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Mouse/Rat TNF RI/TNFRSF1A Antibody

Catalog #: MAB425
8 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB425
MAB425-SP
Detection of Mouse and Rat TNF RI/TNFRSF1A by Western Blot.
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Product Details
Citations (8)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat TNF RI/TNFRSF1A Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse TNF RI in ELISAs and Western blots. In ELISAs, no cross-reactivity was observed with recombinant human (rh) TNF RI, rhTNF RII, recombinant mouse TNF RII, rhTNF-alpha, recombinant rat TNF-alpha, recombinant porcine TNF-alpha, or rhTNF-beta.
Source
Monoclonal Rat IgG1 Clone # 47803
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse TNF RI
Ile22-Ala212
Accession # P25118
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Mouse TNF RI/TNFRSF1A Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse TNF RI/TNFRSF1A Biotinylated Antibody (Catalog # BAF425)

Standard: Recombinant Mouse sTNF RI/TNFRSF1A Protein (Catalog # 425-R1)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat TNF RI/TNFRSF1A antibody by Western Blot. View Larger

Detection of Mouse and Rat TNF RI/TNFRSF1A by Western Blot. Western blot shows lysates of C6 rat glioma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse/Rat TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB425) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TNF RI/TNFRSF1A at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF RI/TNFRSF1A

TNF RI functions as a receptor for TNF-alpha and TNF-beta. It is a member of the TNF receptor superfamily and is designated TNFRSF1A.

Long Name
Tumor Necrosis Factor Receptor I
Entrez Gene IDs
7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names
CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor

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Citations for Mouse/Rat TNF RI/TNFRSF1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. The death-inducing activity of RIPK1 is regulated by the pH environment
    Authors: Kenta Moriwaki, Sakthi Balaji, Francis Ka-Ming Ka-Ming Chan
    Science Signaling
  2. Berberine ameliorates lipopolysaccharide?induced inflammatory responses in mouse inner medullary collecting duct?3 cells by downregulation of NF?kappaB pathway
    Authors: DG Kim, JW Choi, IJ Jo, MJ Kim, HS Lee, SH Hong, HJ Song, GS Bae, SJ Park
    Mol Med Rep, 2019-11-19;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  3. FKBP12 mediates necroptosis by initiating RIPK1/RIPK3/MLKL signal transduction in response to TNF receptor 1 ligation
    Authors: Z Wang, J Feng, J Yu, G Chen
    J. Cell. Sci., 2019-05-20;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Pharmacokinetic and Pharmacodynamic Characterisation of an Anti-Mouse TNF Receptor 1 Domain Antibody Formatted for In Vivo Half-Life Extension.
    Authors: Goodall L, Ovecka M, Rycroft D, Friel S, Sanderson A, Mistry P, Davies M, Stoop A
    PLoS ONE, 2015-09-09;10(9):e0137065.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  5. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012-01-25;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  6. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006-08-01;177(3):1967-74.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  7. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005-07-01;302(1):172-81.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  8. Selective macrophage suppression during sepsis.
    Authors: Ellaban E, Bolgos G, Remick D
    Cell. Immunol., 2005-02-26;231(1):103-11.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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