
   Meena A, Tovey S, Taylor C
   J Cell Sci, 2015;128(2):408-20.
5. Functional vascularized lung grafts for lung bioengineering
   NV Dorrello, BA Guenthart, JD O'Neill, J Kim, K Cunningham, YW Chen, M Biscotti, T Swayne, HM Wobma, SXL Huang, HW Snoeck, M Bacchetta, G Vunjak-Nov
   Sci Adv, 2017;3(8):e1700521.
6. High-throughput genotoxicity assay identifies antioxidants as inducers of DNA damage response and cell death.
   Fox JT, Sakamuru S, Huang R
   Proc. Natl. Acad. Sci. U.S.A., 2012;109(14):5423-8.
7. Phosphorylation of mouse melanopsin by protein kinase a.
   Blasic, Joseph R, Brown, R Lane, Robinson, Phyllis
   PLoS ONE, 2012;7(9):e45387.
8. Distinct terminal and cell body mechanisms in the nociceptor mediate hyperalgesic priming.
   Ferrari L, Araldi D, Levine J
   J Neurosci, 2015;35(15):6107-16.
9. A cyclic AMP analog, 8-Br-cAMP, enhances the induction of pluripotency in human fibroblast cells.
   Wang et al.
   Stem Cell Rev., 2011;7:331
10. Protein kinase A induces recruitment of active Na⁺,K⁺-ATPase units to the plasma membrane of rat proximal convoluted tubule cells.
    Carranza et al.
    J.Physiol., 1998;511:235
11. Functional modulation of P2X₂ receptors by cyclic AMP-dependent protein kinase.
    Chow et al.
    J.Neurochem., 1998;70:2606
12. Lack of correlation between activation of cyclic AMP-dependent protein kinase and inhibition of contraction of rat vas deferens by cyclic AMP analogs
    Hei et al.
    Mol.Pharmacol., 1991;39:233
13. Lack of correlation between activation of cyclic AMP-dependent protein kinase and inhibition of contraction of rat vas deferens by cyclic AMP analogs.
    Hei et al.
    Mol.Pharmacol., 1991;39:233
14. Dual effects of 8-Br-cAMP on differentiation and apoptosis of human esophageal cancer cell line Eca-109.
    Wang et al.
    World J.Gastroenterol., 2005;11:6538
15. One-day treatment of small molecule 8-bromo-cyclic AMP analogue induces cell-based VEGF production for in vitro angiogenesis and osteoblastic differentiation.
    Lo et al.
    J.Tissue.Eng.Regen. Med., 2016;10:867

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