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Human Mer Antibody

Catalog #: MAB8912
25 Citations 2 Reviews Datasheet
Catalog # Availability Size / Price Qty
MAB8912-SP
MAB8912-100
MAB8912-500
Detection of Mer in HepG2 Human Cell Line by Flow Cytometry.
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Product Details
Citations (25)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Mer Antibody Summary

Species Reactivity
Human
Specificity
Detects human Mer in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Axl, rhDtk, or recombinant mouse Mer is observed.
Source
Monoclonal Mouse IgG2B Clone # 125518
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Mer
Met1-Ala499
Accession # AAB60430
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Knockout Validated
0.25 µg/106 cells
See below

Human Mer Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Mer Biotinylated Antibody (Catalog # BAF891)

Standard: Recombinant Human Mer Fc Chimera Protein, CF (Catalog # 891-MR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Mer antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Mer in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Mer Monoclonal Antibody (Catalog # MAB8912, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Flow Cytometry Detection of Mer antibody in U937 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Mer in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Mer Monoclonal Antibody (Catalog # MAB8912, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Knockout Validated Mer Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line. View Larger

Mer Specificity is Shown by Flow Cytometry in Knockout Cell Line. Mer knockout HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Mer Monoclonal Antibody (Catalog # MAB8912, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the Mer knockout HepG2 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Human Human Mer Antibody by Flow Cytometry View Larger

Detection of Human Human Mer Antibody by Flow Cytometry MR766 infection of unstimulated and decidualized T-HESC cell line.(a) T-HESC (left) and decidualized (d) T-HESC (right) were stained with ZIKV anti-dsRNA or E protein mAbs whereas the nuclei were stained with Hoechst. (b) Surface expression of AXL (red) and MER (blue) in T-HESC (left) and dT-HESC (right) was determined in uninfected cells by flow cytometry. The histograms of one experiment representative of 3 independently performed are shown. Double immunostaining for ZIKV E protein and calreticulin (c) or dsRNA and vimentin (d) in dT-HESC either uninfected or infected with MR766 at 72 h post-infection; Hoechst was used to stain nuclei. Arrows indicate localization of E protein in areas occupied by calreticulin. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28281680), licensed under a CC-BY license. Not internally tested by R&D Systems.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mer

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Recent studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions.

References
  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name
Receptor Tyrosine Protein Kinase Mer
Entrez Gene IDs
10461 (Human); 17289 (Mouse); 102143798 (Cynomolgus Monkey)
Alternate Names
c-Eyk; c-mer proto-oncogene tyrosine kinase; C-mer; EC 2.7.10; EC 2.7.10.1; MER receptor tyrosine kinase; Mer; MerTK; MGC133349; Receptor tyrosine kinase MerTK; RP38Proto-oncogene c-Mer; STK kinase; tyrosine-protein kinase Mer

