Rat IL-4 Antibody

Catalog #: MAB504
3 Citations 1 Review Datasheet

Catalog #	Availability	Size / Price	Qty
MAB504-SP
MAB504-100
MAB504-500

All IL-4 Antibodies

Product Details

Citations (3)

FAQs

Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat IL-4 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat IL-4 in ELISAs and Western blots. Does not cross-react with recombinant human IL‑4 or recombinant mouse IL‑4.

Source

Monoclonal Mouse IgG_2B Clone # 56567

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant rat IL‑4
Cys25-Ser147
Accession # P20096.2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Rat IL‑4 (Catalog # 504-RL)

Rat IL-4 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Rat IL‑4 Biotinylated Antibody (Catalog # BAF504)

Standard: Recombinant Rat IL-4 Protein (Catalog # 504-RL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa - 18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1-3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Rat IL-4 is synthesized with a 24 aa signal sequence. Mature rat IL-4 shares 41%, 43%, and 59% aa sequence identity with bovine, human, and mouse IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5-7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4⁺ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in rodent B cells, acquisition of the Th2 phenotype by naïve CD4⁺ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10-13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

References

Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
McKnight, A.J. et al. (1991) Eur. J. Immunol. 21:1187.
Redfield, C. et al. (1991) Biochemistry 30:11029.
Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
Corthay, A. (2006) Scand. J. Immunol. 64:93.
Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

Long Name

Interleukin 4

Entrez Gene IDs

3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)

Alternate Names

B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Rat IL-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Adenine Nucleotide Translocase 1 Expression Modulates the Immune Response in Ischemic Hearts
Authors: F Yergöz, J Friebel, N Kränkel, U Rauch-Kroe, HP Schultheis, U Landmesser, A Dörner
Cells, 2021-08-19;10(8):.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot
The role of cyclosporine and mycophenolate in an orthotopic porcine-to-rat corneal xenotransplantation.
Authors: Lee HI, Kim MK, Oh JY, Ko JH, Lee HJ, Wee WR, Lee JH
J. Korean Med. Sci., 2008-06-01;23(3):492-501.
Species: Rat
Sample Types: Tissue Homogenates
Applications: ELISA Development
Overexpression of interleukin-13 induces minimal-change-like nephropathy in rats.
Authors: Lai KW, Wei CL, Tan LK, Tan PH, Chiang GS, Lee CG, Jordan SC, Yap HK
J. Am. Soc. Nephrol., 2007-04-11;18(5):1476-85.
Species: Rat
Sample Types: Serum
Applications: ELISA Development