Mouse IL-12/IL-23 p40 Antibody

Catalog #: MAB4991
9 Citations 2 Reviews Datasheet

Catalog #	Availability	Size / Price	Qty
MAB4991-SP
MAB4991-100
MAB4991-500

Cell Proliferation Induced by IL‑12 and Neutralization by Mouse IL‑12/IL‑23 p40 Antibody.

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All IL-12/IL-23 p40 Antibodies

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Citations (9)

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Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-12/IL-23 p40 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects the mouse IL-12/IL-23 p40 subunit. Polymorphisms exist in the mouse p40 sequences [Ymer, S. et al. (2002) Genes and Immunity 3:151.].

Source

Monoclonal Rat IgG_2A Clone # C17.8

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Recombinant mouse IL-12 p70 heterodimer

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse IL-12/IL-23 p40 Homodimer (Catalog # 499-ML)
under non-reducing conditions only

Mouse IL-12/IL-23 p40 Nonallele-specific Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse IL‑12/IL‑23 p40 Biotinylated Antibody (Catalog # BAF499)

Standard: Recombinant Mouse IL-12/IL-23 p40 Homodimer Protein (Catalog # 499-ML)

Neutralization

Measured by its ability to neutralize IL‑12-induced proliferation in PHA-activated mouse splenocytes. The Neutralization Dose (ND₅₀) is typically 0.04-0.3 µg/mL in the presence of 1 ng/mL Recombinant Mouse IL‑12.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

View Larger

Cell Proliferation Induced by IL‑12 and Neutralization by Mouse IL‑12/IL‑23 p40 Antibody. Recombinant Mouse IL-12 (Catalog # 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-12/IL-23 p40 Monoclonal Antibody (Catalog # MAB4991). The ND₅₀ is typically 0.04-0.3 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12/IL-23 p40

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 has multiple effects on T cells and NK cells and is a key mediator in the development of Th1 cells.

IL-12 is a heterodimeric cytokine containing two disulfide-linked subunits, p35 and p40. Human and mouse IL-12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. Although mouse IL-12 is active on human or mouse IL-12 responsive cells, human IL-12 is not active on mouse cells.

The disulfide-linked mouse p40 homodimer can bind to IL-12 receptors and is an antagonist of IL-12 activities in vitro. The mouse p40 monomer is at least ten times less active than the homodimer as an IL-12 antagonist. At the present time, the existence and the physiological role of mouse p40 homodimer in vivo remains to be determined.

Long Name

Interleukin 12/Interleukin 23 p40

Entrez Gene IDs

3593 (Human); 16160 (Mouse); 64546 (Rat); 397076 (Porcine); 403976 (Canine); 102124858 (Cynomolgus Monkey); 493741 (Feline); 694747 (Rhesus Macaque)

Alternate Names

CLMF p40; CLMF; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL12 p40; IL-12 p40; IL-12 subunit p40; IL12B; IL-12B; IL-12BNK cell stimulatory factor chain 2; interleukin 12, p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40); interleukin-12 beta chain; interleukin-12 subunit beta; natural killer cell stimulatory factor, 40 kD subunit; NKSF; NKSF2; NKSF2IL12, subunit p40

Product Datasheets

Product Datasheet

Related Research Areas

Citations for Mouse IL-12/IL-23 p40 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Transgenic viral expression of PH-20, IL-12, and sPD1-Fc enhances immune cell infiltration and anti-tumor efficacy of an oncolytic virus
Authors: Soon-Oh Hong, Joonsung Kim, Sungmin Lee, Jaeil Shin, Hwanjun Choi, Eunjin Lee et al.
Mol Ther Oncolytics
IFN-?-Expressing Th17 Cells Are Required for Development of Severe Ocular Surface Autoimmunity
Authors: Y Chen, SK Chauhan, C Shao, M Omoto, T Inomata, R Dana
J. Immunol., 2017-06-21;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections.
Authors: Ohama H, Asai A, Ito I, Suzuki S, Kobayashi M, Higuchi K, Suzuki F
Am J Pathol, 2014-12-06;185(2):420-31.
Species: Mouse
Sample Types: Whole Cells
Applications: Functional Assay
Co-adjuvant effects of retinoic acid and IL-15 induce inflammatory immunity to dietary antigens.
Authors: DePaolo RW, Abadie V, Tang F
Nature, 2011-02-09;471(7337):220-4.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Neutralization
The pro-Th2 cytokine IL-33 directly interacts with invariant NKT and NK cells to induce IFN-gamma production.
Authors: Bourgeois E, Van LP, Samson M, Diem S, Barra A, Roga S, Gombert JM, Schneider E, Dy M, Gourdy P, Girard JP, Herbelin A
Eur. J. Immunol., 2009-04-01;39(4):1046-55.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
TSU68 prevents liver metastasis of colon cancer xenografts by modulating the premetastatic niche.
Authors: Yamamoto M, Kikuchi H, Ohta M, Kawabata T, Hiramatsu Y, Kondo K, Baba M, Kamiya K, Tanaka T, Kitagawa M, Konno H
Cancer Res., 2008-12-01;68(23):9754-62.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages.
Authors: An HJ, Jeong HJ, Um JY, Kim HM, Hong SH
J Ethnopharmacol, 2006-03-10;106(3):418-24.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development
17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
Blood, 2004-05-13;104(5):1404-10.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12.
Authors: Oppmann, B, Lesley, R, Blom, B, Timans, J C, Xu, Y, Hunte, B, Vega, F, Yu, N, Wang, J, Singh, K, Zonin, F, Vaisberg, E, Churakova, T, Liu, M, Gorman, D, Wagner, J, Zurawski, S, Liu, Y, Abrams, J S, Moore, K W, Rennick, D, de Waal-Malefyt, R, Hannum, C, Bazan, J F, Kastelein, R A
Immunity, 2000-11-01;13(5):715-25.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry