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Mouse IL-17/IL-17A Antibody

Catalog #: MAB721
25 Citations 2 Reviews Datasheet
Catalog # Availability Size / Price Qty
MAB721-SP
MAB721-100
MAB721-500
Detection of Recombinant Mouse and Rat IL‑17/IL‑17A by Western Blot.
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Citations (25)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-17/IL-17A Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-17 in ELISAs and Western blots. In direct ELISAs, 100% reactivity with recombinant mouse (rm) IL-17A/IL-17F heterodimer, 50% cross-reactivity with recombinant human IL-17, and 25% cross-reactivity with recombinant canine IL-17 is observed. No cross-reactivity with rmIL-17B, rmIL-17C, rmIL-17D, rmIL-17E, or rmIL-17F is observed. In Western blots, cross-reactivity with recombinant rat IL-17A is observed.
Source
Monoclonal Rat IgG2A Clone # 50101
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL‑17
Thr22-Ala158
Accession # Q62386
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Mouse IL-17 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse IL‑17 Biotinylated Antibody (Catalog # BAF421)

Standard: Recombinant Mouse IL-17A Protein (Catalog # 421-ML)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Recombinant Mouse and Rat IL-17/IL-17A antibody by Western Blot. View Larger

Detection of Recombinant Mouse and Rat IL‑17/IL‑17A by Western Blot. Western blot shows 25 ng of Recombinant Mouse IL-17/IL-17A (Catalog # 421-ML), Recombinant Human IL-17/IL-17A (Catalog # 317-ILB), Recombinant Rat IL-17/IL-17A (Catalog # 8410-IL), and Recombinant Mouse IL-17F (Catalog # 2057-IL). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-17/IL-17A Monoclonal Antibody (Catalog # MAB721) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-17/IL-17A at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17/IL-17A

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature  IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, mouse IL-17 shows 57% and 87% sequence identity with herpes virus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated alpha beta TCR+CD4-CD8- T cells, the expression of mouse IL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-kappa B and costimulation of T cell proliferation.

References
  1. Kennedy, J. et al. (1996) J. Interferon Cytokine Res. 16:611.
  2. Yao, Z. et al. (1995) J. Immunol. 155:5483.
  3. Yao, Z. et al. (1995) Immunity 3:811.
  4. Rouvier, E. et al. (1993) J. Immunol. 150:5445.
Long Name
Interleukin 17
Entrez Gene IDs
3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

