Human/Mouse Bax Minus C-Terminus Antibody

Catalog #: MAB883 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB883
MAB883-SP

Detection of Human/Mouse Bax Minus C-Terminus by Western Blot.

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Human/Mouse Bax Minus C-Terminus Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse full-length Bax in Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 127602

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Bax minus the carboxyl terminal

Formulation

Supplied as a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1.0-2.0 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse Bax Minus C-Terminus by Western Blot. Western blot shows lysates of L-929 mouse fibroblast cell line and THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1.0-2.0 µg/mL of Human/Mouse Bax Minus C-Terminus Monoclonal Antibody (Catalog # MAB883) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Bax Minus C-Terminus at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Immunocytochemistry

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Bax in A549 Human Cell Line. Bax was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human/Mouse Bax Minus C-Terminus Monoclonal Antibody (Catalog # MAB883) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry

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Bax in RAW264.7 cells. Bax was detected in immersion fixed RAW264.7 cells using Mouse Anti-Human/Mouse Bax Minus C-Terminus Monoclonal Antibody (Catalog # MAB883) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Preparation and Storage

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.

Background: Bax

Bax belongs to the Bcl2 protein family. It forms homodimers and heterodimers with Bcl2 and functions as an apoptotic activator. Bax interacts with the mitochondrial voltage dependent anion channel and promotes the release of cytochrome c.

Long Name

Bcl Associated X Protein

Entrez Gene IDs

581 (Human); 12028 (Mouse); 24887 (Rat)

Alternate Names

apoptosis regulator BAX; Bax; BCL2-associated X protein; Bcl2-L-4; BCL2L4bcl2-L-4; Bcl-2-like protein 4