Summary
Precision
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Rat IL-1 alpha/IL-1F1 Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL)
Sensitivity
4.12 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant rat IL-1 alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Rat IL-1 alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-1 alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat IL-1 alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant rat IL-1 alpha accurately. Results obtained using natural rat IL-1 alpha showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-1 alpha.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 54 | 134 | 417 | 54 | 130 | 449 |
| Standard Deviation | 3.6 | 6.3 | 16.5 | 5 | 9.5 | 29.7 |
| CV% | 6.7 | 4.7 | 4 | 9.3 | 7.3 | 6.6 |
Recovery
The recovery of rat IL-1 alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 104 | 100-111 |
| EDTA Plasma (n=5) | 98 | 89-112 |
| Serum (n=4) | 92 | 85-99 |
Linearity
To assess the linearity of the assay, samples spiked with rat IL-1 alpha in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-1 alpha/IL-1F1
IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signaling.
Long Name:
Interleukin 1 alpha
Entrez Gene IDs:
3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine); 101926546 (Cynomolgus Monkey)
Alternate Names:
BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Citations for Rat IL-1 alpha/IL-1F1 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Excessive Consumption Hibiscus sabdariffa L. Increases Inflammation and Blood Pressure in Male Wistar Rats via High Antioxidant Capacity: The Preliminary Findings
Authors: L Manzano-Pe, V Guarner-La, ME Soto, E Díaz-Díaz, S Caballero-, R Díaz-Torre, FL Rodríguez-, I Pérez-Torr
Cells, 2022-09-06;11(18):.
Species: Rat
Sample Types: Serum
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Nrf2 Suppression Delays Diabetic Wound Healing Through Sustained Oxidative Stress and Inflammation
Authors: M Li, H Yu, H Pan, X Zhou, Q Ruan, D Kong, Z Chu, H Li, J Huang, X Huang, A Chau, W Xie, Y Ding, P Yao
Front Pharmacol, 2019-09-20;10(0):1099.
Species: Rat
Sample Types: Serum
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Age-, Gender-, andin VivoDifferent Doses of Isoproterenol Modifyin VitroAortic Vasoreactivity and Circulating VCAM-1
Authors: B Nieto-Lima, A Cano-Martí, ME Rubio-Ruiz, I Pérez-Torr, V Guarner-La
Front Physiol, 2018-01-24;9(0):20.
Species: Mouse
Sample Types: Plasma
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Intraarterial route increases the risk of cerebral lesions after mesenchymal cell administration in animal model of ischemia
Authors: B Argibay, J Trekker, U Himmelreic, A Beiras, A Topete, P Taboada, M Pérez-Mato, A Vieites-Pr, R Iglesias-R, J Rivas, AM Planas, T Sobrino, J Castillo, F Campos
Sci Rep, 2017-01-16;7(0):40758.
Species: Rat
Sample Types: Serum
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Membrane-Permeable Calpain Inhibitors Promote Rat Oral Mucosal Epithelial Cell Proliferation by Inhibiting IL-1alpha Signaling.
Authors: Kondo M, Yamato M, Takagi R, Namiki H, Okano T
PLoS ONE, 2015-07-31;10(7):e0134240.
Species: Rat
Sample Types: Cell Culture Supernates
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Regulation of interleukin 1alpha, activin and inhibin by lipopolysaccharide in Sertoli cells from prepubertal rats.
Authors: Winnall WR, Okuma Y, Saito K
Mol. Cell. Endocrinol., 2009-02-28;307(1):169-75.
Species: Rat
Sample Types: Cell Culture Supernates
FAQs
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Rat IL-1 alpha/IL-1F1 Quantikine ELISA Kit
By Anonymous
on 05/30/2018
Sample Tested: Rat pancreatic islet lysate
