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Rat CNTF Antibody

Catalog #: MAB557
2 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB557
MAB557-SP
Cell Proliferation Induced by CNTF and Neutralization by Rat CNTF Antibody.
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Citations (2)
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Rat CNTF Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat CNTF in ELISAs and Western blots. In sandwich immunoassays, less than 3% cross-reactivity with recombinant human CNTF is observed.
Source
Monoclonal Mouse IgG2B Clone # 34738
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant rat CNTF
Ala2-Met200
Accession # P20294.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<1.0 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample

Rat CNTF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Rat CNTF Biotinylated Antibody (Catalog # BAF557)

Standard: Recombinant Rat CNTF Protein (Catalog # 557-NT)

Neutralization
Measured by its ability to neutralize CNTF-induced proliferation in the TF‑1 human erythroleukemic cell line. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 200 ng/mL Recombinant Rat CNTF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by CNTF and Neutralization by Rat CNTF Antibody. View Larger

Cell Proliferation Induced by CNTF and Neutralization by Rat CNTF Antibody. Recombinant Rat CNTF (Catalog # 557-NT) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat CNTF (200 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CNTF Monoclonal Antibody (Catalog # MAB557). The ND50 is typically 1-3 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CNTF

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons, and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. The cDNA for CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.

Long Name
Ciliary Neurotrophic Factor
Entrez Gene IDs
1270 (Human); 12803 (Mouse); 25707 (Rat)
Alternate Names
ciliary neurotrophic factor; CNTF; HCNTF

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Citations for Rat CNTF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Ciliary neurotrophic factor signaling in the rat orbitofrontal cortex ameliorates stress-induced deficits in reversal learning
    Authors: Girotti M, Silva JD, George CM, Morilak DA
    Neuropharmacology
  2. Microglia-derived interleukin-6 and leukaemia inhibitory factor promote astrocytic differentiation of neural stem/progenitor cells.
    Authors: Nakanishi M, Niidome T, Matsuda S, Akaike A, Kihara T, Sugimoto H
    Eur. J. Neurosci., 2007-02-01;25(3):649-58.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Immunodepletion

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