Rat CCL20/MIP-3 alpha Antibody

Catalog #: MAB540
2 Citations 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
MAB540
MAB540-SP

Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Rat CCL20/MIP‑3 alpha Antibody.

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All CCL20/MIP-3 alpha Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat CCL20/MIP-3 alpha Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CCL20/MIP-3 alpha in ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 83225

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant rat CCL20/MIP-3 alpha
Ala26-Met96
Accession # P97884.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.20 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Rat CCL20/MIP‑3 alpha (Catalog # 540-RM)
under non-reducing conditions only

Rat CCL20/MIP-3 alpha Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Rat CCL20/MIP‑3 alpha Biotinylated Antibody (Catalog # BAF540)

Standard: Recombinant Rat CCL20/MIP-3 alpha Protein (Catalog # 540-RM)

Neutralization

Measured by its ability to neutralize CCL20/MIP‑3 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR6. The Neutralization Dose (ND₅₀) is typically 20-40 µg/mL in the presence of 5 µg/mL Recombinant Rat CCL20/MIP‑3 alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Rat CCL20/MIP‑3 alpha Antibody. Recombinant Rat CCL20/MIP-3a (Catalog # 540-RM) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CCL20/MIP-3a (5 µg/mL) is neutralized (green line) by increasing concentrations of Rat CCL20/MIP-3a Monoclonal Antibody (Catalog # MAB540). The ND₅₀ is typically 20-40 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL20/MIP-3 alpha

CCL20, also known as MIP-3 alpha, LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. Rat CCL20 cDNA encodes a 96 amino acid (aa) precursor protein with a 25 aa putative signal peptide that is predicted to be cleaved to form the 71 aa mature secreted protein. CCL20 is distantly related to other beta chemokines (20-28% aa sequence identity). Rat MIP-3 alpha shares approximately 70% and 61% aa sequence homology with mouse and human CCL20, respectively.

CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34⁺ cord blood precursors.

References

Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
Hromas, R. et al. (1997) Blood 89:3315.
Greaves, D.R. et al. (1997) J. Exp. Med. 186: 857.
Tanaka, Y. et al. (1999) Eur. J. Immunol. 29:633.

Entrez Gene IDs

6364 (Human); 20297 (Mouse); 29538 (Rat)

Alternate Names

beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38

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Related Research Areas

Citations for Rat CCL20/MIP-3 alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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