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Citations for Mouse IL-17/IL-17A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. Kaempferol targeting on the fibroblast growth factor receptor 3-ribosomal S6 kinase 2 signaling axis prevents the development of rheumatoid arthritis
    Authors: CJ Lee, SJ Moon, JH Jeong, S Lee, MH Lee, SM Yoo, HS Lee, HC Kang, JY Lee, WS Lee, HJ Lee, EK Kim, JY Jhun, ML Cho, JK Min, YY Cho
    Cell Death Dis, 2018-03-14;9(3):401.
  2. Depletion of Foxp3+ regulatory T cells augments CD4+ T cell immune responses in atherosclerosis-prone hypercholesterolemic mice
    Authors: K Kasahara, N Sasaki, HZ Amin, T Tanaka, S Horibe, T Yamashita, KI Hirata, Y Rikitake
    Heliyon, 2022-07-19;8(7):e09981.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  3. Interleukin-17 affects synaptic plasticity and cognition in an experimental model of multiple sclerosis
    Authors: M Di Filippo, A Mancini, L Bellingacc, L Gaetani, P Mazzocchet, T Zelante, L La Barbera, A De Luca, M Tantucci, A Tozzi, V Durante, M Sciaccalug, A Megaro, D Chiasserin, N Salvadori, V Lisetti, E Portaccio, C Costa, P Sarchielli, MP Amato, L Parnetti, MT Viscomi, L Romani, P Calabresi
    Cell Reports, 2021-12-07;37(10):110094.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Electrophysiology
  4. Interferon-gamma regulates inflammatory cell death by targeting necroptosis in experimental autoimmune arthritis
    Authors: SH Lee, JY Kwon, SY Kim, K Jung, ML Cho
    Sci Rep, 2017-08-31;7(1):10133.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  5. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  6. The Major Histocompatibility Complex Class III Haplotype Ltab-Ncr3 Regulates Adjuvant-Induced but Not Antigen-Induced Autoimmunity
    Authors: AC Yau, J Tuncel, R Holmdahl
    Am. J. Pathol, 2017-03-18;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Capture)
  7. MHC class II alleles associated with Th1 rather than Th17 type immunity drive the onset of early arthritis in a rat model of rheumatoid arthritis
    Eur. J. Immunol, 2017-02-14;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  8. Targeting dendritic cells to accelerate T-cell activation overcomes a bottleneck in tuberculosis vaccine efficacy
    Nat Commun, 2016-12-22;7(0):13894.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISpot Development
  9. Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis
    Authors: Jung-Yeon Lim
    Sci Rep, 2016-06-01;6(0):26851.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  10. Positional identification of RT1-B (HLA-DQ) as susceptibility locus for autoimmune arthritis.
    Authors: Haag S, Tuncel J, Thordardottir S, Mason D, Yau A, Dobritzsch D, Backlund J, Peters E, Holmdahl R
    J Immunol, 2015-02-11;194(6):2539-50.
    Species: Rat
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  11. Immune requirements for protective Th17 recall responses to Mycobacterium tuberculosis challenge.
    Authors: Monin L, Griffiths K, Slight S, Lin Y, Rangel-Moreno J, Khader S
    Mucosal Immunol, 2015-01-28;8(5):1099-109.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISpot Development
  12. CD8(+) cells regulate the T helper-17 response in an experimental murine model of Sjogren syndrome.
    Authors: Zhang X, Schaumburg C, Coursey T, Siemasko K, Volpe E, Gandhi N, Li D, Niederkorn J, Stern M, Pflugfelder S, de Paiva C
    Mucosal Immunol, 2013-09-11;7(2):417-27.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISA Development (Capture)
  13. Heligmosomoides polygyrus bakeri Infection Activates Colonic Foxp3+ T Cells Enhancing Their Capacity To Prevent Colitis
    Authors: Long Hang, Arthur M. Blum, Tommy Setiawan, Joseph P. Urban, Korynn M. Stoyanoff, Joel V. Weinstock
    The Journal of Immunology
  14. Interferon gamma suppresses collagen-induced arthritis by regulation of Th17 through the induction of indoleamine-2,3-deoxygenase.
    Authors: Lee J, Lee J, Park M, Lim M, Park E, Kim E, Yang E, Lee S, Jhun J, Park S, Kim H, Cho M
    PLoS ONE, 2013-04-16;8(4):e60900.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  15. Heligmosomoides Polygyrus Abrogates Antigen-Specific Gut Injury in a Murine Model of Inflammatory Bowel Disease
    Authors: John Leung, Long Hang, Arthur Blum, Tommy Setiawan, Korynn Stoyanoff, Joel Weinstock
    Inflammatory Bowel Diseases
  16. CCL25 induces alpha 4 beta 7 integrin-dependent migration of IL-17+ gamma/delta T lymphocytes during an allergic reaction.
    Authors: Costa MF, Bornstein VU, Candea AL, Henriques-Pons A, Henriques MG, Penido C
    Eur. J. Immunol., 2012-05-01;42(5):1250-60.
    Species: Mouse
    Sample Types: Pleural Lavage Fluid
    Applications: ELISA Development
  17. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012-01-25;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  18. TGF-beta regulates T-cell neurokinin-1 receptor internalization and function
    Authors: Martin Beinborn, Arthur Blum, Long Hang, Tommy Setiawan, Jonathan C. Schroeder, Korynn Stoyanoff et al.
    Proceedings of the National Academy of Sciences
  19. Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
    Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
    Proc. Natl. Acad. Sci. U.S.A., 2009-03-16;106(14):5954-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  20. Tumor-specific Th17-polarized cells eradicate large established melanoma.
    Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
    Blood, 2008-03-19;112(2):362-73.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  21. The proinflammatory effect of prostaglandin E2 in experimental inflammatory bowel disease is mediated through the IL-23-->IL-17 axis.
    Authors: Sheibanie AF, Yen JH, Khayrullina T, Emig F, Zhang M, Tuma R, Ganea D
    J. Immunol., 2007-06-15;178(12):8138-47.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  22. Interleukin (IL)-22 and IL-17 are coexpressed by Th17 cells and cooperatively enhance expression of antimicrobial peptides
    Authors: Spencer C. Liang, Xiang-Yang Tan, Deborah P. Luxenberg, Riyez Karim, Kyriaki Dunussi-Joannopoulos, Mary Collins et al.
    The Journal of Experimental Medicine
  23. IL-17 production is dominated by gammadelta T cells rather than CD4 T cells during Mycobacterium tuberculosis infection.
    Authors: Lockhart E, Green AM, Flynn JL
    J. Immunol., 2006-10-01;177(7):4662-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISpot Development
  24. IL-23 compensates for the absence of IL-12p70 and is essential for the IL-17 response during tuberculosis but is dispensable for protection and antigen-specific IFN-gamma responses if IL-12p70 is available.
    Authors: Khader SA, Pearl JE, Sakamoto K, Gilmartin L, Bell GK, Jelley-Gibbs DM, Ghilardi N, deSauvage F, Cooper AM
    J. Immunol., 2005-07-15;175(2):788-95.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISpot Development
  25. IL-1-independent role of IL-17 in synovial inflammation and joint destruction during collagen-induced arthritis.
    Authors: Lubberts E, Joosten LA, Oppers B, van den Bersselaar L, Coenen-de Roo CJ, Kolls JK, Schwarzenberger P, van de Loo FA, van den Berg WB
    J. Immunol., 2001-07-15;167(2):1004-13.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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Mouse IL-17/IL-17A Antibody
By Anonymous on 11/27/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Mouse
ELISA Mouse IL-17/IL-17A Antibody MAB721
Mouse IL‑17/IL‑17A Antibody
By Boubacar Barry on 08/11/2017
Application: Affinity Purification Sample Tested: Blood erythrocytes Species: Drosophila