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Rat VEGF164 Antibody

Catalog #: MAB564
4 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB564-SP
MAB564-500
MAB564-100
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat VEGF164 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat VEGF164 in direct ELISAs and Western blots. Shows 50 - 100% cross-reactivity with recombinant mouse (rm) VEGF120, rmVEGF164, recombinant human (rh) VEGF121, rhVEGF165, and rhVEGF189 and no cross-reactivity with rmVEGF115. Recognizes an epitope found between amino acids 78-120 of rmVEGF120. This segment is present in all VEGF isoforms except rmVEGF115.
Source
Monoclonal Mouse IgG2B Clone # 123704
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat VEGF164
Ala27-Arg190
Accession # AAA41211.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Rat VEGF164 (Catalog # 564-RV)
under non-reducing conditions only

Rat VEGF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Rat VEGF Biotinylated Antibody (Catalog # BAF564)

Standard: Recombinant Rat VEGF 164 Protein (Catalog # 564-RV)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF

Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF164 appears to be the most abundant and potent isoform, followed by VEGF120 and VEGF188. Rat VEGF164 is an approximately 50 kDa molecular weight homodimer sharing 97% aa sequence identity with corresponding regions of mouse, 88% with human and bovine, 90% with feline and equine, and 89% with canine and porcine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. Human VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.

Long Name
Vascular Endothelial Growth Factor
Entrez Gene IDs
7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

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Citations for Rat VEGF164 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Protective Effects of Mesenchymal Stem Cells Overexpressing Extracellular Regulating Kinase 1/2 Against Stroke in Rats
    Authors: X Gao, D Wu, L Dou, H Zhang, L Huang, J Zeng, Y Zhang, C Yang, H Li, L Liu, B Ma, Q Yuan
    Brain Res. Bull., 2019-04-16;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Platelet rich plasma clot releasate preconditioning induced PI3K/AKT/NFkappaB signaling enhances survival and regenerative function of rat bone marrow mesenchymal stem cells in hostile microenvironments.
    Authors: Peng Y, Huang S, Wu Y, Cheng B, Nie X, Liu H, Ma K, Zhou J, Gao D, Feng C, Yang S, Fu X
    Stem Cells Dev, 2013-08-30;22(24):3236-51.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  3. Topical administration of nepafenac inhibits diabetes-induced retinal microvascular disease and underlying abnormalities of retinal metabolism and physiology.
    Authors: Kern TS, Miller CM, Du Y, Zheng L, Mohr S, Ball SL, Kim M, Jamison JA, Bingaman DP
    Diabetes, 2007-02-01;56(2):373-9.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Aspirin inhibits endothelial nitric oxide synthase (eNOS) and Flk-1 (vascular endothelial growth factor receptor-2) prior to rat colon tumour development.
    Authors: Escribano M, Molero L, Lopez-Farre A, Abarrategui C, Carrasco C, Garcia-Mendez A, Manzarbeitia F, Martin MJ, Vazquez M, Sanchez-Fayos P, Rico L, Porres Cubero JC
    Clin. Sci., 2004-01-01;106(1):83-91.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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