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Mouse LIX Antibody

Catalog #: MAB433
14 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB433-SP
MAB433-100
MAB433-500
Chemotaxis Induced by LIX and Neutralization by Mouse LIX Antibody.
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Citations (14)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse LIX Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse LIX in Western blots. In Western blots, no cross-reactivity with recombinant human CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12/SDF-1 alpha, 12/SDF-1 beta, 13, recombinant mouse CXCL1, 2, 6, 9, 10, 12/SDF-1 alpha, 13, recombinant rat CXCL1, 3/CINC-2 alpha, 3/CINC-2 beta, or recombinant porcine CXCL8 is observed.
Source
Monoclonal Rat IgG2B Clone # 61905
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse LIX
Val45-Ala118
Accession # P50228
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse LIX (Catalog # 433-MC)
under non-reducing conditions only

Mouse LIX Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse LIX Biotinylated Antibody (Catalog # BAF433)

Standard: Recombinant Mouse LIX Protein (Catalog # 433-MC)

Neutralization
Measured by its ability to neutralize LIX-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 0.2 µg/mL Recombinant Mouse LIX.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by LIX and Neutralization by Mouse LIX Antibody. View Larger

Chemotaxis Induced by LIX and Neutralization by Mouse LIX Antibody. Recombinant Mouse LIX (Catalog # 433-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse LIX (0.2 µg/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse LIX Monoclonal Antibody (Catalog # MAB433). The ND50 is typically 0.5-2.5 µg/mL.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIX

LIX (Liposaccharide-Induced CXC chemokine; also GARG-8) is a secreted 8-9 kDa member of the Intercrine alpha (or CxC) family of chemokines.  It is widely expressed, being produced by diverse cell types such as fibroblasts, thymic epithelium, platelets, vascular endothelium, hepatocytes, lung type II alveolar cells and ileal columnar epithelium.  As a chemokine, LIX demonstrates chemokinetic properties.  It induces the chemotaxis of neutrophils and endothelial cells, and also promotes TNF-alpha secretion from mast cells and macrophages.  Notably, circulating LIX is not derived from fibroblasts, but platelets.  This suggests that neutrophil homeostasis/chemotaxis is a function of local resident cell activation and LIX secretion, not generally circulating LIX.  Mouse LIX is synthesized as a 132 amino acid (aa) precursor that contains a 40 aa signal sequence, a 78 aa mature region (aa 41-118), and a cleavable 14 aa C-terminus.  The mature region possesses an ELR/GluLeuArg motif between aa 50-52, and an alpha -family characteristic CxC motif between aa 53-55.  Although there are no known splice variants of mouse LIX, considerable proteolytic processing occurs at both the N- and C-termini over aa 41-132.  This may reduce the MW in SDS-PAGE by as much as 3 kDa.  The majority of LIX appears to start between aa 47-50, and this is positively correlated with bioactivity.  Over aa 41-118, mouse LIX shares 73% aa sequence identity with rat LIX.  Although not a strict ortholog, mouse LIX shares 63% aa sequence identity with human GCP-2.

Entrez Gene IDs
20311 (Mouse); 60665 (Rat)
Alternate Names
LIX

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Citations for Mouse LIX Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. CXCR1 drives the pathogenesis of EAE and ARDS via boosting dendritic cells-dependent inflammation
    Authors: Zhuang, W;Zhou, J;Zhong, L;Lv, J;Zhong, X;Liu, G;Xie, L;Wang, C;Saimaier, K;Han, S;Shi, C;Hua, Q;Zhang, R;Xie, X;Du, C;
    Cell death & disease
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo assay
  2. CXCL5 suppression recovers neovascularization and accelerates wound healing in diabetes mellitus
    Authors: Chen, C;Lin, LY;Chen, JW;Chang, TT;
    Cardiovascular diabetology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  3. IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury
    Authors: G Xiao, R Kumar, Y Komuro, J Burguet, V Kakarla, I Azizkhania, SA Sheth, CK Williams, XR Zhang, M Macknicki, A Brumm, R Kawaguchi, P Mai, N Kaneko, HV Vinters, ST Carmichael, LA Havton, C DeCarli, JD Hinman
    Cell Reports, 2022-12-20;41(12):111848.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Early growth response 1 deficiency protects host against Pseudomonas aeruginosa lung infection
    Authors: Z Pang, R Raudonis, C McCormick, Z Cheng
    Infect. Immun., 2019-12-17;0(0):.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Capture
  5. The CXCL5/CXCR2 axis is sufficient to promote breast cancer colonization during bone metastasis
    Authors: R Romero-Mor, KJ Curtis, TR Coughlin, M Cristina M, S Dutta, A Natarajan, BA Facchine, KM Jackson, L Nystrom, J Li, W Kaliney, GL Niebur, LE Littlepage
    Nat Commun, 2019-09-27;10(1):4404.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Pulmonary Epithelial TLR4 Activation Leads to Lung Injury in Neonatal Necrotizing Enterocolitis
    Authors: Hongpeng Jia
    J Immunol, 2016-06-15;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  7. Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
    Authors: Junkins R, MacNeil A, Wu Z, McCormick C, Lin T
    J Immunol, 2013-04-15;190(10):5178-86.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development (Capture)
  8. In vivo processing of CXCL5 (LIX) by matrix metalloproteinase (MMP)-2 and MMP-9 promotes early neutrophil recruitment in IL-1beta-induced peritonitis.
    Authors: Song J, Wu C, Zhang X, Sorokin L
    J Immunol, 2012-12-07;190(1):401-10.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  9. Castration-induced changes in mouse epididymal white adipose tissue.
    Authors: Floryk D, Kurosaka S, Tanimoto R, Yang G, Goltsov A, Park S, Thompson TC
    Mol. Cell. Endocrinol., 2011-07-12;345(1):58-67.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Matrix metalloproteinase-8 deficiency promotes granulocytic allergen-induced airway inflammation.
    Authors: Gueders MM, Balbin M, Rocks N, Foidart JM, Gosset P, Louis R, Shapiro S, Lopez-Otin C, Noel A, Cataldo DD
    J. Immunol., 2005-08-15;175(4):2589-97.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. Transcriptional profiling of lipopolysaccharide-induced acute lung injury.
    Authors: Jeyaseelan S, Chu HW, Young SK, Worthen GS
    Infect. Immun., 2004-12-01;72(12):7247-56.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Cells
    Applications: ELISA Development, Neutralization
  12. Circulating CXC-chemokine concentrations in a murine intestinal ischemia-reperfusion model.
    Authors: Maheshwari A, Christensen RD, Calhoun DA
    Fetal Pediatr Pathol, 2004-03-01;23(2):145-57.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  13. Distinct Compartmentalization of the Chemokines CXCL1 and CXCL2 and the Atypical Receptor ACKR1 Determine Discrete Stages of Neutrophil Diapedesis
    Authors: Tamara Girbl, Tchern Lenn, Lorena Perez, Loïc Rolas, Anna Barkaway, Aude Thiriot et al.
    Immunity
  14. Mesenchymal stem cells promote mammary cancer cell migRation in vitro via the CXCR2 receptor.
    Authors: Halpern Jennifer L, Kilbarger Amy, Lynch Conor C.
    Cancer Letters

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