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Citations for Human Mer Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. An adapted protocol to derive microglia from stem cells and its application in the study of CSF1R-related disorders
    Authors: Dorion, MF;Casas, D;Shlaifer, I;Yaqubi, M;Fleming, P;Karpilovsky, N;Chen, CX;Nicouleau, M;Piscopo, VEC;MacDougall, EJ;Alluli, A;Goldsmith, TM;Schneider, A;Dorion, S;Aprahamian, N;MacDonald, A;Thomas, RA;Dudley, RWR;Hall, JA;Fon, EA;Antel, JP;Stratton, JA;Durcan, TM;La Piana, R;Healy, LM;
    Molecular neurodegeneration
    Species: Human
    Sample Types: Whole Cells, Transfected Whole Cells
    Applications: Flow Cytometry
  2. Therapeutic Targeting of MERTK and BCL-2 in T-Cell and Early T-Precursor Acute Lymphoblastic Leukemia
    Authors: RJ Summers, J Jain, E Vasileiadi, B Smith, ML Chimenti, TY Yeung, J Kelvin, X Wang, SV Frye, HS Earp, JW Tyner, EC Dreaden, D DeRyckere, DK Graham
    Cancers, 2022-12-13;14(24):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Small Molecule Inhibitors of MERTK and FLT3 Induce Cell Cycle Arrest in Human CD8+ T Cells
    Authors: RM Powell, MJW Peeters, A Rahbech, P Aehnlich, T Seremet, P Thor Strat
    Vaccines, 2021-11-08;9(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. UNC5293, a potent, orally available and highly MERTK-selective inhibitor
    Authors: H Zheng, J Zhao, B Li, W Zhang, MA Stashko, KA Minson, MG Huey, Y Zhou, HS Earp, D Kireev, DK Graham, D DeRyckere, SV Frye, X Wang
    European Journal of Medicinal Chemistry, 2021-05-17;220(0):113534.
    Species: Human
    Sample Types: Cell Lysates
    Applications: IP
  5. A Potent and Selective Dual Inhibitor of AXL and MERTK Possesses Both Immunomodulatory and Tumor-Targeted Activity
    Authors: Jonathan Rios-Doria, Margaret Favata, Kerri Lasky, Patricia Feldman, Yvonne Lo, Gengjie Yang et al.
    Frontiers in Oncology
  6. MERTK Mediates Intrinsic and Adaptive Resistance to AXL-targeting Agents
    Authors: Nellie K. McDaniel, Christopher T. Cummings, Mari Iida, Justus Hülse, Hannah E. Pearson, Eleana Vasileiadi et al.
    Molecular Cancer Therapeutics
  7. The small‐molecule MERTK inhibitor UNC2025 decreases platelet activation and prevents thrombosis
    Authors: Brian R. Branchford, Timothy J. Stalker, Luke Law, Gilbert Acevedo, Susan Sather, Christine Brzezinski et al.
    Journal of Thrombosis and Haemostasis
  8. Cellular Differentiation of Human Monocytes Is Regulated by Time-Dependent Interleukin-4 Signaling and the Transcriptional Regulator NCOR2
    Authors: Jil Sander, Susanne V. Schmidt, Branko Cirovic, Naomi McGovern, Olympia Papantonopoulou, Anna-Lena Hardt et al.
    Immunity
  9. CD14(+)CD15(-)HLA-DR(-) myeloid-derived suppressor cells impair antimicrobial responses in patients with acute-on-chronic liver failure
    Authors: C Bernsmeier, E Triantafyl, R Brenig, FJ Lebosse, A Singanayag, VC Patel, OT Pop, W Khamri, R Nathwani, R Tidswell, CJ Weston, DH Adams, MR Thursz, JA Wendon, CG Antoniades
    Gut, 2017-06-07;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Human Endometrial Stromal Cells Are Highly Permissive To Productive Infection by Zika Virus
    Authors: Isabel Pagani, Silvia Ghezzi, Adele Ulisse, Alicia Rubio, Filippo Turrini, Elisabetta Garavaglia et al.
    Scientific Reports
  11. The MERTK/FLT3 inhibitor MRX-2843 overcomes resistance-conferring FLT3 mutations in acute myeloid leukemia
    Authors: Katherine A. Minson, Catherine C. Smith, Deborah DeRyckere, Clara Libbrecht, Alisa B. Lee-Sherick, Madeline G. Huey et al.
    JCI Insight
  12. The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles
    Authors: KE Happonen, S Tran, M Mörgelin, R Prince, S Calzavarin, A Angelillo-, B Dahlbäck
