Human ICAM-1/CD54 Antibody Summary
Extracellular domain
Applications
under non-reducing conditions only
Human ICAM-1/CD54 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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ICAM‑1/CD54 in A431 Human Cell Line. ICAM-1/CD54 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human ICAM-1/CD54 Monoclonal Antibody (Catalog # MAB720) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-1/CD54
Intercellular Adhesion Molecule-1 (ICAM-1) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be upregulated upon cell activation.
Product Datasheets
Citations for Human ICAM-1/CD54 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
5
Citations: Showing 1 - 5
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An Anti-Human ICAM-1 Antibody Inhibits Rhinovirus-Induced Exacerbations of Lung Inflammation
Authors: Stephanie Traub, Alexandra Nikonova, Alan Carruthers, Rebecca Dunmore, Katherine A. Vousden, Leila Gogsadze et al.
PLoS Pathogens
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High plasma levels of soluble intercellular adhesion molecule (ICAM)-1 are associated with cerebral malaria.
Authors: Adukpo, Selorme, Kusi, Kwadwo A, Ofori, Michael, Tetteh, John K A, Amoako-Sakyi, Daniel, Goka, Bamenla, Adjei, George O, Edoh, Dominic, Akanmori, Bartholo, Gyan, Ben A, Dodoo, Daniel
PLoS ONE, 2013-12-27;8(12):e84181.
Species: Human
Sample Types: Plasma
Applications: ELISA Development -
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008-04-19;7(6):2406-14.
Species: Human
Sample Types: Serum
Applications: ELISA Microarray Development -
Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Authors: Pfleger C, Schloot N, ter Veld F
J. Immunol. Methods, 2007-10-22;329(1):214-8.
Species: Human
Sample Types: Serum
Applications: Luminex Development -
Neutrophil microvesicles drive atherosclerosis by delivering miR-155 to atheroprone endothelium
Authors: I Gomez, B Ward, C Souilhol, C Recarti, M Ariaans, J Johnston, A Burnett, M Mahmoud, LA Luong, L West, M Long, S Parry, R Woods, C Hulston, B Benedikter, C Niespolo, R Bazaz, S Francis, E Kiss-Toth, M van Zandvo, A Schober, P Hellewell, PC Evans, V Ridger
Nat Commun, 2020-01-10;11(1):214.
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