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Human CXCL11/I-TAC Antibody

Catalog #: MAB672
13 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB672-SP
MAB672-100
MAB672-500
Chemotaxis Induced by CXCL11/I‑TAC and Neutralization by Human CXCL11/I‑TAC Antibody.
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Citations (13)
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Human CXCL11/I-TAC Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCL11/I-TAC in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) CXCL5, 7, 8, 9, or 10. In Western blots, this antibody shows less than 1% cross-reactivity with rhCXCL1, 2, 3, 5, 6, 7, IL-8/CXCL8, 9, 10, 13, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, rmCXCL1, 2, 6, 9, 10, CXCL12/SDF-1 alpha, rmCXCL13, rrCXCL1, CXCL3/CINC-2 alpha, CXCL3/CINC-2 beta, or rpIL-8/CXCL8.
Source
Monoclonal Mouse IgG2A Clone # 87328
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human CXCL11/I-TAC
Phe22-Phe94
Accession # O14625
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CXCL11/I-TAC (Catalog # 672-IT)

Human CXCL11/I-TAC Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human CXCL11/I-TAC Biotinylated Antibody (Catalog # BAF672)

Standard: Recombinant Human CXCL11/I-TAC Protein (Catalog # 672-IT)

Neutralization
Measured by its ability to neutralize CXCL11/I-TAC-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR3. The Neutralization Dose (ND50) is typically 0.0400-0.480 μg/mL in the presence of 15 ng/mL Recombinant Human CXCL11/I-TAC.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CXCL11/I‑TAC and Neutralization by Human CXCL11/I‑TAC Antibody. View Larger

Chemotaxis Induced by CXCL11/I‑TAC and Neutralization by Human CXCL11/I‑TAC Antibody. Recombinant Human CXCL11/I‑TAC (672-IT) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CXCL11/I‑TAC (15 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL11/I-TAC Monoclonal Antibody (Catalog # MAB672). The ND50 is typically 0.0400‑0.480 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL11/I-TAC

CXCL11, also known as I-TAC, SCYB9B, H174 and beta -R1, is a non-ELR CXC chemokine. CXCL11 cDNA encodes a 94 amino acid (aa) residue precursor protein with a 21 aa residue putative signal sequence, which is cleaved to form the mature 73 aa residue protein. CXCL11 shares 36% and 37% amino acid sequence homology with IP-10 and MIG (two other known human non-ELR CXC chemokines), respectively. CXCL11 is expressed at low levels in normal tissues including thymus, spleen, and pancreas. The expression of CXCL11 mRNA is radically up regulated in IFN-gamma and IL-1 stimulated astrocytes. Moderate increase in expression is also observed in stimulated monocytes. CXCL11 has potent chemoattractant activity for IL-2 activated T cells and transfected cell lines expressing CXCR3 but not freshly isolated T cells, neutrophils, or monocytes. The gene encoding CXCL11 has been mapped to chromosome 4.

References
  1. Cole, K. et al. (1998) J. Exp. Med. 187:2009.
  2. Sandhya Rani, M. et al. (1996) J. Biol. Chem. 271:22878.
  3. Lou, Y. et al. (1998) J. Neurovirol. 4:575.
Entrez Gene IDs
6373 (Human); 56066 (Mouse)
Alternate Names
beta-R1; b-R1; chemokine (C-X-C motif) ligand 11; CXCL11; H174; H174IP9; Interferon gamma-inducible protein 9; Interferon-inducible T-cell alpha chemoattractant; IP-9member 11; ITAC; I-TAC; I-TACMGC102770; SCYB9B; small inducible cytokine subfamily B (Cys-X-Cys), member 9B; Small-inducible cytokine B11

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Citations for Human CXCL11/I-TAC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Pathogen Evasion of Chemokine Response Through Suppression of CXCL10
    Authors: Alejandro L. Antonia, Kyle D. Gibbs, Esme D. Trahair, Kelly J. Pittman, Amelia T. Martin, Benjamin H. Schott et al.
    Frontiers in Cellular and Infection Microbiology
  2. Epigenetic Repression of STING by MYC Promotes Immune Evasion and Resistance to Immune Checkpoint Inhibitors in Triple-Negative Breast Cancer
    Authors: Kyung-min Lee, Chang-Ching Lin, Alberto Servetto, Joonbeom Bae, Vishal Kandagatla, Dan Ye et al.
    Cancer Immunology Research
  3. Blockade of Immune Checkpoint B7-H4 and Lysine Demethylase 5B in Esophageal Squamous Cell Carcinoma Confers Protective Immunity against P. gingivalis Infection
    Authors: X Yuan, Y Liu, G Lee, Z Lan, M Ma, H Lee, J Kong, J Sun, G Hou, X Hou, Y Ma, F Ren, F Zhou, S Gao
    Cancer Immunol Res, 2019-07-26;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  4. Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation.
    Authors: Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R
    BMC Cancer, 2014-02-22;14(0):118.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010-02-17;184(6):2886-98.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome.
    Authors: Yoon KC, Park CS, You IC, Choi HJ, Lee KH, Im SK, Park HY, Pflugfelder SC
    Invest. Ophthalmol. Vis. Sci., 2009-10-22;51(2):643-50.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients.
    Authors: Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D
    J. Immunol., 2007-09-15;179(6):4272-82.
    Species: Human
    Sample Types: Serum, Whole Cells
    Applications: ICC, Immunodepletion
  8. CXCR 3 activation promotes lymphocyte transendothelial migration across human hepatic endothelium under fluid flow.
    Authors: Curbishley SM, Eksteen B, Gladue RP, Lalor P, Adams DH
    Am. J. Pathol., 2005-09-01;167(3):887-99.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  9. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
    Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
    J. Immunol., 2004-12-01;173(11):6592-602.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  10. Enhanced generation of helper T type 1 and 2 chemokines in allergen-induced asthma.
    Authors: Liu L, Jarjour NN, Busse WW, Kelly EA
    Am. J. Respir. Crit. Care Med., 2004-03-04;169(10):1118-24.
    Species: Human
    Sample Types: BALF
    Applications: ELISA Development
  11. PRMT5-Mediated Methylation of NF-kappa B p65 at Arg174 Is Required for Endothelial CXCL11 Gene Induction in Response to TNF-alpha and IFN-gamma Costimulation
    Authors: Daniel P. Harris, Unnikrishnan M. Chandrasekharan, Smarajit Bandyopadhyay, Belinda Willard, Paul E. DiCorleto
    PLOS ONE
  12. Herpes Simplex Virus Type 2 Infection-Induced Expression of CXCR3 Ligands Promotes CD4+ T Cell Migration and Is Regulated by the Viral Immediate-Early Protein ICP4
    Authors: Mudan Zhang, Xu Deng, Xinmeng Guan, Lanlan Geng, Ming Fu, Binman Zhang et al.
    Frontiers in Immunology
  13. Hypoxia-immune-related microenvironment prognostic signature for osteosarcoma
    Authors: Wenshuo Zhang, Pang Lyu, Darja Andreev, Yewei Jia, Fulin Zhang, Aline Bozec
    Frontiers in Cell and Developmental Biology

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