    J. Biol. Chem., 2016-03-22;291(20):10586-601.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Small Molecule Inhibition of MERTK Is Efficacious in Non–Small Cell Lung Cancer Models Independent of Driver Oncogene Status
    Authors: Christopher T. Cummings, Weihe Zhang, Kurtis D. Davies, Gregory D. Kirkpatrick, Dehui Zhang, Deborah DeRyckere et al.
    Molecular Cancer Therapeutics
  14. UNC2025, a Potent and Orally Bioavailable MER/FLT3 Dual Inhibitor
    Authors: Weihe Zhang, Deborah DeRyckere, Debra Hunter, Jing Liu, Michael A. Stashko, Katherine A. Minson et al.
    Journal of Medicinal Chemistry
  15. Mer590, a novel monoclonal antibody targeting MER receptor tyrosine kinase, decreases colony formation and increases chemosensitivity in non-small cell lung cancer
    Authors: Christopher T. Cummings, Rachel M.A. Linger, Rebecca A. Cohen, Susan Sather, Gregory D. Kirkpatrick, Kurtis D. Davies et al.
    Oncotarget
  16. MerTK inhibition is a novel therapeutic approach for glioblastoma multiforme
    Authors: Kristina H. Knubel, Ben M. Pernu, Alexandra Sufit, Sarah Nelson, Angela M. Pierce, Amy K. Keating
    Oncotarget
  17. Pseudo-Cyclization through Intramolecular Hydrogen Bond Enables Discovery of Pyridine Substituted Pyrimidines as New Mer Kinase Inhibitors
    Authors: Weihe Zhang, Dehui Zhang, Michael A. Stashko, Deborah DeRyckere, Debra Hunter, Dmitri Kireev et al.
    Journal of Medicinal Chemistry
  18. Discovery of Mer Specific Tyrosine Kinase Inhibitors for the Treatment and Prevention of Thrombosis
    Authors: Weihe Zhang, Andrew L. McIver, Michael A. Stashko, Deborah DeRyckere, Brian R. Branchford, Debra Hunter et al.
    Journal of Medicinal Chemistry
  19. UNC569, a Novel Small-Molecule Mer Inhibitor with Efficacy against Acute Lymphoblastic Leukemia In Vitro and In Vivo
    Authors: Sandra Christoph, Deborah DeRyckere, Jennifer Schlegel, J. Kimble Frazer, Lance A. Batchelor, Alesia Y. Trakhimets et al.
    Molecular Cancer Therapeutics
  20. Mer or Axl Receptor Tyrosine Kinase Inhibition Promotes Apoptosis, Blocks Growth, and Enhances Chemosensitivity of Human Non-Small Cell Lung Cancer
    Authors: Rachel M.A. Linger, Rebecca A. Cohen, Christopher T. Cummings, Susan Sather, Justine Migdall-Wilson, Deryck H.G. Middleton et al.
    Oncogene
  21. UNC1062, a new and potent Mer inhibitor
    Authors: Jing Liu, Weihe Zhang, Michael A. Stashko, Deborah DeRyckere, Christopher T. Cummings, Debra Hunter et al.
    European Journal of Medicinal Chemistry
  22. MERTK receptor tyrosine kinase is a therapeutic target in melanoma
    Authors: Jennifer Schlegel, Maria J. Sambade, Susan Sather, Stergios J. Moschos, Aik-Choon Tan, Amanda Winges et al.
    Journal of Clinical Investigation
  23. Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis.
    J. Exp. Med., 2012-10-15;209(11):2033-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Expression of the vitamin K-dependent proteins GAS6 and protein S and the TAM receptor tyrosine kinases in human atherosclerotic carotid plaques.
    Authors: Hurtado B, Munoz X, Recarte-Pelz P, Garcia N, Luque A, Krupinski J, Sala N, Garcia de Frutos P
    Thromb. Haemost., 2011-03-08;105(5):873-82.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  25. GAS6/Mer axis regulates the homing and survival of the E2A/PBX1-positive B-cell precursor acute lymphoblastic leukemia in the bone marrow niche.
    Authors: Shiozawa Y, Pedersen EA, Taichman RS
    Exp. Hematol., 2009-11-14;38(2):132-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human Mer Antibody

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Human Mer Antibody
By Sophie Lin on 04/26/2017
Human Mer Antibody
By sothy yi on 06/07/2016
Application: Func Sample Tested: NCI-H1299 Species: Human
Functional Assay Human Mer Antibody MAB8912